Crystal structure of D-glycero-Β-D-manno-heptose-1-phosphate adenylyltransferase from Burkholderia pseudomallei

Jimin Park, Hyojin Kim, Suwon Kim, Daeun Lee, Mi Sun Kim, Dong Hae Shin

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


The crystal structure of HldC from B. pseudomallei (BpHldC), the fourth enzyme of the heptose biosynthesis pathway, has been determined. BpHldC converts ATP and d-glycero-β-d-manno-heptose-1-phosphate into ADP-d-glycero-β-d-manno-heptose and pyrophosphate. The crystal structure of BpHldC belongs to the nucleotidyltransferase α/β phosphodiesterase superfamily sharing a common Rossmann-like α/β fold with a conserved T/HXGH sequence motif. The invariant catalytic key residues of BpHldC indicate that the core catalytic mechanism of BpHldC may be similar to that of other closest homologues. Intriguingly, a reorientation of the C-terminal helix seems to guide open and close states of the active site for the catalytic reaction.

Original languageEnglish
Pages (from-to)124-131
Number of pages8
JournalProteins: Structure, Function and Bioinformatics
Issue number1
StatePublished - Jan 2018

Bibliographical note

Funding Information:
We are grateful to Dr. Sarinna Tumapa at Mahidol University and Dr. Sharon Peacock at University of Cambridge for their kindness in providing us with B. pseudomallei genomic DNA and the staff at Pohang Light Source for their assistance with synchrotron data collection. This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2015R1D1A1A01058942). J. Park was supported by Brain Korea 21 (BK21) Project.

Publisher Copyright:
© 2017 Wiley Periodicals, Inc.


  • HldC
  • heptose biosynthesis pathway
  • lipopolysaccharide
  • melioidosis
  • nucleotidyltransferase


Dive into the research topics of 'Crystal structure of D-glycero-Β-D-manno-heptose-1-phosphate adenylyltransferase from Burkholderia pseudomallei'. Together they form a unique fingerprint.

Cite this