TY - JOUR
T1 - Demineralized deciduous tooth as a source of bone graft material
T2 - Its biological and physicochemical characteristics
AU - Park, Mirae
AU - Mah, Yon Joo
AU - Kim, David Hyungjin
AU - Kim, Eun Suk
AU - Park, Eun Jin
N1 - Publisher Copyright:
© 2015 Elsevier Inc.
PY - 2015/9/1
Y1 - 2015/9/1
N2 - Objective To examine structural and physicochemical characteristics of demineralized deciduous tooth powder (DDTP) in relation to demineralization time and to present potential of using DDTP as a bone graft material. Study design For structural and physicochemical analysis, scanning electron microscopy, inductively coupled plasma spectrometry, energy-dispersive X-ray analysis, X-ray diffraction analysis, differential scanning calorimetry, and Brunauer-Emmett-Teller surface area analysis were performed. In in vivo experiments, DDTP was grafted in 20 Sprague-Dawley rats' calvarial defects, and radiographic and histologic examination and histomorphometric analysis were performed. Results In vitro studies confirmed physicochemical demands for collagen-based bone graft material, such as lowered calcium content, lowered crystallinity of hydroxyapatite, and exposed organic structures to demineralization. In vivo experiment indicated new bone formation in DDTP-grafted sites and gradual resorption of the grafted particles. Defect closure rate was significantly higher in the 8-week DDTP-grafted group compared with control (P <.05). Conclusions Deciduous teeth had structural and physicochemical characteristics suitable for grafting with appropriate demineralization. Bone healing was observed to have successfully occurred in DDTP-grafted sites.
AB - Objective To examine structural and physicochemical characteristics of demineralized deciduous tooth powder (DDTP) in relation to demineralization time and to present potential of using DDTP as a bone graft material. Study design For structural and physicochemical analysis, scanning electron microscopy, inductively coupled plasma spectrometry, energy-dispersive X-ray analysis, X-ray diffraction analysis, differential scanning calorimetry, and Brunauer-Emmett-Teller surface area analysis were performed. In in vivo experiments, DDTP was grafted in 20 Sprague-Dawley rats' calvarial defects, and radiographic and histologic examination and histomorphometric analysis were performed. Results In vitro studies confirmed physicochemical demands for collagen-based bone graft material, such as lowered calcium content, lowered crystallinity of hydroxyapatite, and exposed organic structures to demineralization. In vivo experiment indicated new bone formation in DDTP-grafted sites and gradual resorption of the grafted particles. Defect closure rate was significantly higher in the 8-week DDTP-grafted group compared with control (P <.05). Conclusions Deciduous teeth had structural and physicochemical characteristics suitable for grafting with appropriate demineralization. Bone healing was observed to have successfully occurred in DDTP-grafted sites.
UR - http://www.scopus.com/inward/record.url?scp=84939501268&partnerID=8YFLogxK
U2 - 10.1016/j.oooo.2015.05.021
DO - 10.1016/j.oooo.2015.05.021
M3 - Article
C2 - 26297390
AN - SCOPUS:84939501268
SN - 2212-4403
VL - 120
SP - 307
EP - 314
JO - Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
JF - Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
IS - 3
ER -