Curcumin suppresses phorbol ester-induced matrix metalloproteinase-9 expression by inhibiting the PKC to MAPK signaling pathways in human astroglioma cells

Moon Sook Woo; Soo Hyun Jung; So Young Kim; Jin Won Hyun; Kwang Ho Ko; Won Ki Kim; Hee Sun Kim

doi:10.1016/j.bbrc.2005.07.174

Curcumin suppresses phorbol ester-induced matrix metalloproteinase-9 expression by inhibiting the PKC to MAPK signaling pathways in human astroglioma cells

Moon Sook Woo
, Soo Hyun Jung
, So Young Kim
, Jin Won Hyun
, Kwang Ho Ko
, Won Ki Kim
, Hee Sun Kim

Department of Molecular Medicine

Research output: Contribution to journal › Article › peer-review

171 Scopus citations

Abstract

The aberrant expression of matrix metalloproteinase-9 (MMP-9) is implicated in the invasion and angiogenesis process of brain tumor. This study has investigated the effects of curcumin on MMP-9 expression in human astroglioma cell lines. Curcumin significantly inhibited the MMP-9 enzymatic activity and protein expression that was induced by PMA. The inhibitory effect of curcumin on MMP-9 expression correlates with the decreased MMP-9 mRNA level and the suppression of MMP-9 promoter activity. The curcumin-mediated inhibition of MMP-9 gene expression appears to occur via NF-κB and AP-1 because their DNA binding activities were suppressed by curcumin. Furthermore, curcumin strongly repressed the PMA-induced phosphorylation of ERK, JNK, and p38 MAP kinase, which were dependent on the PKC pathway. Therefore, the inhibition of MMP-9 expression by curcumin might have therapeutic potential for controlling the growth and invasiveness of brain tumor.

Original language	English
Pages (from-to)	1017-1025
Number of pages	9
Journal	Biochemical and Biophysical Research Communications
Volume	335
Issue number	4
DOIs	https://doi.org/10.1016/j.bbrc.2005.07.174
State	Published - 7 Oct 2005

Bibliographical note

Funding Information:
This work was supported by a grant to H.S. Kim (02-PJ2-PG10-21403-0001) from the Korea Health 21 R&D Project, Ministry of Health and Welfare, Republic of Korea, and in part by a grant from Neurobiology Research Program (M10412000042-04N1200-04210) of the Ministry of Science and Technology, Republic of Korea.

Keywords

AP-1
Astroglioma
Curcumin
Gene expression
MAP kinase
Matrix metalloproteinase-9
NF-κB
PKC
Signal transduction

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10.1016/j.bbrc.2005.07.174

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title = "Curcumin suppresses phorbol ester-induced matrix metalloproteinase-9 expression by inhibiting the PKC to MAPK signaling pathways in human astroglioma cells",

abstract = "The aberrant expression of matrix metalloproteinase-9 (MMP-9) is implicated in the invasion and angiogenesis process of brain tumor. This study has investigated the effects of curcumin on MMP-9 expression in human astroglioma cell lines. Curcumin significantly inhibited the MMP-9 enzymatic activity and protein expression that was induced by PMA. The inhibitory effect of curcumin on MMP-9 expression correlates with the decreased MMP-9 mRNA level and the suppression of MMP-9 promoter activity. The curcumin-mediated inhibition of MMP-9 gene expression appears to occur via NF-κB and AP-1 because their DNA binding activities were suppressed by curcumin. Furthermore, curcumin strongly repressed the PMA-induced phosphorylation of ERK, JNK, and p38 MAP kinase, which were dependent on the PKC pathway. Therefore, the inhibition of MMP-9 expression by curcumin might have therapeutic potential for controlling the growth and invasiveness of brain tumor.",

keywords = "AP-1, Astroglioma, Curcumin, Gene expression, MAP kinase, Matrix metalloproteinase-9, NF-κB, PKC, Signal transduction",

