Crosstalk between platelet-derived growth factor-induced Nox4 activation and MUC8 gene overexpression in human airway epithelial cells

Hyun Jik Kim; Chang Hoon Kim; Ji Hwan Ryu; Jung Hee Joo; Sang Nam Lee; Min Ji Kim; Jeung Gweon Lee; Yun Soo Bae; Joo Heon Yoon

doi:10.1016/j.freeradbiomed.2011.01.014

Crosstalk between platelet-derived growth factor-induced Nox4 activation and MUC8 gene overexpression in human airway epithelial cells

Hyun Jik Kim, Chang Hoon Kim, Ji Hwan Ryu, Jung Hee Joo, Sang Nam Lee, Min Ji Kim, Jeung Gweon Lee, Yun Soo Bae, Joo Heon Yoon

Department of Life Science

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

Reactive oxygen species (ROS) contribute to chronic airway inflammation, and NADPH oxidase (Nox) is an important source of ROS. However, little is known of the role that ROS play in chronic upper respiratory tract inflammation. We investigated the mechanism of ROS generation and its association with mucin gene overexpression in the nasal epithelium. The level of platelet-derived growth factor (PDGF) expression was increased in sinusitis mucosa, and high-level PDGF expression induced intracellular ROS, followed by MUC8 gene overexpression in normal human nasal epithelial cells. Knockdown of Nox4 expression with Nox4 siRNA decreased PDGF-induced intracellular ROS and MUC8 expression. Infection with an adenovirus containing Nox4 cDNA resulted in Nox4 overexpression and increased intracellular levels of ROS and MUC8 expression. PDGF and Nox4 overexpression are essential components of intracellular ROS generation and may contribute to chronic inflammation in the nasal epithelium through induction of MUC8 overexpression.

Original language	English
Pages (from-to)	1039-1052
Number of pages	14
Journal	Free Radical Biology and Medicine
Volume	50
Issue number	9
DOIs	https://doi.org/10.1016/j.freeradbiomed.2011.01.014
State	Published - 1 May 2011

Bibliographical note

Funding Information:
This work was supported by a Korea Science and Engineering Foundation SRC grant funded by the Korean government (MOST) (R11-2007-040-02001-0) and a Korean Research Foundation grant funded by the Korean government (MOEHRD, Basic Research Promotion Fund) (KRF-2008-331-E00222).

Keywords

Free radicals
MUC8
Nox4
Platelet-derived growth factor
Reactive oxygen species

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10.1016/j.freeradbiomed.2011.01.014

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title = "Crosstalk between platelet-derived growth factor-induced Nox4 activation and MUC8 gene overexpression in human airway epithelial cells",

abstract = "Reactive oxygen species (ROS) contribute to chronic airway inflammation, and NADPH oxidase (Nox) is an important source of ROS. However, little is known of the role that ROS play in chronic upper respiratory tract inflammation. We investigated the mechanism of ROS generation and its association with mucin gene overexpression in the nasal epithelium. The level of platelet-derived growth factor (PDGF) expression was increased in sinusitis mucosa, and high-level PDGF expression induced intracellular ROS, followed by MUC8 gene overexpression in normal human nasal epithelial cells. Knockdown of Nox4 expression with Nox4 siRNA decreased PDGF-induced intracellular ROS and MUC8 expression. Infection with an adenovirus containing Nox4 cDNA resulted in Nox4 overexpression and increased intracellular levels of ROS and MUC8 expression. PDGF and Nox4 overexpression are essential components of intracellular ROS generation and may contribute to chronic inflammation in the nasal epithelium through induction of MUC8 overexpression.",

keywords = "Free radicals, MUC8, Nox4, Platelet-derived growth factor, Reactive oxygen species",

author = "Kim, {Hyun Jik} and Kim, {Chang Hoon} and Ryu, {Ji Hwan} and Joo, {Jung Hee} and Lee, {Sang Nam} and Kim, {Min Ji} and Lee, {Jeung Gweon} and Bae, {Yun Soo} and Yoon, {Joo Heon}",

note = "Funding Information: This work was supported by a Korea Science and Engineering Foundation SRC grant funded by the Korean government (MOST) (R11-2007-040-02001-0) and a Korean Research Foundation grant funded by the Korean government (MOEHRD, Basic Research Promotion Fund) (KRF-2008-331-E00222).",

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AU - Kim, Hyun Jik

AU - Kim, Chang Hoon

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AU - Joo, Jung Hee

AU - Lee, Sang Nam

AU - Kim, Min Ji

AU - Lee, Jeung Gweon

AU - Bae, Yun Soo

AU - Yoon, Joo Heon

N1 - Funding Information: This work was supported by a Korea Science and Engineering Foundation SRC grant funded by the Korean government (MOST) (R11-2007-040-02001-0) and a Korean Research Foundation grant funded by the Korean government (MOEHRD, Basic Research Promotion Fund) (KRF-2008-331-E00222).

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Y1 - 2011/5/1

N2 - Reactive oxygen species (ROS) contribute to chronic airway inflammation, and NADPH oxidase (Nox) is an important source of ROS. However, little is known of the role that ROS play in chronic upper respiratory tract inflammation. We investigated the mechanism of ROS generation and its association with mucin gene overexpression in the nasal epithelium. The level of platelet-derived growth factor (PDGF) expression was increased in sinusitis mucosa, and high-level PDGF expression induced intracellular ROS, followed by MUC8 gene overexpression in normal human nasal epithelial cells. Knockdown of Nox4 expression with Nox4 siRNA decreased PDGF-induced intracellular ROS and MUC8 expression. Infection with an adenovirus containing Nox4 cDNA resulted in Nox4 overexpression and increased intracellular levels of ROS and MUC8 expression. PDGF and Nox4 overexpression are essential components of intracellular ROS generation and may contribute to chronic inflammation in the nasal epithelium through induction of MUC8 overexpression.

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KW - Free radicals

KW - MUC8

KW - Nox4

KW - Platelet-derived growth factor

KW - Reactive oxygen species

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Crosstalk between platelet-derived growth factor-induced Nox4 activation and MUC8 gene overexpression in human airway epithelial cells

Abstract

Bibliographical note

Keywords

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