Cytoprotective effect of tectorigenin, a metabolite formed by transformation of tectoridin by intestinal microflora, on oxidative stress induced by hydrogen peroxide

Kyoung Ah Kang, Kyoung Hwa Lee, Sungwook Chae, Rui Zhang, Myung Sun Jung, So Young Kim, Hee Sun Kim, Dong Hyun Kim, Jin Won Hyun

Research output: Contribution to journalArticlepeer-review

45 Scopus citations

Abstract

In the present study, the antioxidative properties of tectorigenin, a metabolite formed by transformation of tectoridin by intestinal microflora, were investigated. Tectorigenin was found to scavenge intracellular reactive oxygen species, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, and thus prevented lipid peroxidation. The radical scavenging activity of tectorigenin protected the viability of Chinese hamster lung fibroblast (V79-4) cells exposed to hydrogen peroxide (H2O2) via activation of extracellular signal regulated kinase (ERK) pathway. Furthermore, tectorigenin reduced the apoptotic cells formation and cell cycle arrest at G2/M phase induced by H2O2. Tectorigenin increased the activities of cellular antioxidant enzymes like superoxide dismutase, catalase, glutathione peroxidase, and also increased their protein level. Taken together, these findings suggest that tectorigenin protected V79-4 cells against H 2O2 damage, by enhancing the antioxidative activity and by activating ERK pathway.

Original languageEnglish
Pages (from-to)16-23
Number of pages8
JournalEuropean Journal of Pharmacology
Volume519
Issue number1-2
DOIs
StatePublished - 5 Sep 2005

Keywords

  • Antioxidant enzyme
  • Reactive oxygen species
  • Tectorigenin

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