Curcumin suppresses phorbol ester-induced matrix metalloproteinase-9 expression by inhibiting the PKC to MAPK signaling pathways in human astroglioma cells

Moon Sook Woo; Soo Hyun Jung; So Young Kim; Jin Won Hyun; Kwang Ho Ko; Won Ki Kim; Hee Sun Kim

doi:10.1016/j.bbrc.2005.07.174

Curcumin suppresses phorbol ester-induced matrix metalloproteinase-9 expression by inhibiting the PKC to MAPK signaling pathways in human astroglioma cells

Moon Sook Woo, Soo Hyun Jung, So Young Kim, Jin Won Hyun, Kwang Ho Ko, Won Ki Kim, Hee Sun Kim

Department of Molecular Medicine

Research output: Contribution to journal › Article › peer-review

169 Scopus citations

Abstract

The aberrant expression of matrix metalloproteinase-9 (MMP-9) is implicated in the invasion and angiogenesis process of brain tumor. This study has investigated the effects of curcumin on MMP-9 expression in human astroglioma cell lines. Curcumin significantly inhibited the MMP-9 enzymatic activity and protein expression that was induced by PMA. The inhibitory effect of curcumin on MMP-9 expression correlates with the decreased MMP-9 mRNA level and the suppression of MMP-9 promoter activity. The curcumin-mediated inhibition of MMP-9 gene expression appears to occur via NF-κB and AP-1 because their DNA binding activities were suppressed by curcumin. Furthermore, curcumin strongly repressed the PMA-induced phosphorylation of ERK, JNK, and p38 MAP kinase, which were dependent on the PKC pathway. Therefore, the inhibition of MMP-9 expression by curcumin might have therapeutic potential for controlling the growth and invasiveness of brain tumor.

Original language	English
Pages (from-to)	1017-1025
Number of pages	9
Journal	Biochemical and Biophysical Research Communications
Volume	335
Issue number	4
DOIs	https://doi.org/10.1016/j.bbrc.2005.07.174
State	Published - 7 Oct 2005

Bibliographical note

Funding Information:
This work was supported by a grant to H.S. Kim (02-PJ2-PG10-21403-0001) from the Korea Health 21 R&D Project, Ministry of Health and Welfare, Republic of Korea, and in part by a grant from Neurobiology Research Program (M10412000042-04N1200-04210) of the Ministry of Science and Technology, Republic of Korea.

Keywords

AP-1
Astroglioma
Curcumin
Gene expression
MAP kinase
Matrix metalloproteinase-9
NF-κB
PKC
Signal transduction

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10.1016/j.bbrc.2005.07.174

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title = "Curcumin suppresses phorbol ester-induced matrix metalloproteinase-9 expression by inhibiting the PKC to MAPK signaling pathways in human astroglioma cells",

abstract = "The aberrant expression of matrix metalloproteinase-9 (MMP-9) is implicated in the invasion and angiogenesis process of brain tumor. This study has investigated the effects of curcumin on MMP-9 expression in human astroglioma cell lines. Curcumin significantly inhibited the MMP-9 enzymatic activity and protein expression that was induced by PMA. The inhibitory effect of curcumin on MMP-9 expression correlates with the decreased MMP-9 mRNA level and the suppression of MMP-9 promoter activity. The curcumin-mediated inhibition of MMP-9 gene expression appears to occur via NF-κB and AP-1 because their DNA binding activities were suppressed by curcumin. Furthermore, curcumin strongly repressed the PMA-induced phosphorylation of ERK, JNK, and p38 MAP kinase, which were dependent on the PKC pathway. Therefore, the inhibition of MMP-9 expression by curcumin might have therapeutic potential for controlling the growth and invasiveness of brain tumor.",

keywords = "AP-1, Astroglioma, Curcumin, Gene expression, MAP kinase, Matrix metalloproteinase-9, NF-κB, PKC, Signal transduction",

author = "Woo, {Moon Sook} and Jung, {Soo Hyun} and Kim, {So Young} and Hyun, {Jin Won} and Ko, {Kwang Ho} and Kim, {Won Ki} and Kim, {Hee Sun}",

note = "Funding Information: This work was supported by a grant to H.S. Kim (02-PJ2-PG10-21403-0001) from the Korea Health 21 R&D Project, Ministry of Health and Welfare, Republic of Korea, and in part by a grant from Neurobiology Research Program (M10412000042-04N1200-04210) of the Ministry of Science and Technology, Republic of Korea.",

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Curcumin suppresses phorbol ester-induced matrix metalloproteinase-9 expression by inhibiting the PKC to MAPK signaling pathways in human astroglioma cells. / Woo, Moon Sook; Jung, Soo Hyun; Kim, So Young et al.
In: Biochemical and Biophysical Research Communications, Vol. 335, No. 4, 07.10.2005, p. 1017-1025.

Research output: Contribution to journal › Article › peer-review

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AU - Hyun, Jin Won

AU - Ko, Kwang Ho

AU - Kim, Won Ki

AU - Kim, Hee Sun

N1 - Funding Information: This work was supported by a grant to H.S. Kim (02-PJ2-PG10-21403-0001) from the Korea Health 21 R&D Project, Ministry of Health and Welfare, Republic of Korea, and in part by a grant from Neurobiology Research Program (M10412000042-04N1200-04210) of the Ministry of Science and Technology, Republic of Korea.

PY - 2005/10/7

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N2 - The aberrant expression of matrix metalloproteinase-9 (MMP-9) is implicated in the invasion and angiogenesis process of brain tumor. This study has investigated the effects of curcumin on MMP-9 expression in human astroglioma cell lines. Curcumin significantly inhibited the MMP-9 enzymatic activity and protein expression that was induced by PMA. The inhibitory effect of curcumin on MMP-9 expression correlates with the decreased MMP-9 mRNA level and the suppression of MMP-9 promoter activity. The curcumin-mediated inhibition of MMP-9 gene expression appears to occur via NF-κB and AP-1 because their DNA binding activities were suppressed by curcumin. Furthermore, curcumin strongly repressed the PMA-induced phosphorylation of ERK, JNK, and p38 MAP kinase, which were dependent on the PKC pathway. Therefore, the inhibition of MMP-9 expression by curcumin might have therapeutic potential for controlling the growth and invasiveness of brain tumor.

AB - The aberrant expression of matrix metalloproteinase-9 (MMP-9) is implicated in the invasion and angiogenesis process of brain tumor. This study has investigated the effects of curcumin on MMP-9 expression in human astroglioma cell lines. Curcumin significantly inhibited the MMP-9 enzymatic activity and protein expression that was induced by PMA. The inhibitory effect of curcumin on MMP-9 expression correlates with the decreased MMP-9 mRNA level and the suppression of MMP-9 promoter activity. The curcumin-mediated inhibition of MMP-9 gene expression appears to occur via NF-κB and AP-1 because their DNA binding activities were suppressed by curcumin. Furthermore, curcumin strongly repressed the PMA-induced phosphorylation of ERK, JNK, and p38 MAP kinase, which were dependent on the PKC pathway. Therefore, the inhibition of MMP-9 expression by curcumin might have therapeutic potential for controlling the growth and invasiveness of brain tumor.

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Curcumin suppresses phorbol ester-induced matrix metalloproteinase-9 expression by inhibiting the PKC to MAPK signaling pathways in human astroglioma cells

Abstract

Bibliographical note

Keywords

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