Crystallization and preliminary X-ray crystallographic analysis of the pediocin immunity protein (PedB) from Pediococcus pentosaceus at 1.35 Å resolution

In Kwon Kim, Min Kyu Kim, Hyung Soon Yim, Sun Shin Cha, Sa Ouk Kang

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

PedB, a bacterial immunity protein conferring immunity to a newly identified pediocin (pediocin PP-1), was crystallized by the hanging-drop vapor diffusion method at 296 K. A 1.35 Å data set has been collected from a single crystal at 100 K using synchrotron-radiation source. The PedB crystals belong to the hexagonal space group P62 or P64, with unit cell parameters a = b = 62.2, c = 39.9 Å. Analysis of the packing density shows that the asymmetric unit probably contains one molecule with a solvent content of 33.8%.

Original languageEnglish
Pages (from-to)205-208
Number of pages4
JournalBiochimica et Biophysica Acta - Proteins and Proteomics
Volume1751
Issue number2
DOIs
StatePublished - 10 Aug 2005

Keywords

  • Bacteriocin
  • Immunity protein
  • PedB
  • Pediocin

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