Detection of aquaporin-4 antibody using aquaporin-4 extracellular loop-based carbon nanotube biosensor for the diagnosis of neuromyelitis optica

Manki Son, Daesan Kim, Kyung Seok Park, Seunghun Hong, Tai Hyun Park

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

Here we propose a carbon nanotube (CNT) field-effect transistor (FET) functionalized with aquaporin-4 (AQP4) extracellular loop peptides for the rapid detection of AQP4 antibody without pretreatment. Neuromyelitis optica (NMO) is a rare disease of the central nerve system that affects the optic nerves and the spinal cord. NMO-IgG, a serum antibody in patients, is highly specific for NMO and targets AQP4. We synthesized AQP4 extracellular loop peptides, known as primary autoimmune target in NMO, and immobilized them onto CNT-FET. The sensor showed p-type FET characteristics after the functionalization of peptides. The sensor was able to detect antibody with a detection limit of 1ngl-1. Moreover, AQP4 antibody in human serum was detected without any pretreatment. These results indicate that the biosensor can be used for rapid and simple detection of NMO antibody.

Original languageEnglish
Pages (from-to)87-91
Number of pages5
JournalBiosensors and Bioelectronics
Volume78
DOIs
StatePublished - 15 Apr 2016

Bibliographical note

Publisher Copyright:
© 2015 Elsevier B.V.

Keywords

  • Aquaporin-4
  • Carbon nanotube
  • Field-effect transistor
  • Neuromyelitis optica

Fingerprint

Dive into the research topics of 'Detection of aquaporin-4 antibody using aquaporin-4 extracellular loop-based carbon nanotube biosensor for the diagnosis of neuromyelitis optica'. Together they form a unique fingerprint.

Cite this