Cytoprotective effects of KIOM-79 on streptozotocin induced cell damage by inhibiting ERK and AP-1

Kyoung Ah Kang; Kyoung Hwa Lee; So Young Kim; Hee Sun Kim; Jin Sook Kim; Jin Won Hyun

doi:10.1248/bpb.30.852

Cytoprotective effects of KIOM-79 on streptozotocin induced cell damage by inhibiting ERK and AP-1

Kyoung Ah Kang, Kyoung Hwa Lee, So Young Kim, Hee Sun Kim, Jin Sook Kim, Jin Won Hyun

Department of Molecular Medicine

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

The present study investigated the potential cytoprotective properties of a combination of plant extracts (KIOM-79) obtained from Magnolia officinalis, Pueraria lobata, Glycyrrhiza uralensis, and Euphorbia pekinensis, against the oxidative stresses induced by streptozotocin (STZ) in a rat pancreatic β-cells (RINm5F). KIOM-79 was found to scavenge intracellular reactive oxygen species (ROS), thereby preventing DNA damage and lipid peroxidation. The KIOM-79 inhibited apoptosis of the β-cells exposed to STZ via radical scavenging activity and activation of antioxidant enzymes. KIOM-79 inhibited activation of extracellular regulated kinase (ERK) induced by STZ and inhibited DNA binding activity of an activator protein-1 (AP-1), a downstream transcription factor of ERK. Taken together, these findings suggest that KIOM-79 protects against STZ induced cell death in RINm5F cells by inhibiting ROS generation and the ERK pathway.

Original language	English
Pages (from-to)	852-858
Number of pages	7
Journal	Biological and Pharmaceutical Bulletin
Volume	30
Issue number	5
DOIs	https://doi.org/10.1248/bpb.30.852
State	Published - May 2007

Keywords

Cytoprotective property
KIOM-79
Oxidative stress
Reactive oxygen species

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title = "Cytoprotective effects of KIOM-79 on streptozotocin induced cell damage by inhibiting ERK and AP-1",

abstract = "The present study investigated the potential cytoprotective properties of a combination of plant extracts (KIOM-79) obtained from Magnolia officinalis, Pueraria lobata, Glycyrrhiza uralensis, and Euphorbia pekinensis, against the oxidative stresses induced by streptozotocin (STZ) in a rat pancreatic β-cells (RINm5F). KIOM-79 was found to scavenge intracellular reactive oxygen species (ROS), thereby preventing DNA damage and lipid peroxidation. The KIOM-79 inhibited apoptosis of the β-cells exposed to STZ via radical scavenging activity and activation of antioxidant enzymes. KIOM-79 inhibited activation of extracellular regulated kinase (ERK) induced by STZ and inhibited DNA binding activity of an activator protein-1 (AP-1), a downstream transcription factor of ERK. Taken together, these findings suggest that KIOM-79 protects against STZ induced cell death in RINm5F cells by inhibiting ROS generation and the ERK pathway.",

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author = "Kang, {Kyoung Ah} and Lee, {Kyoung Hwa} and Kim, {So Young} and Kim, {Hee Sun} and Kim, {Jin Sook} and Hyun, {Jin Won}",

year = "2007",

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AU - Kang, Kyoung Ah

AU - Lee, Kyoung Hwa

AU - Kim, So Young

AU - Kim, Hee Sun

AU - Kim, Jin Sook

AU - Hyun, Jin Won

PY - 2007/5

Y1 - 2007/5

N2 - The present study investigated the potential cytoprotective properties of a combination of plant extracts (KIOM-79) obtained from Magnolia officinalis, Pueraria lobata, Glycyrrhiza uralensis, and Euphorbia pekinensis, against the oxidative stresses induced by streptozotocin (STZ) in a rat pancreatic β-cells (RINm5F). KIOM-79 was found to scavenge intracellular reactive oxygen species (ROS), thereby preventing DNA damage and lipid peroxidation. The KIOM-79 inhibited apoptosis of the β-cells exposed to STZ via radical scavenging activity and activation of antioxidant enzymes. KIOM-79 inhibited activation of extracellular regulated kinase (ERK) induced by STZ and inhibited DNA binding activity of an activator protein-1 (AP-1), a downstream transcription factor of ERK. Taken together, these findings suggest that KIOM-79 protects against STZ induced cell death in RINm5F cells by inhibiting ROS generation and the ERK pathway.

AB - The present study investigated the potential cytoprotective properties of a combination of plant extracts (KIOM-79) obtained from Magnolia officinalis, Pueraria lobata, Glycyrrhiza uralensis, and Euphorbia pekinensis, against the oxidative stresses induced by streptozotocin (STZ) in a rat pancreatic β-cells (RINm5F). KIOM-79 was found to scavenge intracellular reactive oxygen species (ROS), thereby preventing DNA damage and lipid peroxidation. The KIOM-79 inhibited apoptosis of the β-cells exposed to STZ via radical scavenging activity and activation of antioxidant enzymes. KIOM-79 inhibited activation of extracellular regulated kinase (ERK) induced by STZ and inhibited DNA binding activity of an activator protein-1 (AP-1), a downstream transcription factor of ERK. Taken together, these findings suggest that KIOM-79 protects against STZ induced cell death in RINm5F cells by inhibiting ROS generation and the ERK pathway.

KW - Cytoprotective property

KW - KIOM-79

KW - Oxidative stress

KW - Reactive oxygen species

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JO - Biological and Pharmaceutical Bulletin

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IS - 5

ER -

Cytoprotective effects of KIOM-79 on streptozotocin induced cell damage by inhibiting ERK and AP-1

Abstract

Keywords

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