Cultivation of Lactobacillus crispatus KLB46 isolated from human vagina

Chung Eun Chang; Seung Cheol Kim; Jae Seong So; Hyun Shik Yun

doi:10.1007/BF02931958

Cultivation of Lactobacillus crispatus KLB46 isolated from human vagina

Chung Eun Chang, Seung Cheol Kim, Jae Seong So, Hyun Shik Yun

Department of Obstetrics and Gynecology

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Bacterial vaginosis can be treated by restoring the normal vaginal flora using lactobacilli. Lactobacillus crispatus KLB46 that was isolated from the human vagina has a strong antimicrobial activity and was grown in a batch and in a continuous fermentor. During batch cultivation, the maximum specific growth rate of L. crispatus KLB 46 was 0.63 h^-1 and the highest viable cell count (1.9 × 10⁹ CFU/mL) was obtained at pH 5.5. L. crispatus KLB 46 did not grow well at either pH 3.5 or 7.5. During continuous cultivation, the highest viable cell count (1.53 × 10⁹ CFU/mL) was obtained at a dilution rate of 0.32 h^-1. However, the maximum productivity of viable cells was obtained at a dilution rate of 0.52 h^-1, and was 7.33 × 10¹¹ CFU L^-1 h^-1, that is approximately 5 times higher than that obtained from batch culture.

Original language	English
Pages (from-to)	128-132
Number of pages	5
Journal	Biotechnology and Bioprocess Engineering
Volume	6
Issue number	2
DOIs	https://doi.org/10.1007/BF02931958
State	Published - 2001

Bibliographical note

Funding Information:
edge the financial support of the Korea Research Foundation (1998-020-E00060) made in the program year of 1998.

Keywords

Bacterial vaginosis
Cultivation
Lactobacillus crispatus
Viable cell count

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10.1007/BF02931958

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@article{115de44217d04373a7a4701b3a1cbd60,

title = "Cultivation of Lactobacillus crispatus KLB46 isolated from human vagina",

abstract = "Bacterial vaginosis can be treated by restoring the normal vaginal flora using lactobacilli. Lactobacillus crispatus KLB46 that was isolated from the human vagina has a strong antimicrobial activity and was grown in a batch and in a continuous fermentor. During batch cultivation, the maximum specific growth rate of L. crispatus KLB 46 was 0.63 h-1 and the highest viable cell count (1.9 × 109 CFU/mL) was obtained at pH 5.5. L. crispatus KLB 46 did not grow well at either pH 3.5 or 7.5. During continuous cultivation, the highest viable cell count (1.53 × 109 CFU/mL) was obtained at a dilution rate of 0.32 h-1. However, the maximum productivity of viable cells was obtained at a dilution rate of 0.52 h-1, and was 7.33 × 1011 CFU L-1 h-1, that is approximately 5 times higher than that obtained from batch culture.",

keywords = "Bacterial vaginosis, Cultivation, Lactobacillus crispatus, Viable cell count",

author = "Chang, {Chung Eun} and Kim, {Seung Cheol} and So, {Jae Seong} and Yun, {Hyun Shik}",

note = "Funding Information: edge the financial support of the Korea Research Foundation (1998-020-E00060) made in the program year of 1998.",

year = "2001",

doi = "10.1007/BF02931958",

language = "English",

volume = "6",

pages = "128--132",

journal = "Biotechnology and Bioprocess Engineering",

issn = "1226-8372",

publisher = "Korean Society for Biotechnology and Bioengineering",

number = "2",

}

TY - JOUR

T1 - Cultivation of Lactobacillus crispatus KLB46 isolated from human vagina

AU - Chang, Chung Eun

AU - Kim, Seung Cheol

AU - So, Jae Seong

AU - Yun, Hyun Shik

N1 - Funding Information: edge the financial support of the Korea Research Foundation (1998-020-E00060) made in the program year of 1998.

PY - 2001

Y1 - 2001

N2 - Bacterial vaginosis can be treated by restoring the normal vaginal flora using lactobacilli. Lactobacillus crispatus KLB46 that was isolated from the human vagina has a strong antimicrobial activity and was grown in a batch and in a continuous fermentor. During batch cultivation, the maximum specific growth rate of L. crispatus KLB 46 was 0.63 h-1 and the highest viable cell count (1.9 × 109 CFU/mL) was obtained at pH 5.5. L. crispatus KLB 46 did not grow well at either pH 3.5 or 7.5. During continuous cultivation, the highest viable cell count (1.53 × 109 CFU/mL) was obtained at a dilution rate of 0.32 h-1. However, the maximum productivity of viable cells was obtained at a dilution rate of 0.52 h-1, and was 7.33 × 1011 CFU L-1 h-1, that is approximately 5 times higher than that obtained from batch culture.

AB - Bacterial vaginosis can be treated by restoring the normal vaginal flora using lactobacilli. Lactobacillus crispatus KLB46 that was isolated from the human vagina has a strong antimicrobial activity and was grown in a batch and in a continuous fermentor. During batch cultivation, the maximum specific growth rate of L. crispatus KLB 46 was 0.63 h-1 and the highest viable cell count (1.9 × 109 CFU/mL) was obtained at pH 5.5. L. crispatus KLB 46 did not grow well at either pH 3.5 or 7.5. During continuous cultivation, the highest viable cell count (1.53 × 109 CFU/mL) was obtained at a dilution rate of 0.32 h-1. However, the maximum productivity of viable cells was obtained at a dilution rate of 0.52 h-1, and was 7.33 × 1011 CFU L-1 h-1, that is approximately 5 times higher than that obtained from batch culture.

KW - Bacterial vaginosis

KW - Cultivation

KW - Lactobacillus crispatus

KW - Viable cell count

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U2 - 10.1007/BF02931958

DO - 10.1007/BF02931958

M3 - Article

AN - SCOPUS:0001052017

SN - 1226-8372

VL - 6

SP - 128

EP - 132

JO - Biotechnology and Bioprocess Engineering

JF - Biotechnology and Bioprocess Engineering

IS - 2

ER -

Cultivation of Lactobacillus crispatus KLB46 isolated from human vagina

Abstract

Bibliographical note

Keywords

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