Abstract
Dark-field microscopy allows highly sensitive detection of metal nanoparticles that exhibit surface plasmon resonance (SPR) scattering by exclusively collecting scattered light. Here, we report a dark-field smartphone microscope with single nanoparticle resolution for point-of-care testing (POCT). A simple flat-lens LED is coupled to transparent substrate to generate total internal reflection (TIR) and evanescent field for surface-confined illumination, which scatters at nanoparticles. The scattering light is detected using smartphone camera through an external lens attachment. Dark-field smartphone microscope will facilitate the rapid translation of sensitive nanoparticle-based bioassays to point-of-care testing (POCT).
| Original language | English |
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| Title of host publication | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 2247-2249 |
| Number of pages | 3 |
| ISBN (Electronic) | 9780979806476 |
| State | Published - 2014 |
| Event | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States Duration: 26 Oct 2014 → 30 Oct 2014 |
Publication series
| Name | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 |
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Conference
| Conference | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 |
|---|---|
| Country/Territory | United States |
| City | San Antonio |
| Period | 26/10/14 → 30/10/14 |
Bibliographical note
Publisher Copyright:© 14CBMS.
Keywords
- Dark-field microscopy
- Nanoparticle
- Point-of-care testing (POCT)
- Smartphone microscopy
- Surface plasmon resonance (SPR)
- Total internal reflection (TIR)