Detection and quantification of toxin-producing microcystis aeruginosa strain in water by nanogene assay

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8 Scopus citations


We demonstrated the quantitative detection of a toxin-producing Microcystis aeruginosa (M. aeruginosa) strain with the laboratory protocol of the NanoGene assay. The NanoGene assay was selected because its laboratory protocol is in the process of being transplanted into a portable system. The mcyD gene of M. aeruginosa was targeted and, as expected, its corresponding fluorescence signal was linearly proportional to the mcyD gene copy number. The sensitivity of the NanoGene assay for this purpose was validated using both dsDNA mcyD gene amplicons and genomic DNAs (gDNA). The limit of detection was determined to be 38 mcyD gene copies per reaction and 9 algal cells/ml water. The specificity of the assay was also demonstrated by the addition of gDNA extracted from environmental algae into the hybridization reaction. Detection of M. aeruginosa was performed in the environmental samples with environmentally relevant sensitivity (~105 algal cells/ml) and specificity. As expected, M. aeruginosa were not detected in nonspecific environmental algal gDNA over the range of 2 × 100 to 2 × 107 algal cells/ml.0 7.

Original languageEnglish
Pages (from-to)808-815
Number of pages8
JournalJournal of Microbiology and Biotechnology
Issue number4
StatePublished - Apr 2017

Bibliographical note

Funding Information:
This project was made possible by funding provided by National Research Foundation of Korea (NRF-2014R1A1A1003129 and NRF-2014R1A1A3051952). The authors appreciate the generous donation of environmental algae samples by Dr. Sang-Don Lee and Hyomin Park at Ewha Womans University.

Publisher Copyright:
© 2017 by The Korean Society for Microbiology and Biotechnology.


  • Algal bloom
  • Cyanobacteria
  • Microcystin
  • Microcystis aeruginosa
  • NanoGene assay


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