Crystallization and preliminary X-ray crystallographic analysis of the protease inhibitor ecotin

Dong Hae Shin; Kwang Yeon Hwang; Kyeong Kyu Kim; Hae Ryun Lee; Cheol Soon Lee; Chin Ha Chung; Se Won Suh

doi:10.1006/jmbi.1993.1112

Crystallization and preliminary X-ray crystallographic analysis of the protease inhibitor ecotin

Dong Hae Shin, Kwang Yeon Hwang, Kyeong Kyu Kim, Hae Ryun Lee, Cheol Soon Lee, Chin Ha Chung, Se Won Suh

Department of Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Ecotin, a novel serine protease inhibitor isolated from Escherichia coli, has been crystallized using polyethylene glycol 1500 as the precipitating agent. The crystals belong to the orthorhombic space group P2₁2₁2₁, with unit cell parameters of a = 39.22 Å, b = 84.86 Å, and c = 98.74 Å. The asymmetric unit contains one dimeric molecule of ecotin, with a crystal volume per protein mass (V_m) of 2.55 Å³/Da and a solvent content of 51.8% by volume. The crystals diffract to at least 2.2 Å using a conventional X-ray source, and X-ray data have been collected to 2.7 Å Bragg spacing from a native crystal.

Original language	English
Pages (from-to)	1157-1158
Number of pages	2
Journal	Journal of Molecular Biology
Volume	229
Issue number	4
DOIs	https://doi.org/10.1006/jmbi.1993.1112
State	Published - 20 Feb 1993

Keywords

Crystallization
Ecotin
Preliminary X-ray analysis
Serine protease inhibitor
X-ray crystallography

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10.1006/jmbi.1993.1112

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title = "Crystallization and preliminary X-ray crystallographic analysis of the protease inhibitor ecotin",

abstract = "Ecotin, a novel serine protease inhibitor isolated from Escherichia coli, has been crystallized using polyethylene glycol 1500 as the precipitating agent. The crystals belong to the orthorhombic space group P212121, with unit cell parameters of a = 39.22 {\AA}, b = 84.86 {\AA}, and c = 98.74 {\AA}. The asymmetric unit contains one dimeric molecule of ecotin, with a crystal volume per protein mass (Vm) of 2.55 {\AA}3/Da and a solvent content of 51.8% by volume. The crystals diffract to at least 2.2 {\AA} using a conventional X-ray source, and X-ray data have been collected to 2.7 {\AA} Bragg spacing from a native crystal.",

keywords = "Crystallization, Ecotin, Preliminary X-ray analysis, Serine protease inhibitor, X-ray crystallography",

author = "Shin, {Dong Hae} and Hwang, {Kwang Yeon} and Kim, {Kyeong Kyu} and Lee, {Hae Ryun} and Lee, {Cheol Soon} and Chung, {Chin Ha} and Suh, {Se Won}",

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language = "English",

volume = "229",

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journal = "Journal of Molecular Biology",

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T1 - Crystallization and preliminary X-ray crystallographic analysis of the protease inhibitor ecotin

AU - Shin, Dong Hae

AU - Hwang, Kwang Yeon

AU - Kim, Kyeong Kyu

AU - Lee, Hae Ryun

AU - Lee, Cheol Soon

AU - Chung, Chin Ha

AU - Suh, Se Won

PY - 1993/2/20

Y1 - 1993/2/20

N2 - Ecotin, a novel serine protease inhibitor isolated from Escherichia coli, has been crystallized using polyethylene glycol 1500 as the precipitating agent. The crystals belong to the orthorhombic space group P212121, with unit cell parameters of a = 39.22 Å, b = 84.86 Å, and c = 98.74 Å. The asymmetric unit contains one dimeric molecule of ecotin, with a crystal volume per protein mass (Vm) of 2.55 Å3/Da and a solvent content of 51.8% by volume. The crystals diffract to at least 2.2 Å using a conventional X-ray source, and X-ray data have been collected to 2.7 Å Bragg spacing from a native crystal.

AB - Ecotin, a novel serine protease inhibitor isolated from Escherichia coli, has been crystallized using polyethylene glycol 1500 as the precipitating agent. The crystals belong to the orthorhombic space group P212121, with unit cell parameters of a = 39.22 Å, b = 84.86 Å, and c = 98.74 Å. The asymmetric unit contains one dimeric molecule of ecotin, with a crystal volume per protein mass (Vm) of 2.55 Å3/Da and a solvent content of 51.8% by volume. The crystals diffract to at least 2.2 Å using a conventional X-ray source, and X-ray data have been collected to 2.7 Å Bragg spacing from a native crystal.

KW - Crystallization

KW - Ecotin

KW - Preliminary X-ray analysis

KW - Serine protease inhibitor

KW - X-ray crystallography

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U2 - 10.1006/jmbi.1993.1112

DO - 10.1006/jmbi.1993.1112

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EP - 1158

JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

IS - 4

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Crystallization and preliminary X-ray crystallographic analysis of the protease inhibitor ecotin

Abstract

Keywords

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