Cultivation of Lactobacillus crispatus KLB46 isolated from human vagina

Chung Eun Chang, Seung Cheol Kim, Jae Seong So, Hyun Shik Yun

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Bacterial vaginosis can be treated by restoring the normal vaginal flora using lactobacilli. Lactobacillus crispatus KLB46 that was isolated from the human vagina has a strong antimicrobial activity and was grown in a batch and in a continuous fermentor. During batch cultivation, the maximum specific growth rate of L. crispatus KLB 46 was 0.63 h-1 and the highest viable cell count (1.9 × 109 CFU/mL) was obtained at pH 5.5. L. crispatus KLB 46 did not grow well at either pH 3.5 or 7.5. During continuous cultivation, the highest viable cell count (1.53 × 109 CFU/mL) was obtained at a dilution rate of 0.32 h-1. However, the maximum productivity of viable cells was obtained at a dilution rate of 0.52 h-1, and was 7.33 × 1011 CFU L-1 h-1, that is approximately 5 times higher than that obtained from batch culture.

Original languageEnglish
Pages (from-to)128-132
Number of pages5
JournalBiotechnology and Bioprocess Engineering
Volume6
Issue number2
DOIs
StatePublished - 2001

Keywords

  • Bacterial vaginosis
  • Cultivation
  • Lactobacillus crispatus
  • Viable cell count

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