Crystallization and preliminary X-ray crystallographic analysis of the pediocin immunity protein (PedB) from Pediococcus pentosaceus at 1.35 Å resolution

In Kwon Kim; Min Kyu Kim; Hyung Soon Yim; Sun Shin Cha; Sa Ouk Kang

doi:10.1016/j.bbapap.2005.06.004

Crystallization and preliminary X-ray crystallographic analysis of the pediocin immunity protein (PedB) from Pediococcus pentosaceus at 1.35 Å resolution

In Kwon Kim, Min Kyu Kim, Hyung Soon Yim, Sun Shin Cha, Sa Ouk Kang

Department of Chemistry & Nanoscience

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

PedB, a bacterial immunity protein conferring immunity to a newly identified pediocin (pediocin PP-1), was crystallized by the hanging-drop vapor diffusion method at 296 K. A 1.35 Å data set has been collected from a single crystal at 100 K using synchrotron-radiation source. The PedB crystals belong to the hexagonal space group P6₂ or P6₄, with unit cell parameters a = b = 62.2, c = 39.9 Å. Analysis of the packing density shows that the asymmetric unit probably contains one molecule with a solvent content of 33.8%.

Original language	English
Pages (from-to)	205-208
Number of pages	4
Journal	Biochimica et Biophysica Acta - Proteins and Proteomics
Volume	1751
Issue number	2
DOIs	https://doi.org/10.1016/j.bbapap.2005.06.004
State	Published - 10 Aug 2005

Bibliographical note

Funding Information:
Work performed at Seoul National University was supported by the 21C Frontier Microbial Genomics and Application Center Program, Ministry of Science and Technology (Grant MG02-0201-001-1-0-0), Republic of Korea and by research fellowship of the BK21 project and work performed at Pohang Accelerator Laboratory was supported by a grant from the Biogreen 21 Project, Rural Development Administration, Republic of Korea.

Keywords

Bacteriocin
Immunity protein
PedB
Pediocin

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10.1016/j.bbapap.2005.06.004

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@article{5a922b2e4cc343b4862866d79c23d0f8,

title = "Crystallization and preliminary X-ray crystallographic analysis of the pediocin immunity protein (PedB) from Pediococcus pentosaceus at 1.35 {\AA} resolution",

abstract = "PedB, a bacterial immunity protein conferring immunity to a newly identified pediocin (pediocin PP-1), was crystallized by the hanging-drop vapor diffusion method at 296 K. A 1.35 {\AA} data set has been collected from a single crystal at 100 K using synchrotron-radiation source. The PedB crystals belong to the hexagonal space group P62 or P64, with unit cell parameters a = b = 62.2, c = 39.9 {\AA}. Analysis of the packing density shows that the asymmetric unit probably contains one molecule with a solvent content of 33.8%.",

keywords = "Bacteriocin, Immunity protein, PedB, Pediocin",

author = "Kim, {In Kwon} and Kim, {Min Kyu} and Yim, {Hyung Soon} and Cha, {Sun Shin} and Kang, {Sa Ouk}",

note = "Funding Information: Work performed at Seoul National University was supported by the 21C Frontier Microbial Genomics and Application Center Program, Ministry of Science and Technology (Grant MG02-0201-001-1-0-0), Republic of Korea and by research fellowship of the BK21 project and work performed at Pohang Accelerator Laboratory was supported by a grant from the Biogreen 21 Project, Rural Development Administration, Republic of Korea.",

year = "2005",

month = aug,

day = "10",

doi = "10.1016/j.bbapap.2005.06.004",

language = "English",

volume = "1751",

pages = "205--208",

journal = "Biochimica et Biophysica Acta - Proteins and Proteomics",

issn = "1570-9639",

publisher = "Elsevier",

number = "2",

}

Crystallization and preliminary X-ray crystallographic analysis of the pediocin immunity protein (PedB) from Pediococcus pentosaceus at 1.35 Å resolution. / Kim, In Kwon; Kim, Min Kyu; Yim, Hyung Soon et al.
In: Biochimica et Biophysica Acta - Proteins and Proteomics, Vol. 1751, No. 2, 10.08.2005, p. 205-208.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Crystallization and preliminary X-ray crystallographic analysis of the pediocin immunity protein (PedB) from Pediococcus pentosaceus at 1.35 Å resolution

AU - Kim, In Kwon

AU - Kim, Min Kyu

AU - Yim, Hyung Soon

AU - Cha, Sun Shin

AU - Kang, Sa Ouk

N1 - Funding Information: Work performed at Seoul National University was supported by the 21C Frontier Microbial Genomics and Application Center Program, Ministry of Science and Technology (Grant MG02-0201-001-1-0-0), Republic of Korea and by research fellowship of the BK21 project and work performed at Pohang Accelerator Laboratory was supported by a grant from the Biogreen 21 Project, Rural Development Administration, Republic of Korea.

PY - 2005/8/10

Y1 - 2005/8/10

N2 - PedB, a bacterial immunity protein conferring immunity to a newly identified pediocin (pediocin PP-1), was crystallized by the hanging-drop vapor diffusion method at 296 K. A 1.35 Å data set has been collected from a single crystal at 100 K using synchrotron-radiation source. The PedB crystals belong to the hexagonal space group P62 or P64, with unit cell parameters a = b = 62.2, c = 39.9 Å. Analysis of the packing density shows that the asymmetric unit probably contains one molecule with a solvent content of 33.8%.

AB - PedB, a bacterial immunity protein conferring immunity to a newly identified pediocin (pediocin PP-1), was crystallized by the hanging-drop vapor diffusion method at 296 K. A 1.35 Å data set has been collected from a single crystal at 100 K using synchrotron-radiation source. The PedB crystals belong to the hexagonal space group P62 or P64, with unit cell parameters a = b = 62.2, c = 39.9 Å. Analysis of the packing density shows that the asymmetric unit probably contains one molecule with a solvent content of 33.8%.

KW - Bacteriocin

KW - Immunity protein

KW - PedB

KW - Pediocin

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AN - SCOPUS:23444452560

SN - 1570-9639

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JO - Biochimica et Biophysica Acta - Proteins and Proteomics

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IS - 2

ER -

Crystallization and preliminary X-ray crystallographic analysis of the pediocin immunity protein (PedB) from Pediococcus pentosaceus at 1.35 Å resolution

Abstract

Bibliographical note

Keywords

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