Detection of thrombin by aptamerbased surface enhanced resonance raman spectroscopy

Hansang Cho; Brian R. Baker; Sebastian Wachsmann-Hogiu; Cynthia Pagba; Ted Laurence; Stephen M. Lane; Luke P. Lee; Jeffrey B.H. Tok

Detection of thrombin by aptamerbased surface enhanced resonance raman spectroscopy

Hansang Cho, Brian R. Baker, Sebastian Wachsmann-Hogiu, Cynthia Pagba, Ted Laurence, Stephen M. Lane, Luke P. Lee, Jeffrey B.H. Tok

Department of Chemistry & Nanoscience

Research output: Contribution to conference › Paper › peer-review

Abstract

This paper reports a selective and sensitive optical detection method using aptamer- based surface enhanced resonance Raman spectroscopy (SERRS). Here we successfully demonstrate thrombin detection down to 100 pM by observing SERRS signal change upon binding. Furthermore, no binding was observed from nonbinding protein or non-thrombin binding aptamer. The aptamer is sufficiently stable to detect 1 nM thrombin in the presence of 10% serum. The proposed detection method may be further implemented for multiplexed detection using different aptamerbased Raman probe complexes.

Original language	English
Pages	290-292
Number of pages	3
State	Published - 2008
Event	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States Duration: 12 Oct 2008 → 16 Oct 2008

Conference

Conference	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
Country/Territory	United States
City	San Diego, CA
Period	12/10/08 → 16/10/08

Keywords

Aptamer
Oligonucleotide
Surface-enhanced resonance raman spectroscopy
Thrombin detection

Cite this

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title = "Detection of thrombin by aptamerbased surface enhanced resonance raman spectroscopy",

abstract = "This paper reports a selective and sensitive optical detection method using aptamer- based surface enhanced resonance Raman spectroscopy (SERRS). Here we successfully demonstrate thrombin detection down to 100 pM by observing SERRS signal change upon binding. Furthermore, no binding was observed from nonbinding protein or non-thrombin binding aptamer. The aptamer is sufficiently stable to detect 1 nM thrombin in the presence of 10% serum. The proposed detection method may be further implemented for multiplexed detection using different aptamerbased Raman probe complexes.",

keywords = "Aptamer, Oligonucleotide, Surface-enhanced resonance raman spectroscopy, Thrombin detection",

author = "Hansang Cho and Baker, {Brian R.} and Sebastian Wachsmann-Hogiu and Cynthia Pagba and Ted Laurence and Lane, {Stephen M.} and Lee, {Luke P.} and Tok, {Jeffrey B.H.}",

year = "2008",

language = "English",

pages = "290--292",

note = "12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 ; Conference date: 12-10-2008 Through 16-10-2008",

}

Detection of thrombin by aptamerbased surface enhanced resonance raman spectroscopy. / Cho, Hansang; Baker, Brian R.; Wachsmann-Hogiu, Sebastian et al.
2008. 290-292 Paper presented at 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States.

Research output: Contribution to conference › Paper › peer-review

TY - CONF

T1 - Detection of thrombin by aptamerbased surface enhanced resonance raman spectroscopy

AU - Cho, Hansang

AU - Baker, Brian R.

AU - Wachsmann-Hogiu, Sebastian

AU - Pagba, Cynthia

AU - Laurence, Ted

AU - Lane, Stephen M.

AU - Lee, Luke P.

AU - Tok, Jeffrey B.H.

PY - 2008

Y1 - 2008

N2 - This paper reports a selective and sensitive optical detection method using aptamer- based surface enhanced resonance Raman spectroscopy (SERRS). Here we successfully demonstrate thrombin detection down to 100 pM by observing SERRS signal change upon binding. Furthermore, no binding was observed from nonbinding protein or non-thrombin binding aptamer. The aptamer is sufficiently stable to detect 1 nM thrombin in the presence of 10% serum. The proposed detection method may be further implemented for multiplexed detection using different aptamerbased Raman probe complexes.

AB - This paper reports a selective and sensitive optical detection method using aptamer- based surface enhanced resonance Raman spectroscopy (SERRS). Here we successfully demonstrate thrombin detection down to 100 pM by observing SERRS signal change upon binding. Furthermore, no binding was observed from nonbinding protein or non-thrombin binding aptamer. The aptamer is sufficiently stable to detect 1 nM thrombin in the presence of 10% serum. The proposed detection method may be further implemented for multiplexed detection using different aptamerbased Raman probe complexes.

KW - Aptamer

KW - Oligonucleotide

KW - Surface-enhanced resonance raman spectroscopy

KW - Thrombin detection

UR - http://www.scopus.com/inward/record.url?scp=84902531170&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:84902531170

SP - 290

EP - 292

T2 - 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008

Y2 - 12 October 2008 through 16 October 2008

ER -

Detection of thrombin by aptamerbased surface enhanced resonance raman spectroscopy

Abstract

Conference

Keywords

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