Detection of kinase activity using versatile fluorescence quencher probes

Hyun Woo Rhee; Seung Hwan Lee; Ik Soo Shin; So Jung Choi; Hun Hee Park; Kyungja Han; Tai Hyun Park; Jong In Hong

doi:10.1002/anie.201000879

Detection of kinase activity using versatile fluorescence quencher probes

Hyun Woo Rhee, Seung Hwan Lee, Ik Soo Shin, So Jung Choi, Hun Hee Park, Kyungja Han, Tai Hyun Park, Jong In Hong

Department of Nutritional Science & Food Management

Research output: Contribution to journal › Article › peer-review

52 Scopus citations

Abstract

(Figure Presented) Turn off the light! A kinase assay system employs fluorescent peptides and a phosphate-selective fluorescence quencher probe, and can be placed on a microfluidic chip (see picture). The system can be used for real-time kinase monitoring, kinase inhibitor screening, and cancer diagnosis based on abnormal kinase activity observed in patients' samples.

Original language	English
Pages (from-to)	4919-4923
Number of pages	5
Journal	Angewandte Chemie - International Edition
Volume	49
Issue number	29
DOIs	https://doi.org/10.1002/anie.201000879
State	Published - 5 Jul 2010

Keywords

Cancer
Fluorescence
Fluorescent probes
Kinases
Phosphorylation

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/anie.201000879

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title = "Detection of kinase activity using versatile fluorescence quencher probes",

abstract = "(Figure Presented) Turn off the light! A kinase assay system employs fluorescent peptides and a phosphate-selective fluorescence quencher probe, and can be placed on a microfluidic chip (see picture). The system can be used for real-time kinase monitoring, kinase inhibitor screening, and cancer diagnosis based on abnormal kinase activity observed in patients' samples.",

keywords = "Cancer, Fluorescence, Fluorescent probes, Kinases, Phosphorylation",

author = "Rhee, \{Hyun Woo\} and Lee, \{Seung Hwan\} and Shin, \{Ik Soo\} and Choi, \{So Jung\} and Park, \{Hun Hee\} and Kyungja Han and Park, \{Tai Hyun\} and Hong, \{Jong In\}",

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T1 - Detection of kinase activity using versatile fluorescence quencher probes

AU - Rhee, Hyun Woo

AU - Lee, Seung Hwan

AU - Shin, Ik Soo

AU - Choi, So Jung

AU - Park, Hun Hee

AU - Han, Kyungja

AU - Park, Tai Hyun

AU - Hong, Jong In

PY - 2010/7/5

Y1 - 2010/7/5

N2 - (Figure Presented) Turn off the light! A kinase assay system employs fluorescent peptides and a phosphate-selective fluorescence quencher probe, and can be placed on a microfluidic chip (see picture). The system can be used for real-time kinase monitoring, kinase inhibitor screening, and cancer diagnosis based on abnormal kinase activity observed in patients' samples.

AB - (Figure Presented) Turn off the light! A kinase assay system employs fluorescent peptides and a phosphate-selective fluorescence quencher probe, and can be placed on a microfluidic chip (see picture). The system can be used for real-time kinase monitoring, kinase inhibitor screening, and cancer diagnosis based on abnormal kinase activity observed in patients' samples.

KW - Cancer

KW - Fluorescence

KW - Fluorescent probes

KW - Kinases

KW - Phosphorylation

UR - https://www.scopus.com/pages/publications/77954318688

U2 - 10.1002/anie.201000879

DO - 10.1002/anie.201000879

M3 - Article

C2 - 20540130

AN - SCOPUS:77954318688

SN - 1433-7851

VL - 49

SP - 4919

EP - 4923

JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

IS - 29

ER -

Detection of kinase activity using versatile fluorescence quencher probes

Abstract

Keywords

UN SDGs

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