Crystallization and preliminary X-ray crystallographic analysis of a ytfG gene product from Escherichia coli

In Kwon Kim; Hyung Soon Yim; Dong Won Kim; Young Min Kim; Heung Soo Lee; Sun Shin Cha; Sa Ouk Kang

doi:10.1107/S0907444903027781

Crystallization and preliminary X-ray crystallographic analysis of a ytfG gene product from Escherichia coli

In Kwon Kim, Hyung Soon Yim, Dong Won Kim, Young Min Kim, Heung Soo Lee, Sun Shin Cha, Sa Ouk Kang

Chemistry & Nanoscience

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

The Escherichia coli ytfG gene product, with NAD(P)H:quinone oxidoreductase activity, was crystallized by the hanging-drop vapour-diffusion method at 296 K. A 1.78 Å data set has been collected using synchrotron radiation at Pohang Light Source, South Korea. The crystal belongs to the primitive trigonal system, with unit-cell parameters a = b = 81.7, c = 76.8 Å. Analysis of the packing density shows that the asymmetric unit probably contains one monomer, with a solvent content of 48.8%.

Original language	English
Pages (from-to)	365-367
Number of pages	3
Journal	Acta Crystallographica Section D: Biological Crystallography
Volume	60
Issue number	2
DOIs	https://doi.org/10.1107/S0907444903027781
State	Published - Feb 2004

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title = "Crystallization and preliminary X-ray crystallographic analysis of a ytfG gene product from Escherichia coli",

abstract = "The Escherichia coli ytfG gene product, with NAD(P)H:quinone oxidoreductase activity, was crystallized by the hanging-drop vapour-diffusion method at 296 K. A 1.78 {\AA} data set has been collected using synchrotron radiation at Pohang Light Source, South Korea. The crystal belongs to the primitive trigonal system, with unit-cell parameters a = b = 81.7, c = 76.8 {\AA}. Analysis of the packing density shows that the asymmetric unit probably contains one monomer, with a solvent content of 48.8%.",

author = "Kim, {In Kwon} and Yim, {Hyung Soon} and Kim, {Dong Won} and Kim, {Young Min} and Lee, {Heung Soo} and Cha, {Sun Shin} and Kang, {Sa Ouk}",

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T1 - Crystallization and preliminary X-ray crystallographic analysis of a ytfG gene product from Escherichia coli

AU - Kim, In Kwon

AU - Yim, Hyung Soon

AU - Kim, Dong Won

AU - Kim, Young Min

AU - Lee, Heung Soo

AU - Cha, Sun Shin

AU - Kang, Sa Ouk

PY - 2004/2

Y1 - 2004/2

N2 - The Escherichia coli ytfG gene product, with NAD(P)H:quinone oxidoreductase activity, was crystallized by the hanging-drop vapour-diffusion method at 296 K. A 1.78 Å data set has been collected using synchrotron radiation at Pohang Light Source, South Korea. The crystal belongs to the primitive trigonal system, with unit-cell parameters a = b = 81.7, c = 76.8 Å. Analysis of the packing density shows that the asymmetric unit probably contains one monomer, with a solvent content of 48.8%.

AB - The Escherichia coli ytfG gene product, with NAD(P)H:quinone oxidoreductase activity, was crystallized by the hanging-drop vapour-diffusion method at 296 K. A 1.78 Å data set has been collected using synchrotron radiation at Pohang Light Source, South Korea. The crystal belongs to the primitive trigonal system, with unit-cell parameters a = b = 81.7, c = 76.8 Å. Analysis of the packing density shows that the asymmetric unit probably contains one monomer, with a solvent content of 48.8%.

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JO - Acta Crystallographica Section D: Biological Crystallography

JF - Acta Crystallographica Section D: Biological Crystallography

IS - 2

ER -

Crystallization and preliminary X-ray crystallographic analysis of a ytfG gene product from Escherichia coli

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