Curcumin suppresses the TPA-induced invasion through inhibition of PKCα-dependent MMP-expression in MCF-7 human breast cancer cells

Jeong Mi Kim, Eun Mi Noh, Kang Beam Kwon, Jong Suk Kim, Yong Ouk You, Jin Ki Hwang, Bo Mi Hwang, Byeong Soo Kim, Sung Hoo Lee, Seung Jin Lee, Sung Hoo Jung, Hyun Jo Youn, Young Rae Lee

Research output: Contribution to journalArticlepeer-review

65 Scopus citations

Abstract

Curcumin (diferuloylmethane) is a polyphenol derived from the plant turmeric (Curcuma longa), which is commonly used as a spice. Although anti-carcinogenic, anti-oxidant, anti-inflammation, and anti-angiogenic properties have been reported, the effect of curcumin on breast cancer metastasis is unknown. Matrix metalloproteinase-9 (MMP-9) is a major component in cancer cell invasion. In this study, we investigated the inhibitory effect of curcumin on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced MMP-9 expression and cell invasion and the molecular mechanisms involved in MCF-7 cells. Our results showed that curcumin inhibits TPA-induced MMP-9 expression and cell invasion through suppressing NF-κB and AP-1 activation. Also, curcumin strongly repressed the TPA-induced phosphorylation of p38 and JNK and inhibited TPA-induced translocation of PKCα from the cytosol to the membrane, but did not affect the translocation of PKCδ. These results indicate that curcumin-mediated inhibition of TPA-induced MMP-9 expression and cell invasion involves the suppression of the PKCα, MAPK and NF-κB/AP-1 pathway in MCF-7 cells. Curcumin may have potential value in restricting breast cancer metastasis.

Original languageEnglish
Pages (from-to)1085-1092
Number of pages8
JournalPhytomedicine
Volume19
Issue number12
DOIs
StatePublished - 15 Sep 2012

Keywords

  • Curcumin
  • Invasion
  • MCF-7
  • MMP-9
  • PKCα

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