Abstract
Among the foodborne pathogenic bacteria, Salmonella genus was the leading cause of illness-related hospitalizations and deaths. Salmonella is a facultative anaerobic, gram-negative, non-spore-forming, rod-shaped bacterium that belongs to the family of Enterobacteriaceae. Salmonellosis caused by Salmonella usually continues 4-7. days based on health conditions of individuals along with diarrhea, fever, nausea, and abdominal cramps ranging from 12 to 72. h after infection. To prevent and detect Salmonella contaminations in foods, several types of detection methods have been adopted and evaluated. Although traditional culture-based methods have advantages such as low cost and less expensive instrumentation for detection of viable Salmonella strains in foods, they can be time-consuming and labor-intensive. In contrast, molecular-based (DNA or RNA) detection methods exhibit high sensitivity, accuracy than any other method. In this chapter, molecular-based methodologies including several types of polymerase chain reaction assays and whole genome sequencing involving next-generation sequencing platforms will be discussed.
Original language | English |
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Title of host publication | Food and Feed Safety Systems and Analysis |
Publisher | Elsevier |
Pages | 289-308 |
Number of pages | 20 |
ISBN (Electronic) | 9780128498880 |
ISBN (Print) | 9780128118351 |
DOIs | |
State | Published - 2018 |
Bibliographical note
Publisher Copyright:© 2018 Elsevier Inc. All rights reserved.
Keywords
- Detection
- Salmonella
- Whole genome sequencing