Cyclohexanone-induced stress metabolism of Escherichia coli and Corynebacterium glutamicum

Na Rae Lee, Ji Yeong Yun, Sun Mee Lee, Jin Byung Park

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


Solvent stress occurs during whole-cell biocatalysis of organic chemicals. Organic substrates and/or products may accumulate in the cellular membranes of whole cells, causing structural destabilization of the membranes, which leads to disturbances in cellular carbon and energy metabolism. Here, we investigate the effect of cyclohexanone on carbon metabolism in Escherichia coli BL21 and Corynebacterium glutamicum ATCC13032. Adding cyclohexanone to the culture medium (i.e., glucose mineral medium) resulted in a decreased specific growth rate and increased cellular maintenance energy in both strains of bacteria. Notably, carbon metabolism, which is mainly involved to increase cellular maintenance energy, was very different between the bacteria. Carbon flux into the acetic acid fermentation pathway was dominantly enhanced in E. coli, whereas the TCA cycle appeared to be activated in C. glutamicum. In fact, carbon flux into the TCA cycle in E. coli appeared to be reduced with increasing amounts of cyclohexanone in the culture medium. Metabolic engineering of E. coli cells to maintain or improve TCA cycle activity and, presumably, that of the electron transport chain, which are involved in regeneration of cofactors (e.g., NAD(P)H and ATP) and formation of toxic metabolites (e.g., acetic acid), may be useful in increasing solvent tolerance and biotransformation of organic chemicals (e.g., cyclohexanone).

Original languageEnglish
Pages (from-to)1088-1098
Number of pages11
JournalBiotechnology and Bioprocess Engineering
Issue number6
StatePublished - 1 Nov 2015

Bibliographical note

Funding Information:
This study was supported by a Marine Biomaterials Research Center grant from the Marine Biotechnology Program funded by the Ministry of Oceans and Fisheries, Korea.

Publisher Copyright:
© 2015, The Korean Society for Biotechnology and Bioengineering and Springer-Verlag Berlin Heidelberg.


  • constraints-based flux analysis
  • Corynebacterium glutamicum
  • cyclohexanone
  • Escherichia coli
  • solvent stress


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