Cytotoxic induction and photoacoustic imaging of breast cancer cells using astaxanthin-reduced gold nanoparticles

Subramaniyan Bharathiraja, Panchanathan Manivasagan, Nhat Quang Bui, Yun Ok Oh, In Gweon Lim, Suhyun Park, Junghwan Oh

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

Astaxanthin, a kind of photosynthetic pigment, was employed for gold nanoparticle formation. Nanoparticles were characterized using Ulteraviolet-Visible (UV-Vis) spectroscopy, transmission electron microscopy, and X-ray diffraction, and the possible presence of astaxanthin functional groups were analyzed by Fourier transform infrared spectroscopy (FTIR). The cytotoxic effect of synthesized nanoparticles was evaluated against MDA-MB-231 (human breast cancer cells) using a tetrazolium-based assay, and synthesized nanoparticles exhibited dose-dependent toxicity. The morphology upon cell death was differentiated through fluorescent microscopy using different stains that predicted apoptosis. The synthesized nanoparticles were applied in ultrasound-coupled photoacoustic imaging to obtain good images of treated cells. Astaxanthin-reduced gold nanoparticle has the potential to act as a promising agent in the field of photo-based diagnosis and therapy.

Original languageEnglish
Article number78
JournalNanomaterials
Volume6
Issue number4
DOIs
StatePublished - 20 Apr 2016

Keywords

  • Astaxanthin
  • Breast cancer
  • Cytotoxicity
  • Gold nanoparticle
  • Photoacoustic imaging

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