author = "Woo, \{Moon Sook\} and Jung, \{Soo Hyun\} and Kim, \{So Young\} and Hyun, \{Jin Won\} and Ko, \{Kwang Ho\} and Kim, \{Won Ki\} and Kim, \{Hee Sun\}",

note = "Funding Information: This work was supported by a grant to H.S. Kim (02-PJ2-PG10-21403-0001) from the Korea Health 21 R\&D Project, Ministry of Health and Welfare, Republic of Korea, and in part by a grant from Neurobiology Research Program (M10412000042-04N1200-04210) of the Ministry of Science and Technology, Republic of Korea.",

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Curcumin suppresses phorbol ester-induced matrix metalloproteinase-9 expression by inhibiting the PKC to MAPK signaling pathways in human astroglioma cells. / Woo, Moon Sook; Jung, Soo Hyun; Kim, So Young et al.
In: Biochemical and Biophysical Research Communications, Vol. 335, No. 4, 07.10.2005, p. 1017-1025.

Research output: Contribution to journal › Article › peer-review

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AU - Jung, Soo Hyun

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AU - Hyun, Jin Won

AU - Ko, Kwang Ho

AU - Kim, Won Ki

AU - Kim, Hee Sun

N1 - Funding Information: This work was supported by a grant to H.S. Kim (02-PJ2-PG10-21403-0001) from the Korea Health 21 R&D Project, Ministry of Health and Welfare, Republic of Korea, and in part by a grant from Neurobiology Research Program (M10412000042-04N1200-04210) of the Ministry of Science and Technology, Republic of Korea.

PY - 2005/10/7

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N2 - The aberrant expression of matrix metalloproteinase-9 (MMP-9) is implicated in the invasion and angiogenesis process of brain tumor. This study has investigated the effects of curcumin on MMP-9 expression in human astroglioma cell lines. Curcumin significantly inhibited the MMP-9 enzymatic activity and protein expression that was induced by PMA. The inhibitory effect of curcumin on MMP-9 expression correlates with the decreased MMP-9 mRNA level and the suppression of MMP-9 promoter activity. The curcumin-mediated inhibition of MMP-9 gene expression appears to occur via NF-κB and AP-1 because their DNA binding activities were suppressed by curcumin. Furthermore, curcumin strongly repressed the PMA-induced phosphorylation of ERK, JNK, and p38 MAP kinase, which were dependent on the PKC pathway. Therefore, the inhibition of MMP-9 expression by curcumin might have therapeutic potential for controlling the growth and invasiveness of brain tumor.

AB - The aberrant expression of matrix metalloproteinase-9 (MMP-9) is implicated in the invasion and angiogenesis process of brain tumor. This study has investigated the effects of curcumin on MMP-9 expression in human astroglioma cell lines. Curcumin significantly inhibited the MMP-9 enzymatic activity and protein expression that was induced by PMA. The inhibitory effect of curcumin on MMP-9 expression correlates with the decreased MMP-9 mRNA level and the suppression of MMP-9 promoter activity. The curcumin-mediated inhibition of MMP-9 gene expression appears to occur via NF-κB and AP-1 because their DNA binding activities were suppressed by curcumin. Furthermore, curcumin strongly repressed the PMA-induced phosphorylation of ERK, JNK, and p38 MAP kinase, which were dependent on the PKC pathway. Therefore, the inhibition of MMP-9 expression by curcumin might have therapeutic potential for controlling the growth and invasiveness of brain tumor.

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KW - Curcumin

KW - Gene expression

KW - MAP kinase

KW - Matrix metalloproteinase-9

KW - NF-κB

KW - PKC

KW - Signal transduction

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ER -

Curcumin suppresses phorbol ester-induced matrix metalloproteinase-9 expression by inhibiting the PKC to MAPK signaling pathways in human astroglioma cells

Abstract

Bibliographical note

Keywords

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