Crystal structure of the DUF16 domain of MPN010 from Mycoplasma pneumoniae

Dong Hae Shin, Jeong Sun Kim, Hisao Yokota, Rosalind Kim, Sung Hou Kim

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6 Scopus citations


We have determined the crystal structure of the DUF16 domain of unknown function encoded by the gene MPN010 of Mycoplasma pneumoniae at 1.8 Å resolution. The crystal structure revealed that this domain is composed of two separated homotrimeric coiled-coils. The shorter one consists of 11 highly conserved residues. The sequence comprises noncanonical heptad repeats that induce a right-handed coiled-coil structure. The longer one is composed of approximately nine heptad repeats. In this coiled-coil structure, there are three distinguishable regions that confer unique structural properties compared with other known homotrimeric coiled-coils. The first part, containing one stutter, is an unusual phenylalanine-rich region that is not found in any other coiled-coil structures. The second part is a highly conserved glutamine-rich region, frequently found in other trimeric coiled-coil structures. The last part is composed of prototype heptad repeats. The phylogenetic analysis of the DUF16 family together with a secondary structure prediction shows that the DUF16 family can be classified into five subclasses according to N-terminal sequences. Based on the structural comparison with other coiled-coil structures, a probable molecular function of the DUF16 family is discussed.

Original languageEnglish
Pages (from-to)921-928
Number of pages8
JournalProtein Science
Issue number4
StatePublished - 1 Apr 2006


  • Coiled-coil
  • DUF16
  • Hypothetical protein
  • MPN010
  • Stutter


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