TY - JOUR
T1 - Cytoprotective effect of tectorigenin, a metabolite formed by transformation of tectoridin by intestinal microflora, on oxidative stress induced by hydrogen peroxide
AU - Kang, Kyoung Ah
AU - Lee, Kyoung Hwa
AU - Chae, Sungwook
AU - Zhang, Rui
AU - Jung, Myung Sun
AU - Kim, So Young
AU - Kim, Hee Sun
AU - Kim, Dong Hyun
AU - Hyun, Jin Won
N1 - Funding Information:
This study was supported by a grant from Korea Science and Engineering Foundation (KOSEF), and Ministry of Science and Technology (MOST), Korean government.
PY - 2005/9/5
Y1 - 2005/9/5
N2 - In the present study, the antioxidative properties of tectorigenin, a metabolite formed by transformation of tectoridin by intestinal microflora, were investigated. Tectorigenin was found to scavenge intracellular reactive oxygen species, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, and thus prevented lipid peroxidation. The radical scavenging activity of tectorigenin protected the viability of Chinese hamster lung fibroblast (V79-4) cells exposed to hydrogen peroxide (H2O2) via activation of extracellular signal regulated kinase (ERK) pathway. Furthermore, tectorigenin reduced the apoptotic cells formation and cell cycle arrest at G2/M phase induced by H2O2. Tectorigenin increased the activities of cellular antioxidant enzymes like superoxide dismutase, catalase, glutathione peroxidase, and also increased their protein level. Taken together, these findings suggest that tectorigenin protected V79-4 cells against H 2O2 damage, by enhancing the antioxidative activity and by activating ERK pathway.
AB - In the present study, the antioxidative properties of tectorigenin, a metabolite formed by transformation of tectoridin by intestinal microflora, were investigated. Tectorigenin was found to scavenge intracellular reactive oxygen species, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, and thus prevented lipid peroxidation. The radical scavenging activity of tectorigenin protected the viability of Chinese hamster lung fibroblast (V79-4) cells exposed to hydrogen peroxide (H2O2) via activation of extracellular signal regulated kinase (ERK) pathway. Furthermore, tectorigenin reduced the apoptotic cells formation and cell cycle arrest at G2/M phase induced by H2O2. Tectorigenin increased the activities of cellular antioxidant enzymes like superoxide dismutase, catalase, glutathione peroxidase, and also increased their protein level. Taken together, these findings suggest that tectorigenin protected V79-4 cells against H 2O2 damage, by enhancing the antioxidative activity and by activating ERK pathway.
KW - Antioxidant enzyme
KW - Reactive oxygen species
KW - Tectorigenin
UR - http://www.scopus.com/inward/record.url?scp=27744497606&partnerID=8YFLogxK
U2 - 10.1016/j.ejphar.2005.06.043
DO - 10.1016/j.ejphar.2005.06.043
M3 - Article
C2 - 16102749
AN - SCOPUS:27744497606
SN - 0014-2999
VL - 519
SP - 16
EP - 23
JO - European Journal of Pharmacology
JF - European Journal of Pharmacology
IS - 1-2
ER -