Cytotoxic effect of 7 β-Hydroxycholesterol on human NCI-H460 lung cancer cells

Kyoung Ah Kang; Sungwook Chae; Kyoung Hwa Lee; Moon Taek Park; Su Jae Lee; Yun Sil Lee; Jin Won Hyun

doi:10.1248/bpb.28.1377

Cytotoxic effect of 7 β-Hydroxycholesterol on human NCI-H460 lung cancer cells

Kyoung Ah Kang, Sungwook Chae, Kyoung Hwa Lee, Moon Taek Park, Su Jae Lee, Yun Sil Lee, Jin Won Hyun

Department of Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

The cytotoxic activity of 7β-hydroxycholesterol (7β-OHC) was evaluated on human NCI-H460 lung cancer cells. 7β-OHC decreased clonogenic survival of NCI-H460 in a dose dependent pattern. 7β-OHC induced apoptosis in NCI-H460, with the characteristic features like increase in sub-G₁ hypodiploid (apoptotic) cells, and apoptotic body formation, as evidenced by flow cytometry and fluorescence microscope, respectively. Apoptosis was also associated with loss of mitochondrial transmembrane potential, and the activation of caspases 9 and 3. 7β-OHC resulted in generation of reactive oxygen species (ROS) during apoptosis. On the whole, the results indicated that 7β-OHC inhibited the proliferation of NCI-H460 cells through apoptosis via caspase activation.

Original language	English
Pages (from-to)	1377-1380
Number of pages	4
Journal	Biological and Pharmaceutical Bulletin
Volume	28
Issue number	8
DOIs	https://doi.org/10.1248/bpb.28.1377
State	Published - Aug 2005

Keywords

7β-hydroxycholesterol
Clonogenic survival
NCI-H460 lung cancer cell

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1248/bpb.28.1377

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title = "Cytotoxic effect of 7 β-Hydroxycholesterol on human NCI-H460 lung cancer cells",

abstract = "The cytotoxic activity of 7β-hydroxycholesterol (7β-OHC) was evaluated on human NCI-H460 lung cancer cells. 7β-OHC decreased clonogenic survival of NCI-H460 in a dose dependent pattern. 7β-OHC induced apoptosis in NCI-H460, with the characteristic features like increase in sub-G1 hypodiploid (apoptotic) cells, and apoptotic body formation, as evidenced by flow cytometry and fluorescence microscope, respectively. Apoptosis was also associated with loss of mitochondrial transmembrane potential, and the activation of caspases 9 and 3. 7β-OHC resulted in generation of reactive oxygen species (ROS) during apoptosis. On the whole, the results indicated that 7β-OHC inhibited the proliferation of NCI-H460 cells through apoptosis via caspase activation.",

keywords = "7β-hydroxycholesterol, Clonogenic survival, NCI-H460 lung cancer cell",

author = "Kang, {Kyoung Ah} and Sungwook Chae and Lee, {Kyoung Hwa} and Park, {Moon Taek} and Lee, {Su Jae} and Lee, {Yun Sil} and Hyun, {Jin Won}",

year = "2005",

month = aug,

doi = "10.1248/bpb.28.1377",

language = "English",

volume = "28",

pages = "1377--1380",

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T1 - Cytotoxic effect of 7 β-Hydroxycholesterol on human NCI-H460 lung cancer cells

AU - Kang, Kyoung Ah

AU - Chae, Sungwook

AU - Lee, Kyoung Hwa

AU - Park, Moon Taek

AU - Lee, Su Jae

AU - Lee, Yun Sil

AU - Hyun, Jin Won

PY - 2005/8

Y1 - 2005/8

N2 - The cytotoxic activity of 7β-hydroxycholesterol (7β-OHC) was evaluated on human NCI-H460 lung cancer cells. 7β-OHC decreased clonogenic survival of NCI-H460 in a dose dependent pattern. 7β-OHC induced apoptosis in NCI-H460, with the characteristic features like increase in sub-G1 hypodiploid (apoptotic) cells, and apoptotic body formation, as evidenced by flow cytometry and fluorescence microscope, respectively. Apoptosis was also associated with loss of mitochondrial transmembrane potential, and the activation of caspases 9 and 3. 7β-OHC resulted in generation of reactive oxygen species (ROS) during apoptosis. On the whole, the results indicated that 7β-OHC inhibited the proliferation of NCI-H460 cells through apoptosis via caspase activation.

AB - The cytotoxic activity of 7β-hydroxycholesterol (7β-OHC) was evaluated on human NCI-H460 lung cancer cells. 7β-OHC decreased clonogenic survival of NCI-H460 in a dose dependent pattern. 7β-OHC induced apoptosis in NCI-H460, with the characteristic features like increase in sub-G1 hypodiploid (apoptotic) cells, and apoptotic body formation, as evidenced by flow cytometry and fluorescence microscope, respectively. Apoptosis was also associated with loss of mitochondrial transmembrane potential, and the activation of caspases 9 and 3. 7β-OHC resulted in generation of reactive oxygen species (ROS) during apoptosis. On the whole, the results indicated that 7β-OHC inhibited the proliferation of NCI-H460 cells through apoptosis via caspase activation.

KW - 7β-hydroxycholesterol

KW - Clonogenic survival

KW - NCI-H460 lung cancer cell

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Cytotoxic effect of 7 β-Hydroxycholesterol on human NCI-H460 lung cancer cells

Abstract

Keywords

UN SDGs

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