TY - JOUR
T1 - Decreased syndecan-2 expression correlates with trichostatin-A induced-morphological changes and reduced tumorigenic activity in colon carcinoma cells
AU - Kim, Yeonhee
AU - Park, Haein
AU - Lim, Yangmi
AU - Han, Innoc
AU - Ho, Jeong Kwon
AU - Woods, Anne
AU - Oh, Eok Soo
N1 - Funding Information:
This work was supported by a research grant of the Ministry of Health and Welfare (HMP-00-B-20800-0080) to ESO, and the Korea Health 21 R&D Project,
PY - 2003/2/13
Y1 - 2003/2/13
N2 - The inhibition of histone deacetylase activity is known to induce morphological changes of transformed cells. In this study, we investigated the effect of the specific HDAC inhibitor, trichostatin A (TSA), on colon carcinoma cell lines. Treatment of human colorectal carcinoma cells, KM1214 and KM12SM, with TSA induced distinct morphological changes. Both cell lines, which normally piled up in layers without clear boundary, became more flattened, and formed monolayers with evident boundaries between cells, with concomitant increased actin filament organization. Cell-cell interaction was not affected much, based on expression level, membrane localization, and interaction of E-cadherin with β-catenin. In contrast, syndecan-2 expression was dramatically reduced and it was correlated with the morphological changes of colon carcinoma cells. Consistently, downregulation of syndecan-2 expression by antisense cDNA clearly mimicked the morphological changes in KM12SM and reduced anchorage-independent growth of colon cancer cells. All these results indicate that reduced syndecan-2 expression correlates with TSA-induced morphological changes and reduced tumorigenic activity in colon carcinoma cells.
AB - The inhibition of histone deacetylase activity is known to induce morphological changes of transformed cells. In this study, we investigated the effect of the specific HDAC inhibitor, trichostatin A (TSA), on colon carcinoma cell lines. Treatment of human colorectal carcinoma cells, KM1214 and KM12SM, with TSA induced distinct morphological changes. Both cell lines, which normally piled up in layers without clear boundary, became more flattened, and formed monolayers with evident boundaries between cells, with concomitant increased actin filament organization. Cell-cell interaction was not affected much, based on expression level, membrane localization, and interaction of E-cadherin with β-catenin. In contrast, syndecan-2 expression was dramatically reduced and it was correlated with the morphological changes of colon carcinoma cells. Consistently, downregulation of syndecan-2 expression by antisense cDNA clearly mimicked the morphological changes in KM12SM and reduced anchorage-independent growth of colon cancer cells. All these results indicate that reduced syndecan-2 expression correlates with TSA-induced morphological changes and reduced tumorigenic activity in colon carcinoma cells.
KW - Colon carcinoma
KW - Histone deacetylase syndecan-2
KW - Tumorigenesis
UR - http://www.scopus.com/inward/record.url?scp=0037434784&partnerID=8YFLogxK
U2 - 10.1038/sj.onc.1206068
DO - 10.1038/sj.onc.1206068
M3 - Article
C2 - 12584561
AN - SCOPUS:0037434784
SN - 0950-9232
VL - 22
SP - 826
EP - 830
JO - Oncogene
JF - Oncogene
IS - 6
ER -