Crystallization and preliminary X-ray crystallographic analysis of a yedU gene product from Escherichia coli

Ok Gwan Kim; In Kwon Kim; Ghyung Hwa Kim; Junsang Ko; Chankyu Park; Pann Ghill Suh; Sa Ouk Kang; Heung Soo Lee; Sun Shin Cha

doi:10.1107/S0907444902007369

Crystallization and preliminary X-ray crystallographic analysis of a yedU gene product from Escherichia coli

Ok Gwan Kim
, In Kwon Kim
, Ghyung Hwa Kim
, Junsang Ko
, Chankyu Park
, Pann Ghill Suh
, Sa Ouk Kang
, Heung Soo Lee
, Sun Shin Cha

Department of Chemistry & Nanoscience

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

A yedU gene product with a molecular mass of 31 kDa is a hypothetical protein with no known function. The protein was purified and crystallized at 296 K. X-ray diffraction data have been collected to 2.3 Å using synchrotron radiation. The crystals belong to the primitive orthorhombic system, with unit-cell parameters a = 50.56, b = 63.45, c = 168.02 Å. The asymmetric unit contains two monomers of the protein, with a corresponding V_M of 2.25 Å³ Da^-1 and a solvent content of 44.84%.

Original language	English
Pages (from-to)	1217-1219
Number of pages	3
Journal	Acta Crystallographica Section D: Biological Crystallography
Volume	58
Issue number	7
DOIs	https://doi.org/10.1107/S0907444902007369
State	Published - 2002

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title = "Crystallization and preliminary X-ray crystallographic analysis of a yedU gene product from Escherichia coli",

abstract = "A yedU gene product with a molecular mass of 31 kDa is a hypothetical protein with no known function. The protein was purified and crystallized at 296 K. X-ray diffraction data have been collected to 2.3 {\AA} using synchrotron radiation. The crystals belong to the primitive orthorhombic system, with unit-cell parameters a = 50.56, b = 63.45, c = 168.02 {\AA}. The asymmetric unit contains two monomers of the protein, with a corresponding VM of 2.25 {\AA}3 Da-1 and a solvent content of 44.84\%.",

author = "Kim, \{Ok Gwan\} and Kim, \{In Kwon\} and Kim, \{Ghyung Hwa\} and Junsang Ko and Chankyu Park and Suh, \{Pann Ghill\} and Kang, \{Sa Ouk\} and Lee, \{Heung Soo\} and Cha, \{Sun Shin\}",

year = "2002",

doi = "10.1107/S0907444902007369",

language = "English",

volume = "58",

pages = "1217--1219",

journal = "Acta Crystallographica Section D: Biological Crystallography",

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T1 - Crystallization and preliminary X-ray crystallographic analysis of a yedU gene product from Escherichia coli

AU - Kim, Ok Gwan

AU - Kim, In Kwon

AU - Kim, Ghyung Hwa

AU - Ko, Junsang

AU - Park, Chankyu

AU - Suh, Pann Ghill

AU - Kang, Sa Ouk

AU - Lee, Heung Soo

AU - Cha, Sun Shin

PY - 2002

Y1 - 2002

N2 - A yedU gene product with a molecular mass of 31 kDa is a hypothetical protein with no known function. The protein was purified and crystallized at 296 K. X-ray diffraction data have been collected to 2.3 Å using synchrotron radiation. The crystals belong to the primitive orthorhombic system, with unit-cell parameters a = 50.56, b = 63.45, c = 168.02 Å. The asymmetric unit contains two monomers of the protein, with a corresponding VM of 2.25 Å3 Da-1 and a solvent content of 44.84%.

AB - A yedU gene product with a molecular mass of 31 kDa is a hypothetical protein with no known function. The protein was purified and crystallized at 296 K. X-ray diffraction data have been collected to 2.3 Å using synchrotron radiation. The crystals belong to the primitive orthorhombic system, with unit-cell parameters a = 50.56, b = 63.45, c = 168.02 Å. The asymmetric unit contains two monomers of the protein, with a corresponding VM of 2.25 Å3 Da-1 and a solvent content of 44.84%.

UR - https://www.scopus.com/pages/publications/0035997142

U2 - 10.1107/S0907444902007369

DO - 10.1107/S0907444902007369

M3 - Article

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AN - SCOPUS:0035997142

SN - 0907-4449

VL - 58

SP - 1217

EP - 1219

JO - Acta Crystallographica Section D: Biological Crystallography

JF - Acta Crystallographica Section D: Biological Crystallography

IS - 7

ER -

Crystallization and preliminary X-ray crystallographic analysis of a yedU gene product from Escherichia coli

Abstract

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