Cooperation and Functional Diversification of Two Closely Related Galactolipase Genes for Jasmonate Biosynthesis

Youbong Hyun; Sungwook Choi; Hyun Ju Hwang; Jihyeon Yu; Sang Jip Nam; Jaeyoung Ko; Ju Young Park; Young Sam Seo; Eun Yu Kim; Stephen Beungtae Ryu; Woo Taek Kim; Yong Hwan Lee; Heonjoong Kang; Ilha Lee

doi:10.1016/j.devcel.2007.11.010

Cooperation and Functional Diversification of Two Closely Related Galactolipase Genes for Jasmonate Biosynthesis

Youbong Hyun, Sungwook Choi, Hyun Ju Hwang, Jihyeon Yu, Sang Jip Nam, Jaeyoung Ko, Ju Young Park, Young Sam Seo, Eun Yu Kim, Stephen Beungtae Ryu, Woo Taek Kim, Yong Hwan Lee, Heonjoong Kang, Ilha Lee

Department of Chemistry & Nanoscience

Research output: Contribution to journal › Article › peer-review

144 Scopus citations

Abstract

Jasmonic acid (JA) plays pivotal roles in diverse plant biological processes, including wound response. Chloroplast lipid hydrolysis is a critical step for JA biosynthesis, but the mechanism of this process remains elusive. We report here that DONGLE (DGL), a homolog of DEFECTIVE IN ANTHER DEHISCENCE1 (DAD1), encodes a chloroplast-targeted lipase with strong galactolipase and weak phospholipase A₁ activity. DGL is expressed in the leaves and has a specific role in maintaining basal JA content under normal conditions, and this expression regulates vegetative growth and is required for a rapid JA burst after wounding. During wounding, DGL and DAD1 have partially redundant functions for JA production, but they show different induction kinetics, indicating temporally separated roles: DGL plays a role in the early phase of JA production, and DAD1 plays a role in the late phase of JA production. Whereas DGL and DAD1 are necessary and sufficient for JA production, phospholipase D appears to modulate wound response by stimulating DGL and DAD1 expression.

Original language	English
Pages (from-to)	183-192
Number of pages	10
Journal	Developmental Cell
Volume	14
Issue number	2
DOIs	https://doi.org/10.1016/j.devcel.2007.11.010
State	Published - 12 Feb 2008

Bibliographical note

Funding Information:
We thank X. Wang and J.G. Turner for kindly providing the pldα1 and cev1-1 seeds, R. Amasino for critical reading of the manuscript, and anonymous reviewers for critical comments. This work was supported partially by the Biogreen 21 Program (20050401-034-706-141-01-00); the Korea Ministry of Science and Technology under the National Research Laboratory Program (2006-01952); the Global Research Laboratory Program (2006-03870); and a grant from the Korea Science and Engineering Foundation through the Plant Metabolism Research Center, Kyung Hee University to I.L. S.-J.N. and H.K. were supported by MarineBio 21, the Ministry of Maritime Affairs & Fisheries, and Y.H., S.C., and J.Y. were supported by the Brain Korea 21 program.

Keywords

SIGNALING

Access to Document

10.1016/j.devcel.2007.11.010

Cite this

@article{67a2e2dd1f7549148c4d9f610e08e2ac,

title = "Cooperation and Functional Diversification of Two Closely Related Galactolipase Genes for Jasmonate Biosynthesis",

abstract = "Jasmonic acid (JA) plays pivotal roles in diverse plant biological processes, including wound response. Chloroplast lipid hydrolysis is a critical step for JA biosynthesis, but the mechanism of this process remains elusive. We report here that DONGLE (DGL), a homolog of DEFECTIVE IN ANTHER DEHISCENCE1 (DAD1), encodes a chloroplast-targeted lipase with strong galactolipase and weak phospholipase A1 activity. DGL is expressed in the leaves and has a specific role in maintaining basal JA content under normal conditions, and this expression regulates vegetative growth and is required for a rapid JA burst after wounding. During wounding, DGL and DAD1 have partially redundant functions for JA production, but they show different induction kinetics, indicating temporally separated roles: DGL plays a role in the early phase of JA production, and DAD1 plays a role in the late phase of JA production. Whereas DGL and DAD1 are necessary and sufficient for JA production, phospholipase D appears to modulate wound response by stimulating DGL and DAD1 expression.",

keywords = "SIGNALING",

author = "Youbong Hyun and Sungwook Choi and Hwang, {Hyun Ju} and Jihyeon Yu and Nam, {Sang Jip} and Jaeyoung Ko and Park, {Ju Young} and Seo, {Young Sam} and Kim, {Eun Yu} and Ryu, {Stephen Beungtae} and Kim, {Woo Taek} and Lee, {Yong Hwan} and Heonjoong Kang and Ilha Lee",

