TY - CHAP
T1 - Detection Strategies for Foodborne Salmonella and Prospects for Utilization of Whole Genome Sequencing Approaches
AU - Park, Si Hong
AU - Aydin, Mushin
AU - Fan, Peixin
AU - Lee, Shinyoung
AU - Teng, Lin
AU - Kim, Sun Ae
AU - Ahn, Soohyoun
AU - Ricke, Steven C.
AU - Shi, Zhaohao
AU - Jeong, Kwangcheol C.
N1 - Publisher Copyright:
© 2018 Elsevier Inc. All rights reserved.
PY - 2018
Y1 - 2018
N2 - Among the foodborne pathogenic bacteria, Salmonella genus was the leading cause of illness-related hospitalizations and deaths. Salmonella is a facultative anaerobic, gram-negative, non-spore-forming, rod-shaped bacterium that belongs to the family of Enterobacteriaceae. Salmonellosis caused by Salmonella usually continues 4-7. days based on health conditions of individuals along with diarrhea, fever, nausea, and abdominal cramps ranging from 12 to 72. h after infection. To prevent and detect Salmonella contaminations in foods, several types of detection methods have been adopted and evaluated. Although traditional culture-based methods have advantages such as low cost and less expensive instrumentation for detection of viable Salmonella strains in foods, they can be time-consuming and labor-intensive. In contrast, molecular-based (DNA or RNA) detection methods exhibit high sensitivity, accuracy than any other method. In this chapter, molecular-based methodologies including several types of polymerase chain reaction assays and whole genome sequencing involving next-generation sequencing platforms will be discussed.
AB - Among the foodborne pathogenic bacteria, Salmonella genus was the leading cause of illness-related hospitalizations and deaths. Salmonella is a facultative anaerobic, gram-negative, non-spore-forming, rod-shaped bacterium that belongs to the family of Enterobacteriaceae. Salmonellosis caused by Salmonella usually continues 4-7. days based on health conditions of individuals along with diarrhea, fever, nausea, and abdominal cramps ranging from 12 to 72. h after infection. To prevent and detect Salmonella contaminations in foods, several types of detection methods have been adopted and evaluated. Although traditional culture-based methods have advantages such as low cost and less expensive instrumentation for detection of viable Salmonella strains in foods, they can be time-consuming and labor-intensive. In contrast, molecular-based (DNA or RNA) detection methods exhibit high sensitivity, accuracy than any other method. In this chapter, molecular-based methodologies including several types of polymerase chain reaction assays and whole genome sequencing involving next-generation sequencing platforms will be discussed.
KW - Detection
KW - Salmonella
KW - Whole genome sequencing
UR - http://www.scopus.com/inward/record.url?scp=85041249212&partnerID=8YFLogxK
U2 - 10.1016/B978-0-12-811835-1.00016-6
DO - 10.1016/B978-0-12-811835-1.00016-6
M3 - Chapter
AN - SCOPUS:85041249212
SN - 9780128118351
SP - 289
EP - 308
BT - Food and Feed Safety Systems and Analysis
PB - Elsevier
ER -