Crystallization and preliminary X-ray crystallographic analysis of a yedU gene product from Escherichia coli

Ok Gwan Kim; In Kwon Kim; Ghyung Hwa Kim; Junsang Ko; Chankyu Park; Pann Ghill Suh; Sa Ouk Kang; Heung Soo Lee; Sun Shin Cha

doi:10.1107/S0907444902007369

Crystallization and preliminary X-ray crystallographic analysis of a yedU gene product from Escherichia coli

Ok Gwan Kim, In Kwon Kim, Ghyung Hwa Kim, Junsang Ko, Chankyu Park, Pann Ghill Suh, Sa Ouk Kang, Heung Soo Lee, Sun Shin Cha

Chemistry & Nanoscience

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

A yedU gene product with a molecular mass of 31 kDa is a hypothetical protein with no known function. The protein was purified and crystallized at 296 K. X-ray diffraction data have been collected to 2.3 Å using synchrotron radiation. The crystals belong to the primitive orthorhombic system, with unit-cell parameters a = 50.56, b = 63.45, c = 168.02 Å. The asymmetric unit contains two monomers of the protein, with a corresponding V_M of 2.25 Å³ Da^-1 and a solvent content of 44.84%.

Original language	English
Pages (from-to)	1217-1219
Number of pages	3
Journal	Acta Crystallographica Section D: Biological Crystallography
Volume	58
Issue number	7
DOIs	https://doi.org/10.1107/S0907444902007369
State	Published - 2002

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title = "Crystallization and preliminary X-ray crystallographic analysis of a yedU gene product from Escherichia coli",

abstract = "A yedU gene product with a molecular mass of 31 kDa is a hypothetical protein with no known function. The protein was purified and crystallized at 296 K. X-ray diffraction data have been collected to 2.3 {\AA} using synchrotron radiation. The crystals belong to the primitive orthorhombic system, with unit-cell parameters a = 50.56, b = 63.45, c = 168.02 {\AA}. The asymmetric unit contains two monomers of the protein, with a corresponding VM of 2.25 {\AA}3 Da-1 and a solvent content of 44.84%.",

author = "Kim, {Ok Gwan} and Kim, {In Kwon} and Kim, {Ghyung Hwa} and Junsang Ko and Chankyu Park and Suh, {Pann Ghill} and Kang, {Sa Ouk} and Lee, {Heung Soo} and Cha, {Sun Shin}",

year = "2002",

doi = "10.1107/S0907444902007369",

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T1 - Crystallization and preliminary X-ray crystallographic analysis of a yedU gene product from Escherichia coli

AU - Kim, Ok Gwan

AU - Kim, In Kwon

AU - Kim, Ghyung Hwa

AU - Ko, Junsang

AU - Park, Chankyu

AU - Suh, Pann Ghill

AU - Kang, Sa Ouk

AU - Lee, Heung Soo

AU - Cha, Sun Shin

PY - 2002

Y1 - 2002

N2 - A yedU gene product with a molecular mass of 31 kDa is a hypothetical protein with no known function. The protein was purified and crystallized at 296 K. X-ray diffraction data have been collected to 2.3 Å using synchrotron radiation. The crystals belong to the primitive orthorhombic system, with unit-cell parameters a = 50.56, b = 63.45, c = 168.02 Å. The asymmetric unit contains two monomers of the protein, with a corresponding VM of 2.25 Å3 Da-1 and a solvent content of 44.84%.

AB - A yedU gene product with a molecular mass of 31 kDa is a hypothetical protein with no known function. The protein was purified and crystallized at 296 K. X-ray diffraction data have been collected to 2.3 Å using synchrotron radiation. The crystals belong to the primitive orthorhombic system, with unit-cell parameters a = 50.56, b = 63.45, c = 168.02 Å. The asymmetric unit contains two monomers of the protein, with a corresponding VM of 2.25 Å3 Da-1 and a solvent content of 44.84%.

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DO - 10.1107/S0907444902007369

M3 - Article

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AN - SCOPUS:0035997142

SN - 0907-4449

VL - 58

SP - 1217

EP - 1219

JO - Acta Crystallographica Section D: Biological Crystallography

JF - Acta Crystallographica Section D: Biological Crystallography

IS - 7

ER -

Crystallization and preliminary X-ray crystallographic analysis of a yedU gene product from Escherichia coli

Abstract

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