Abstract
Here we propose a carbon nanotube (CNT) field-effect transistor (FET) functionalized with aquaporin-4 (AQP4) extracellular loop peptides for the rapid detection of AQP4 antibody without pretreatment. Neuromyelitis optica (NMO) is a rare disease of the central nerve system that affects the optic nerves and the spinal cord. NMO-IgG, a serum antibody in patients, is highly specific for NMO and targets AQP4. We synthesized AQP4 extracellular loop peptides, known as primary autoimmune target in NMO, and immobilized them onto CNT-FET. The sensor showed p-type FET characteristics after the functionalization of peptides. The sensor was able to detect antibody with a detection limit of 1ngl-1. Moreover, AQP4 antibody in human serum was detected without any pretreatment. These results indicate that the biosensor can be used for rapid and simple detection of NMO antibody.
Original language | English |
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Pages (from-to) | 87-91 |
Number of pages | 5 |
Journal | Biosensors and Bioelectronics |
Volume | 78 |
DOIs | |
State | Published - 15 Apr 2016 |
Bibliographical note
Publisher Copyright:© 2015 Elsevier B.V.
Keywords
- Aquaporin-4
- Carbon nanotube
- Field-effect transistor
- Neuromyelitis optica