Cytotoxic effect of 7 β-Hydroxycholesterol on human NCI-H460 lung cancer cells

Kyoung Ah Kang, Sungwook Chae, Kyoung Hwa Lee, Moon Taek Park, Su Jae Lee, Yun Sil Lee, Jin Won Hyun

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15 Scopus citations

Abstract

The cytotoxic activity of 7β-hydroxycholesterol (7β-OHC) was evaluated on human NCI-H460 lung cancer cells. 7β-OHC decreased clonogenic survival of NCI-H460 in a dose dependent pattern. 7β-OHC induced apoptosis in NCI-H460, with the characteristic features like increase in sub-G1 hypodiploid (apoptotic) cells, and apoptotic body formation, as evidenced by flow cytometry and fluorescence microscope, respectively. Apoptosis was also associated with loss of mitochondrial transmembrane potential, and the activation of caspases 9 and 3. 7β-OHC resulted in generation of reactive oxygen species (ROS) during apoptosis. On the whole, the results indicated that 7β-OHC inhibited the proliferation of NCI-H460 cells through apoptosis via caspase activation.

Original languageEnglish
Pages (from-to)1377-1380
Number of pages4
JournalBiological and Pharmaceutical Bulletin
Volume28
Issue number8
DOIs
StatePublished - Aug 2005

Keywords

  • 7β-hydroxycholesterol
  • Clonogenic survival
  • NCI-H460 lung cancer cell

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