Cytoprotective effect of tectorigenin, a metabolite formed by transformation of tectoridin by intestinal microflora, on oxidative stress induced by hydrogen peroxide

Kyoung Ah Kang; Kyoung Hwa Lee; Sungwook Chae; Rui Zhang; Myung Sun Jung; So Young Kim; Hee Sun Kim; Dong Hyun Kim; Jin Won Hyun

doi:10.1016/j.ejphar.2005.06.043

Cytoprotective effect of tectorigenin, a metabolite formed by transformation of tectoridin by intestinal microflora, on oxidative stress induced by hydrogen peroxide

Kyoung Ah Kang, Kyoung Hwa Lee, Sungwook Chae, Rui Zhang, Myung Sun Jung, So Young Kim, Hee Sun Kim, Dong Hyun Kim, Jin Won Hyun

Department of Molecular Medicine

Research output: Contribution to journal › Article › peer-review

55 Scopus citations

Abstract

In the present study, the antioxidative properties of tectorigenin, a metabolite formed by transformation of tectoridin by intestinal microflora, were investigated. Tectorigenin was found to scavenge intracellular reactive oxygen species, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, and thus prevented lipid peroxidation. The radical scavenging activity of tectorigenin protected the viability of Chinese hamster lung fibroblast (V79-4) cells exposed to hydrogen peroxide (H₂O₂) via activation of extracellular signal regulated kinase (ERK) pathway. Furthermore, tectorigenin reduced the apoptotic cells formation and cell cycle arrest at G₂/M phase induced by H₂O₂. Tectorigenin increased the activities of cellular antioxidant enzymes like superoxide dismutase, catalase, glutathione peroxidase, and also increased their protein level. Taken together, these findings suggest that tectorigenin protected V79-4 cells against H ₂O₂ damage, by enhancing the antioxidative activity and by activating ERK pathway.

Original language	English
Pages (from-to)	16-23
Number of pages	8
Journal	European Journal of Pharmacology
Volume	519
Issue number	1-2
DOIs	https://doi.org/10.1016/j.ejphar.2005.06.043
State	Published - 5 Sep 2005

Bibliographical note

Funding Information:
This study was supported by a grant from Korea Science and Engineering Foundation (KOSEF), and Ministry of Science and Technology (MOST), Korean government.

Keywords

Antioxidant enzyme
Reactive oxygen species
Tectorigenin

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10.1016/j.ejphar.2005.06.043

Cite this

Kang, K. A., Lee, K. H., Chae, S., Zhang, R., Jung, M. S., Kim, S. Y., Kim, H. S., Kim, D. H., & Hyun, J. W. (2005). Cytoprotective effect of tectorigenin, a metabolite formed by transformation of tectoridin by intestinal microflora, on oxidative stress induced by hydrogen peroxide. European Journal of Pharmacology, 519(1-2), 16-23. https://doi.org/10.1016/j.ejphar.2005.06.043

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title = "Cytoprotective effect of tectorigenin, a metabolite formed by transformation of tectoridin by intestinal microflora, on oxidative stress induced by hydrogen peroxide",

abstract = "In the present study, the antioxidative properties of tectorigenin, a metabolite formed by transformation of tectoridin by intestinal microflora, were investigated. Tectorigenin was found to scavenge intracellular reactive oxygen species, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, and thus prevented lipid peroxidation. The radical scavenging activity of tectorigenin protected the viability of Chinese hamster lung fibroblast (V79-4) cells exposed to hydrogen peroxide (H2O2) via activation of extracellular signal regulated kinase (ERK) pathway. Furthermore, tectorigenin reduced the apoptotic cells formation and cell cycle arrest at G2/M phase induced by H2O2. Tectorigenin increased the activities of cellular antioxidant enzymes like superoxide dismutase, catalase, glutathione peroxidase, and also increased their protein level. Taken together, these findings suggest that tectorigenin protected V79-4 cells against H 2O2 damage, by enhancing the antioxidative activity and by activating ERK pathway.",

keywords = "Antioxidant enzyme, Reactive oxygen species, Tectorigenin",

author = "Kang, {Kyoung Ah} and Lee, {Kyoung Hwa} and Sungwook Chae and Rui Zhang and Jung, {Myung Sun} and Kim, {So Young} and Kim, {Hee Sun} and Kim, {Dong Hyun} and Hyun, {Jin Won}",

note = "Funding Information: This study was supported by a grant from Korea Science and Engineering Foundation (KOSEF), and Ministry of Science and Technology (MOST), Korean government.",

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Cytoprotective effect of tectorigenin, a metabolite formed by transformation of tectoridin by intestinal microflora, on oxidative stress induced by hydrogen peroxide. / Kang, Kyoung Ah; Lee, Kyoung Hwa; Chae, Sungwook et al.
In: European Journal of Pharmacology, Vol. 519, No. 1-2, 05.09.2005, p. 16-23.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Cytoprotective effect of tectorigenin, a metabolite formed by transformation of tectoridin by intestinal microflora, on oxidative stress induced by hydrogen peroxide

AU - Kang, Kyoung Ah

AU - Lee, Kyoung Hwa

AU - Chae, Sungwook

AU - Zhang, Rui

AU - Jung, Myung Sun

AU - Kim, So Young

AU - Kim, Hee Sun

AU - Kim, Dong Hyun

AU - Hyun, Jin Won

N1 - Funding Information: This study was supported by a grant from Korea Science and Engineering Foundation (KOSEF), and Ministry of Science and Technology (MOST), Korean government.

PY - 2005/9/5

Y1 - 2005/9/5

N2 - In the present study, the antioxidative properties of tectorigenin, a metabolite formed by transformation of tectoridin by intestinal microflora, were investigated. Tectorigenin was found to scavenge intracellular reactive oxygen species, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, and thus prevented lipid peroxidation. The radical scavenging activity of tectorigenin protected the viability of Chinese hamster lung fibroblast (V79-4) cells exposed to hydrogen peroxide (H2O2) via activation of extracellular signal regulated kinase (ERK) pathway. Furthermore, tectorigenin reduced the apoptotic cells formation and cell cycle arrest at G2/M phase induced by H2O2. Tectorigenin increased the activities of cellular antioxidant enzymes like superoxide dismutase, catalase, glutathione peroxidase, and also increased their protein level. Taken together, these findings suggest that tectorigenin protected V79-4 cells against H 2O2 damage, by enhancing the antioxidative activity and by activating ERK pathway.

AB - In the present study, the antioxidative properties of tectorigenin, a metabolite formed by transformation of tectoridin by intestinal microflora, were investigated. Tectorigenin was found to scavenge intracellular reactive oxygen species, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, and thus prevented lipid peroxidation. The radical scavenging activity of tectorigenin protected the viability of Chinese hamster lung fibroblast (V79-4) cells exposed to hydrogen peroxide (H2O2) via activation of extracellular signal regulated kinase (ERK) pathway. Furthermore, tectorigenin reduced the apoptotic cells formation and cell cycle arrest at G2/M phase induced by H2O2. Tectorigenin increased the activities of cellular antioxidant enzymes like superoxide dismutase, catalase, glutathione peroxidase, and also increased their protein level. Taken together, these findings suggest that tectorigenin protected V79-4 cells against H 2O2 damage, by enhancing the antioxidative activity and by activating ERK pathway.

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KW - Reactive oxygen species

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ER -

Cytoprotective effect of tectorigenin, a metabolite formed by transformation of tectoridin by intestinal microflora, on oxidative stress induced by hydrogen peroxide

Abstract

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Keywords

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