Dark-field smartphone microscope with nanoscale resolution for molecular diagnostics

Brian N. Kim; Julian A. Diaz; Soon Gweon Hong; Sang Hun Lee; Luke P. Lee

Dark-field smartphone microscope with nanoscale resolution for molecular diagnostics

Brian N. Kim
, Julian A. Diaz
, Soon Gweon Hong
, Sang Hun Lee
, Luke P. Lee

Department of Chemistry & Nanoscience

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

8 Scopus citations

Abstract

Dark-field microscopy allows highly sensitive detection of metal nanoparticles that exhibit surface plasmon resonance (SPR) scattering by exclusively collecting scattered light. Here, we report a dark-field smartphone microscope with single nanoparticle resolution for point-of-care testing (POCT). A simple flat-lens LED is coupled to transparent substrate to generate total internal reflection (TIR) and evanescent field for surface-confined illumination, which scatters at nanoparticles. The scattering light is detected using smartphone camera through an external lens attachment. Dark-field smartphone microscope will facilitate the rapid translation of sensitive nanoparticle-based bioassays to point-of-care testing (POCT).

Original language	English
Title of host publication	18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
Publisher	Chemical and Biological Microsystems Society
Pages	2247-2249
Number of pages	3
ISBN (Electronic)	9780979806476
State	Published - 2014
Event	18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States Duration: 26 Oct 2014 → 30 Oct 2014

Publication series

Name	18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

Conference

Conference	18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
Country/Territory	United States
City	San Antonio
Period	26/10/14 → 30/10/14

Bibliographical note

Publisher Copyright:
© 14CBMS.

Keywords

Dark-field microscopy
Nanoparticle
Point-of-care testing (POCT)
Smartphone microscopy
Surface plasmon resonance (SPR)
Total internal reflection (TIR)

Cite this

Kim, B. N., Diaz, J. A., Hong, S. G., Lee, S. H., & Lee, L. P. (2014). Dark-field smartphone microscope with nanoscale resolution for molecular diagnostics. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 (pp. 2247-2249). (18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014). Chemical and Biological Microsystems Society.

Kim, Brian N. ; Diaz, Julian A. ; Hong, Soon Gweon et al. / Dark-field smartphone microscope with nanoscale resolution for molecular diagnostics. 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, 2014. pp. 2247-2249 (18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014).

@inproceedings{9d39b26e330c4f13bcceec7911f5276c,

title = "Dark-field smartphone microscope with nanoscale resolution for molecular diagnostics",

abstract = "Dark-field microscopy allows highly sensitive detection of metal nanoparticles that exhibit surface plasmon resonance (SPR) scattering by exclusively collecting scattered light. Here, we report a dark-field smartphone microscope with single nanoparticle resolution for point-of-care testing (POCT). A simple flat-lens LED is coupled to transparent substrate to generate total internal reflection (TIR) and evanescent field for surface-confined illumination, which scatters at nanoparticles. The scattering light is detected using smartphone camera through an external lens attachment. Dark-field smartphone microscope will facilitate the rapid translation of sensitive nanoparticle-based bioassays to point-of-care testing (POCT).",

keywords = "Dark-field microscopy, Nanoparticle, Point-of-care testing (POCT), Smartphone microscopy, Surface plasmon resonance (SPR), Total internal reflection (TIR)",

author = "Kim, \{Brian N.\} and Diaz, \{Julian A.\} and Hong, \{Soon Gweon\} and Lee, \{Sang Hun\} and Lee, \{Luke P.\}",

note = "Publisher Copyright: {\textcopyright} 14CBMS.; 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 ; Conference date: 26-10-2014 Through 30-10-2014",

year = "2014",

language = "English",

series = "18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014",

publisher = "Chemical and Biological Microsystems Society",

pages = "2247--2249",

booktitle = "18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014",

}

Kim, BN, Diaz, JA, Hong, SG, Lee, SH & Lee, LP 2014, Dark-field smartphone microscope with nanoscale resolution for molecular diagnostics. in 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, Chemical and Biological Microsystems Society, pp. 2247-2249, 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, San Antonio, United States, 26/10/14.

Dark-field smartphone microscope with nanoscale resolution for molecular diagnostics. / Kim, Brian N.; Diaz, Julian A.; Hong, Soon Gweon et al.
18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, 2014. p. 2247-2249 (18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Dark-field smartphone microscope with nanoscale resolution for molecular diagnostics

AU - Kim, Brian N.

AU - Diaz, Julian A.

AU - Hong, Soon Gweon

AU - Lee, Sang Hun

AU - Lee, Luke P.

N1 - Publisher Copyright: © 14CBMS.

PY - 2014

Y1 - 2014

N2 - Dark-field microscopy allows highly sensitive detection of metal nanoparticles that exhibit surface plasmon resonance (SPR) scattering by exclusively collecting scattered light. Here, we report a dark-field smartphone microscope with single nanoparticle resolution for point-of-care testing (POCT). A simple flat-lens LED is coupled to transparent substrate to generate total internal reflection (TIR) and evanescent field for surface-confined illumination, which scatters at nanoparticles. The scattering light is detected using smartphone camera through an external lens attachment. Dark-field smartphone microscope will facilitate the rapid translation of sensitive nanoparticle-based bioassays to point-of-care testing (POCT).

AB - Dark-field microscopy allows highly sensitive detection of metal nanoparticles that exhibit surface plasmon resonance (SPR) scattering by exclusively collecting scattered light. Here, we report a dark-field smartphone microscope with single nanoparticle resolution for point-of-care testing (POCT). A simple flat-lens LED is coupled to transparent substrate to generate total internal reflection (TIR) and evanescent field for surface-confined illumination, which scatters at nanoparticles. The scattering light is detected using smartphone camera through an external lens attachment. Dark-field smartphone microscope will facilitate the rapid translation of sensitive nanoparticle-based bioassays to point-of-care testing (POCT).

KW - Dark-field microscopy

KW - Nanoparticle

KW - Point-of-care testing (POCT)

KW - Smartphone microscopy

KW - Surface plasmon resonance (SPR)

KW - Total internal reflection (TIR)

UR - https://www.scopus.com/pages/publications/84941649289

M3 - Conference contribution

AN - SCOPUS:84941649289

T3 - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

SP - 2247

EP - 2249

BT - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

PB - Chemical and Biological Microsystems Society

T2 - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

Y2 - 26 October 2014 through 30 October 2014

ER -

Kim BN, Diaz JA, Hong SG, Lee SH, Lee LP. Dark-field smartphone microscope with nanoscale resolution for molecular diagnostics. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society. 2014. p. 2247-2249. (18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014).

Dark-field smartphone microscope with nanoscale resolution for molecular diagnostics

Abstract

Publication series

Conference

Bibliographical note

Keywords

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Cite this