Cytokine responses in mice infected with Clonorchis sinensis

Yang Kyu Choi, Byung Il Yoon, Young Suk Won, Chul Ho Lee, Byung Hwa Hyun, Hyoung Chin Kim, Goo Taeg Oh, Dae Yong Kim

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39 Scopus citations


FVB and BALB/c mice show different morbidity, development of Clonorchis sinensis, and pathological changes following C. sinensis infection. FVB mice are susceptible and BALB/c mice are relatively more resistant to C. sinensis infection. To investigate the relationship between cytokine reaction and susceptibility to C. sinensis infection in FVB and BALB/c mice, we described both the patterns and kinetics of Th1 cytokines and Th2 cytokines in spleen cell culture. Interleukin (IL)-4 and IL-10 cytokine production in the culture supernatants of the concanavalin-A-stimulated spleen cells increased at 2-3 weeks post-infection in both strains. IL-5 production increased between 2 and 5 weeks post-infection in both strains, and reached a peak level at 2 weeks post-infection in BALB/c mice and 4 weeks post-infection in FVB mice. In contrast, gamma interferon (IFN-γ) production decreased between 2 and 4 weeks in both strains. IL-2 production increased slightly in BALB/c mice following infection, but was unchanged in FVB mice. IL-4 production over preinfection levels was significantly higher in FVB mice, whereas IFN-γ, IL-2, and IL-10 production were significantly higher in BALB/c mice. The levels of serum immunoglobulin E (IgE) and blood eosinophils in both mouse strains significantly increased between 3 and 6 weeks postinfection. Serum IgE levels were significantly higher in FVB mice than in BALB/c mice. The results of this study suggest that susceptibility to C. sinensis infection is associated with Th2 cytokine production, especially IL-4 which is predominant in relatively susceptible FVB mice.

Original languageEnglish
Pages (from-to)87-93
Number of pages7
JournalParasitology Research
Issue number2
StatePublished - 1 Oct 2003

Bibliographical note

Funding Information:
Acknowledgements We thank Dr. Todd Reinhart, Department of Infectious Diseases and Microbiology, University of Pittsburgh, USA for helpful comments on the manuscript. This work was supported by grants (KBS0120313 and BDM0300211) from the Ministry of Science and Technology of Korea. This work was also partially supported by the Brain Korea 21 Project.


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