Design, synthesis, and evaluation of peptidyl fluorescent probe for Zn2+ in aqueous solution

Bishnu Prasad Joshi; Won Mi Cho; Jongseung Kim; Juyoung Yoon; Keun Hyeung Lee

doi:10.1016/j.bmcl.2007.10.008

Design, synthesis, and evaluation of peptidyl fluorescent probe for Zn²⁺ in aqueous solution

Bishnu Prasad Joshi, Won Mi Cho, Jongseung Kim, Juyoung Yoon, Keun Hyeung Lee

Department of Chemistry & Nanoscience

Research output: Contribution to journal › Article › peer-review

69 Scopus citations

Abstract

A new fluorescent peptide probe for the detection of Zn²⁺ was synthesized on the basis of zinc binding ligands in zinc enzymes. The peptide that has a unique amino acid sequence displayed a great selectivity for Zn²⁺ in the presence of several transition metal ions in aqueous solution. The reversibility, binding stoichiometry, binding affinity, and pH sensitivity of the sensor were studied. Further, on-bead application of the peptide as chemosensors was demonstrated.

Original language	English
Pages (from-to)	6425-6429
Number of pages	5
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	17
Issue number	23
DOIs	https://doi.org/10.1016/j.bmcl.2007.10.008
State	Published - 1 Dec 2007

Bibliographical note

Funding Information:
This work was supported by the Grant (R01-2006-000-10956-0) from the BasicResearch Program of the Korea Science & Engineering Foundation and the Grant (C00344) from the basic research program of the Korean Research Foundation. B. P. Joshi and Won-Mi Cho were recipients of a BK21 (II) fellowship.

Keywords

Dansyl
Fluorescence
Metal
Peptide sensor
Probe
Selectivity
Zn

Access to Document

10.1016/j.bmcl.2007.10.008

Cite this

@article{75af599341f24265bd381a3e8819acfe,

title = "Design, synthesis, and evaluation of peptidyl fluorescent probe for Zn2+ in aqueous solution",

abstract = "A new fluorescent peptide probe for the detection of Zn2+ was synthesized on the basis of zinc binding ligands in zinc enzymes. The peptide that has a unique amino acid sequence displayed a great selectivity for Zn2+ in the presence of several transition metal ions in aqueous solution. The reversibility, binding stoichiometry, binding affinity, and pH sensitivity of the sensor were studied. Further, on-bead application of the peptide as chemosensors was demonstrated.",

keywords = "Dansyl, Fluorescence, Metal, Peptide sensor, Probe, Selectivity, Zn",

author = "Joshi, {Bishnu Prasad} and Cho, {Won Mi} and Jongseung Kim and Juyoung Yoon and Lee, {Keun Hyeung}",

note = "Funding Information: This work was supported by the Grant (R01-2006-000-10956-0) from the BasicResearch Program of the Korea Science & Engineering Foundation and the Grant (C00344) from the basic research program of the Korean Research Foundation. B. P. Joshi and Won-Mi Cho were recipients of a BK21 (II) fellowship.",

year = "2007",

month = dec,

day = "1",

doi = "10.1016/j.bmcl.2007.10.008",

language = "English",

volume = "17",

pages = "6425--6429",

journal = "Bioorganic and Medicinal Chemistry Letters",

issn = "0960-894X",

publisher = "Elsevier Ltd.",

number = "23",

}

TY - JOUR

T1 - Design, synthesis, and evaluation of peptidyl fluorescent probe for Zn2+ in aqueous solution

AU - Joshi, Bishnu Prasad

AU - Cho, Won Mi

AU - Kim, Jongseung

AU - Yoon, Juyoung

AU - Lee, Keun Hyeung

N1 - Funding Information: This work was supported by the Grant (R01-2006-000-10956-0) from the BasicResearch Program of the Korea Science & Engineering Foundation and the Grant (C00344) from the basic research program of the Korean Research Foundation. B. P. Joshi and Won-Mi Cho were recipients of a BK21 (II) fellowship.

PY - 2007/12/1

Y1 - 2007/12/1

N2 - A new fluorescent peptide probe for the detection of Zn2+ was synthesized on the basis of zinc binding ligands in zinc enzymes. The peptide that has a unique amino acid sequence displayed a great selectivity for Zn2+ in the presence of several transition metal ions in aqueous solution. The reversibility, binding stoichiometry, binding affinity, and pH sensitivity of the sensor were studied. Further, on-bead application of the peptide as chemosensors was demonstrated.

AB - A new fluorescent peptide probe for the detection of Zn2+ was synthesized on the basis of zinc binding ligands in zinc enzymes. The peptide that has a unique amino acid sequence displayed a great selectivity for Zn2+ in the presence of several transition metal ions in aqueous solution. The reversibility, binding stoichiometry, binding affinity, and pH sensitivity of the sensor were studied. Further, on-bead application of the peptide as chemosensors was demonstrated.

KW - Dansyl

KW - Fluorescence

KW - Metal

KW - Peptide sensor

KW - Probe

KW - Selectivity

KW - Zn

UR - http://www.scopus.com/inward/record.url?scp=35648931419&partnerID=8YFLogxK

U2 - 10.1016/j.bmcl.2007.10.008

DO - 10.1016/j.bmcl.2007.10.008

M3 - Article

C2 - 17949975

AN - SCOPUS:35648931419

SN - 0960-894X

VL - 17

SP - 6425

EP - 6429

JO - Bioorganic and Medicinal Chemistry Letters

JF - Bioorganic and Medicinal Chemistry Letters

IS - 23

ER -