TY - JOUR
T1 - Degradation characteristics of methyl ethyl ketone by Pseudomonas sp. KT-3 in liquid culture and biofilter
AU - Lee, Tae Ho
AU - Kim, Jaisoo
AU - Kim, Min Joo
AU - Ryu, Hee Wook
AU - Cho, Kyung Suk
N1 - Funding Information:
This work was financially supported by the Korea Science and Engineering Foundation, through the Advanced Environmental Biotechnology Research Center at Pohang University of Science and Technology (R11-2003-006).
PY - 2006/4
Y1 - 2006/4
N2 - With ketone pollution forming an ever-growing problem, it is important to identify a ketone-degrading microorganism and establish its effect. Here, a methyl ethyl ketone (MEK)-degrading bacterium, Pseudomonas sp. KT-3, was isolated and its MEK degradation characteristics were examined in liquid cultures and a polyurethane-packed biofilter. In liquid cultures, strain KT-3 could degrade other ketone solvents, including diethyl ketone (DK), methyl propyl ketone (MPK), methyl isopropyl ketone (MIPK), methyl isobutyl ketone (MIBK), methyl butyl ketone (MBK) and methyl isoamyl ketone (MIAK). The maximum specific growth rate (μmax) of the isolate was 0.136 h -1 in MEK medium supplemented with MEK as a sole carbon source, and kinetically, the maximum removal rate (Vm) and saturation constant (Km) for MEK were 12.28 mM g-1 DCW h-1 (DCW: dry cell weight) and 1.64 mM, respectively. MEK biodegradation by KT-3 was suppressed by the addition of MIBK or acetone, but not by toluene. In the tested biofilter, KT-3 exhibited a > 90% removal efficiency for MEK inlet concentrations of around 500 ppmv at a space velocity (SV) of 150 h -1. The elimination capacity of MEK was more influenced by SV than by the inlet concentration. Kinetic analysis showed that the maximum MEK removal rate (Vm) was 690 g m-3 h-1 and the saturation constant (Km) was 490 ppmv. Collectively, these results indicate the polyurethane sequencing batch biofilter with Pseudomonas sp. KT-3 will provide an excellent performance in the removal of gaseous MEK.
AB - With ketone pollution forming an ever-growing problem, it is important to identify a ketone-degrading microorganism and establish its effect. Here, a methyl ethyl ketone (MEK)-degrading bacterium, Pseudomonas sp. KT-3, was isolated and its MEK degradation characteristics were examined in liquid cultures and a polyurethane-packed biofilter. In liquid cultures, strain KT-3 could degrade other ketone solvents, including diethyl ketone (DK), methyl propyl ketone (MPK), methyl isopropyl ketone (MIPK), methyl isobutyl ketone (MIBK), methyl butyl ketone (MBK) and methyl isoamyl ketone (MIAK). The maximum specific growth rate (μmax) of the isolate was 0.136 h -1 in MEK medium supplemented with MEK as a sole carbon source, and kinetically, the maximum removal rate (Vm) and saturation constant (Km) for MEK were 12.28 mM g-1 DCW h-1 (DCW: dry cell weight) and 1.64 mM, respectively. MEK biodegradation by KT-3 was suppressed by the addition of MIBK or acetone, but not by toluene. In the tested biofilter, KT-3 exhibited a > 90% removal efficiency for MEK inlet concentrations of around 500 ppmv at a space velocity (SV) of 150 h -1. The elimination capacity of MEK was more influenced by SV than by the inlet concentration. Kinetic analysis showed that the maximum MEK removal rate (Vm) was 690 g m-3 h-1 and the saturation constant (Km) was 490 ppmv. Collectively, these results indicate the polyurethane sequencing batch biofilter with Pseudomonas sp. KT-3 will provide an excellent performance in the removal of gaseous MEK.
KW - Biodegradation
KW - Biofilter
KW - Ketones
KW - Methyl ethyl ketone (MEK)
KW - Pseudomonas sp. KT-3
UR - http://www.scopus.com/inward/record.url?scp=33645305429&partnerID=8YFLogxK
U2 - 10.1016/j.chemosphere.2005.07.067
DO - 10.1016/j.chemosphere.2005.07.067
M3 - Article
C2 - 16169047
AN - SCOPUS:33645305429
SN - 0045-6535
VL - 63
SP - 315
EP - 322
JO - Chemosphere
JF - Chemosphere
IS - 2
ER -