Cross-protection against Vibrio cholerae infection by monoclonal antibodies against Vibrio vulnificus RtxA1/MARTXVv

Tae Hee Lee; Sun Shin Cha; Chang Seop Lee; Joon Haeng Rhee; Hye Ryun Woo; Kyung Min Chung

doi:10.1111/1348-0421.12449

Cross-protection against Vibrio cholerae infection by monoclonal antibodies against Vibrio vulnificus RtxA1/MARTX_Vv

Tae Hee Lee, Sun Shin Cha, Chang Seop Lee, Joon Haeng Rhee, Hye Ryun Woo, Kyung Min Chung

Department of Chemistry & Nanoscience

Research output: Contribution to journal › Article › peer-review

Abstract

Gram-negative Vibrio species secrete multifunctional autoprocessing repeats-in-toxin (MARTX) toxins associated with bacterial pathogenesis. Here, the cross-reactivity and cross-protectivity of mAbs against V. vulnificus RtxA1/MARTX_Vv was evaluated. Passive administration of any of these mAbs (21RA, 24RA, 46RA, 47RA and 50RA) provided strong protection against lethal V. cholerae infection. Interestingly, 24RA and 46RA, which map to the cysteine protease domain of V. cholerae MARTX_Vc, inhibited CPD autocleavage in vitro; this process is involved in V. cholerae pathogenesis. These results generate new insight into the development of broadly protective mAbs and/or vaccines against Vibrio species with MARTX toxins.

Original language	English
Pages (from-to)	793-800
Number of pages	8
Journal	Microbiology and Immunology
Volume	60
Issue number	11
DOIs	https://doi.org/10.1111/1348-0421.12449
State	Published - 1 Nov 2016

Bibliographical note

Funding Information:
We thank Mi Hyun Kim and Hyo Seon Park for technical suggestions and experimental assistance. This work was supported by the Basic Science Research Program (NRF-2015R1D1A1A01056671) of the National Research Foundation of Korea, which is funded by the Ministry of Education.

Publisher Copyright:
© 2016 The Societies and John Wiley & Sons Australia, Ltd

Keywords

MARTX toxin
Vibrio species
cross-protection
monoclonal antibody

Access to Document

10.1111/1348-0421.12449

Cite this

@article{4cdf6d9af1724fa0bd5ef532c7f68d3e,

title = "Cross-protection against Vibrio cholerae infection by monoclonal antibodies against Vibrio vulnificus RtxA1/MARTXVv ",

abstract = "Gram-negative Vibrio species secrete multifunctional autoprocessing repeats-in-toxin (MARTX) toxins associated with bacterial pathogenesis. Here, the cross-reactivity and cross-protectivity of mAbs against V. vulnificus RtxA1/MARTXVv was evaluated. Passive administration of any of these mAbs (21RA, 24RA, 46RA, 47RA and 50RA) provided strong protection against lethal V. cholerae infection. Interestingly, 24RA and 46RA, which map to the cysteine protease domain of V. cholerae MARTXVc, inhibited CPD autocleavage in vitro; this process is involved in V. cholerae pathogenesis. These results generate new insight into the development of broadly protective mAbs and/or vaccines against Vibrio species with MARTX toxins.",

keywords = "MARTX toxin, Vibrio species, cross-protection, monoclonal antibody",

author = "Lee, {Tae Hee} and Cha, {Sun Shin} and Lee, {Chang Seop} and Rhee, {Joon Haeng} and Woo, {Hye Ryun} and Chung, {Kyung Min}",

note = "Funding Information: We thank Mi Hyun Kim and Hyo Seon Park for technical suggestions and experimental assistance. This work was supported by the Basic Science Research Program (NRF-2015R1D1A1A01056671) of the National Research Foundation of Korea, which is funded by the Ministry of Education. Publisher Copyright: {\textcopyright} 2016 The Societies and John Wiley & Sons Australia, Ltd",

year = "2016",

month = nov,

day = "1",

doi = "10.1111/1348-0421.12449",

language = "English",

volume = "60",

pages = "793--800",

journal = "Microbiology and Immunology",

issn = "0385-5600",

publisher = "Wiley-Blackwell",

number = "11",

}

TY - JOUR

T1 - Cross-protection against Vibrio cholerae infection by monoclonal antibodies against Vibrio vulnificus RtxA1/MARTXVv

AU - Lee, Tae Hee

AU - Cha, Sun Shin

AU - Lee, Chang Seop

AU - Rhee, Joon Haeng

AU - Woo, Hye Ryun

AU - Chung, Kyung Min

N1 - Funding Information: We thank Mi Hyun Kim and Hyo Seon Park for technical suggestions and experimental assistance. This work was supported by the Basic Science Research Program (NRF-2015R1D1A1A01056671) of the National Research Foundation of Korea, which is funded by the Ministry of Education. Publisher Copyright: © 2016 The Societies and John Wiley & Sons Australia, Ltd

PY - 2016/11/1

Y1 - 2016/11/1

N2 - Gram-negative Vibrio species secrete multifunctional autoprocessing repeats-in-toxin (MARTX) toxins associated with bacterial pathogenesis. Here, the cross-reactivity and cross-protectivity of mAbs against V. vulnificus RtxA1/MARTXVv was evaluated. Passive administration of any of these mAbs (21RA, 24RA, 46RA, 47RA and 50RA) provided strong protection against lethal V. cholerae infection. Interestingly, 24RA and 46RA, which map to the cysteine protease domain of V. cholerae MARTXVc, inhibited CPD autocleavage in vitro; this process is involved in V. cholerae pathogenesis. These results generate new insight into the development of broadly protective mAbs and/or vaccines against Vibrio species with MARTX toxins.

AB - Gram-negative Vibrio species secrete multifunctional autoprocessing repeats-in-toxin (MARTX) toxins associated with bacterial pathogenesis. Here, the cross-reactivity and cross-protectivity of mAbs against V. vulnificus RtxA1/MARTXVv was evaluated. Passive administration of any of these mAbs (21RA, 24RA, 46RA, 47RA and 50RA) provided strong protection against lethal V. cholerae infection. Interestingly, 24RA and 46RA, which map to the cysteine protease domain of V. cholerae MARTXVc, inhibited CPD autocleavage in vitro; this process is involved in V. cholerae pathogenesis. These results generate new insight into the development of broadly protective mAbs and/or vaccines against Vibrio species with MARTX toxins.

KW - MARTX toxin

KW - Vibrio species

KW - cross-protection

KW - monoclonal antibody

UR - http://www.scopus.com/inward/record.url?scp=85002044947&partnerID=8YFLogxK

U2 - 10.1111/1348-0421.12449

DO - 10.1111/1348-0421.12449

M3 - Article

C2 - 27921342

AN - SCOPUS:85002044947

SN - 0385-5600

VL - 60

SP - 793

EP - 800

JO - Microbiology and Immunology

JF - Microbiology and Immunology

IS - 11

ER -