note = "Funding Information: We thank X. Wang and J.G. Turner for kindly providing the pldα1 and cev1-1 seeds, R. Amasino for critical reading of the manuscript, and anonymous reviewers for critical comments. This work was supported partially by the Biogreen 21 Program (20050401-034-706-141-01-00); the Korea Ministry of Science and Technology under the National Research Laboratory Program (2006-01952); the Global Research Laboratory Program (2006-03870); and a grant from the Korea Science and Engineering Foundation through the Plant Metabolism Research Center, Kyung Hee University to I.L. S.-J.N. and H.K. were supported by MarineBio 21, the Ministry of Maritime Affairs & Fisheries, and Y.H., S.C., and J.Y. were supported by the Brain Korea 21 program. ",

year = "2008",

month = feb,

day = "12",

doi = "10.1016/j.devcel.2007.11.010",

language = "English",

volume = "14",

pages = "183--192",

journal = "Developmental Cell",

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T1 - Cooperation and Functional Diversification of Two Closely Related Galactolipase Genes for Jasmonate Biosynthesis

AU - Hyun, Youbong

AU - Choi, Sungwook

AU - Hwang, Hyun Ju

AU - Yu, Jihyeon

AU - Nam, Sang Jip

AU - Ko, Jaeyoung

AU - Park, Ju Young

AU - Seo, Young Sam

AU - Kim, Eun Yu

AU - Ryu, Stephen Beungtae

AU - Kim, Woo Taek

AU - Lee, Yong Hwan

AU - Kang, Heonjoong

AU - Lee, Ilha

N1 - Funding Information: We thank X. Wang and J.G. Turner for kindly providing the pldα1 and cev1-1 seeds, R. Amasino for critical reading of the manuscript, and anonymous reviewers for critical comments. This work was supported partially by the Biogreen 21 Program (20050401-034-706-141-01-00); the Korea Ministry of Science and Technology under the National Research Laboratory Program (2006-01952); the Global Research Laboratory Program (2006-03870); and a grant from the Korea Science and Engineering Foundation through the Plant Metabolism Research Center, Kyung Hee University to I.L. S.-J.N. and H.K. were supported by MarineBio 21, the Ministry of Maritime Affairs & Fisheries, and Y.H., S.C., and J.Y. were supported by the Brain Korea 21 program.

PY - 2008/2/12

Y1 - 2008/2/12

N2 - Jasmonic acid (JA) plays pivotal roles in diverse plant biological processes, including wound response. Chloroplast lipid hydrolysis is a critical step for JA biosynthesis, but the mechanism of this process remains elusive. We report here that DONGLE (DGL), a homolog of DEFECTIVE IN ANTHER DEHISCENCE1 (DAD1), encodes a chloroplast-targeted lipase with strong galactolipase and weak phospholipase A1 activity. DGL is expressed in the leaves and has a specific role in maintaining basal JA content under normal conditions, and this expression regulates vegetative growth and is required for a rapid JA burst after wounding. During wounding, DGL and DAD1 have partially redundant functions for JA production, but they show different induction kinetics, indicating temporally separated roles: DGL plays a role in the early phase of JA production, and DAD1 plays a role in the late phase of JA production. Whereas DGL and DAD1 are necessary and sufficient for JA production, phospholipase D appears to modulate wound response by stimulating DGL and DAD1 expression.

AB - Jasmonic acid (JA) plays pivotal roles in diverse plant biological processes, including wound response. Chloroplast lipid hydrolysis is a critical step for JA biosynthesis, but the mechanism of this process remains elusive. We report here that DONGLE (DGL), a homolog of DEFECTIVE IN ANTHER DEHISCENCE1 (DAD1), encodes a chloroplast-targeted lipase with strong galactolipase and weak phospholipase A1 activity. DGL is expressed in the leaves and has a specific role in maintaining basal JA content under normal conditions, and this expression regulates vegetative growth and is required for a rapid JA burst after wounding. During wounding, DGL and DAD1 have partially redundant functions for JA production, but they show different induction kinetics, indicating temporally separated roles: DGL plays a role in the early phase of JA production, and DAD1 plays a role in the late phase of JA production. Whereas DGL and DAD1 are necessary and sufficient for JA production, phospholipase D appears to modulate wound response by stimulating DGL and DAD1 expression.

KW - SIGNALING

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U2 - 10.1016/j.devcel.2007.11.010

DO - 10.1016/j.devcel.2007.11.010

M3 - Article

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SN - 1534-5807

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