Construction of various bacteriophage λ mutants for stable and efficient production of recombinant protein in Escherichia coli

Jeong Seok Oh; Shin Sik Choi; Jong Kee Yeo; Tai Hyun Park

doi:10.1016/j.procbio.2006.09.014

Construction of various bacteriophage λ mutants for stable and efficient production of recombinant protein in Escherichia coli

Jeong Seok Oh, Shin Sik Choi, Jong Kee Yeo, Tai Hyun Park

Department of Nutritional Science & Food Management

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Three λ mutants were constructed based on the Q^- mutant in order to enhance their productivity and stability in an Escherichia coli/bacteriophage λ system. The newly constructed bacteriophage mutants named λSNU1, λSNU2, and λSNU3 were Q^-S^-, Q^-W^-E^-, and Q^-S^-W^-E^- mutants, respectively. Compared to all of the mutants, λSNU1 turned out to be the best with regards to higher protein expression and better genetic stability. Mechanisms by which these attributes are achieved have been discussed. The high productivity of P90c/λSNU1 for the recombinant protein was due to the high copy number of λ DNA and high translational efficiency. This mutant phage λSNU1 can be used to provide a high level of stability and productivity of the cloned gene particularly for long-term continuous operation.

Original language	English
Pages (from-to)	486-490
Number of pages	5
Journal	Process Biochemistry
Volume	42
Issue number	3
DOIs	https://doi.org/10.1016/j.procbio.2006.09.014
State	Published - Mar 2007

Bibliographical note

Funding Information:
This work was supported by the Korea Science & Engineering Foundation through the Nano-Bioelectronics and Systems Research Center, Seoul National University, Seoul, Korea.

Keywords

Bacteriophage λ
Cloned gene expression
E. coli
Late gene mutant
Plasmid stability
Q Mutant

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10.1016/j.procbio.2006.09.014

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title = "Construction of various bacteriophage λ mutants for stable and efficient production of recombinant protein in Escherichia coli",

abstract = "Three λ mutants were constructed based on the Q- mutant in order to enhance their productivity and stability in an Escherichia coli/bacteriophage λ system. The newly constructed bacteriophage mutants named λSNU1, λSNU2, and λSNU3 were Q-S-, Q-W-E-, and Q-S-W-E- mutants, respectively. Compared to all of the mutants, λSNU1 turned out to be the best with regards to higher protein expression and better genetic stability. Mechanisms by which these attributes are achieved have been discussed. The high productivity of P90c/λSNU1 for the recombinant protein was due to the high copy number of λ DNA and high translational efficiency. This mutant phage λSNU1 can be used to provide a high level of stability and productivity of the cloned gene particularly for long-term continuous operation.",

keywords = "Bacteriophage λ, Cloned gene expression, E. coli, Late gene mutant, Plasmid stability, Q Mutant",

author = "Oh, {Jeong Seok} and Choi, {Shin Sik} and Yeo, {Jong Kee} and Park, {Tai Hyun}",

note = "Funding Information: This work was supported by the Korea Science & Engineering Foundation through the Nano-Bioelectronics and Systems Research Center, Seoul National University, Seoul, Korea.",

year = "2007",

month = mar,

doi = "10.1016/j.procbio.2006.09.014",

language = "English",

volume = "42",

pages = "486--490",

journal = "Process Biochemistry",

issn = "1359-5113",

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T1 - Construction of various bacteriophage λ mutants for stable and efficient production of recombinant protein in Escherichia coli

AU - Oh, Jeong Seok

AU - Choi, Shin Sik

AU - Yeo, Jong Kee

AU - Park, Tai Hyun

N1 - Funding Information: This work was supported by the Korea Science & Engineering Foundation through the Nano-Bioelectronics and Systems Research Center, Seoul National University, Seoul, Korea.

PY - 2007/3

Y1 - 2007/3

N2 - Three λ mutants were constructed based on the Q- mutant in order to enhance their productivity and stability in an Escherichia coli/bacteriophage λ system. The newly constructed bacteriophage mutants named λSNU1, λSNU2, and λSNU3 were Q-S-, Q-W-E-, and Q-S-W-E- mutants, respectively. Compared to all of the mutants, λSNU1 turned out to be the best with regards to higher protein expression and better genetic stability. Mechanisms by which these attributes are achieved have been discussed. The high productivity of P90c/λSNU1 for the recombinant protein was due to the high copy number of λ DNA and high translational efficiency. This mutant phage λSNU1 can be used to provide a high level of stability and productivity of the cloned gene particularly for long-term continuous operation.

AB - Three λ mutants were constructed based on the Q- mutant in order to enhance their productivity and stability in an Escherichia coli/bacteriophage λ system. The newly constructed bacteriophage mutants named λSNU1, λSNU2, and λSNU3 were Q-S-, Q-W-E-, and Q-S-W-E- mutants, respectively. Compared to all of the mutants, λSNU1 turned out to be the best with regards to higher protein expression and better genetic stability. Mechanisms by which these attributes are achieved have been discussed. The high productivity of P90c/λSNU1 for the recombinant protein was due to the high copy number of λ DNA and high translational efficiency. This mutant phage λSNU1 can be used to provide a high level of stability and productivity of the cloned gene particularly for long-term continuous operation.

KW - Bacteriophage λ

KW - Cloned gene expression

KW - E. coli

KW - Late gene mutant

KW - Plasmid stability

KW - Q Mutant

UR - http://www.scopus.com/inward/record.url?scp=33847292413&partnerID=8YFLogxK

U2 - 10.1016/j.procbio.2006.09.014

DO - 10.1016/j.procbio.2006.09.014

M3 - Article

AN - SCOPUS:33847292413

SN - 1359-5113

VL - 42

SP - 486

EP - 490

JO - Process Biochemistry

JF - Process Biochemistry

IS - 3

ER -

Construction of various bacteriophage λ mutants for stable and efficient production of recombinant protein in Escherichia coli

Abstract

Bibliographical note

Keywords

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