Design, synthesis, and evaluation of peptidyl fluorescent probe for Zn2+ in aqueous solution

Bishnu Prasad Joshi, Won Mi Cho, Jongseung Kim, Juyoung Yoon, Keun Hyeung Lee

Research output: Contribution to journalArticlepeer-review

67 Scopus citations

Abstract

A new fluorescent peptide probe for the detection of Zn2+ was synthesized on the basis of zinc binding ligands in zinc enzymes. The peptide that has a unique amino acid sequence displayed a great selectivity for Zn2+ in the presence of several transition metal ions in aqueous solution. The reversibility, binding stoichiometry, binding affinity, and pH sensitivity of the sensor were studied. Further, on-bead application of the peptide as chemosensors was demonstrated.

Original languageEnglish
Pages (from-to)6425-6429
Number of pages5
JournalBioorganic and Medicinal Chemistry Letters
Volume17
Issue number23
DOIs
StatePublished - 1 Dec 2007

Bibliographical note

Funding Information:
This work was supported by the Grant (R01-2006-000-10956-0) from the BasicResearch Program of the Korea Science & Engineering Foundation and the Grant (C00344) from the basic research program of the Korean Research Foundation. B. P. Joshi and Won-Mi Cho were recipients of a BK21 (II) fellowship.

Keywords

  • Dansyl
  • Fluorescence
  • Metal
  • Peptide sensor
  • Probe
  • Selectivity
  • Zn

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