Creatine Kinase B Is a Target Molecule of Reactive Oxygen Species in Cervical Cancer

Hyun Choi, Chang Soo Park, Byoung Gie Kim, Jae Won Cho, Jong Bae Park, Yun Soo Bae, Duk Soo Bae

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


Recently, a procedure for detecting ROS-sensitive proteins that contain active cysteine residues was developed. The method is based on the fact that biotin-conjugated iodoacetamide (BIAM) and ROS competitively and selectively react with the active cysteine residues in ROS-sensitive proteins. To investigate the role of ROS in cervical cancer, BIAM labeling on cytosolic proteins in normal and cancer tissues was performed, respectively. The BIAM labeling proteins are separated by 2-dimensional electrophoresis, and then identified by MALDI-TOF mass analysis. ROS-sensitive protein is identified as creatine kinase B containing cysteine residue in active center. Activity of creatine kinase B in normal tissue is higher than that of oxidized form in cervical cancer tissues. The result suggests that ROS play an important role in metabolic regulation in cervical cancer cells. However, molecular mechanisms that ROS and creatine kinase B are integrated into a physiological signal leading to the cellular transformation remain to be elucidated.

Original languageEnglish
Pages (from-to)412-417
Number of pages6
JournalMolecules and Cells
Issue number3
StatePublished - 31 Dec 2001


  • 2-Dimensional Gel Electrophoresis (2DE)
  • Biotinylation
  • Cervical Cancer
  • Creatine Kinase (CK)
  • Hydrogen Peroxide
  • Reactive Oxygen Epecies (ROS)


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