Daily nutritional dose supplementation with antioxidant nutrients and phytochemicals improves DNA and LDL stability: A double-blind, randomized, and placebo-controlled trial

You Jin Kim, Yoon Hee Ahn, Yeni Lim, Ji Yeon Kim, Joohee Kim, Oran Kwon

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Reactive oxygen species are important risk factors for age-related diseases, but they also act as signaling factors for endogenous antioxidative defense. The hypothesis that a multi-micronutrient supplement with nutritional doses of antioxidant nutrients and phytochemicals (MP) may provide protection against oxidative damage and maintain the endogenous antioxidant defense capacity was assessed in subjects with a habitually low intake of fruits and vegetables. In a randomized, placebo-controlled, and parallel designed trial, 89 eligible subjects were assigned to either placebo or MP for eight weeks. Eighty subjects have completed the protocol and included for the analysis. MP treatment was superior at increasing serum folate (p < 0.0001) and resistance to DNA damage (p = 0.006, tail intensity; p = 0.030, tail moment by comet assay), and LDL oxidation (p = 0.009) compared with the placebo. Moreover, the endogenous oxidative defense capacity was not weakened after MP supplementation, as determined by the levels of glutathione peroxidase (p = 0.442), catalase (p = 0.686), and superoxide dismutase (p = 0.804). The serum folate level was negatively correlated with DNA damage (r = -0.376, p = 0.001 for tail density; r = -0.329, p = 0.003 for tail moment), but no correlation was found with LDL oxidation (r = -0.123, p = 0.275). These results suggest that MP use in healthy subjects with habitually low dietary fruit and vegetable intake may be beneficial in providing resistance to oxidative damage to DNA and LDL without suppressing the endogenous defense mechanisms.

Original languageEnglish
Pages (from-to)5218-5232
Number of pages15
JournalNutrients
Volume5
Issue number12
DOIs
StatePublished - 18 Dec 2013

Keywords

  • Human intervention
  • Multi-micronutrient supplement
  • Nutritional dose
  • Phytochemicals

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