Construction of Synthetic Promoter-Based Expression Cassettes for the Production of Cadaverine in Recombinant Corynebacterium glutamicum

Young Hoon Oh, Jae Woo Choi, Eun Young Kim, Bong Keun Song, Ki Jun Jeong, Kyungmoon Park, Il Kwon Kim, Han Min Woo, Seung Hwan Lee, Si Jae Park

Research output: Contribution to journalArticlepeer-review

40 Scopus citations

Abstract

Corynebacterium glutamicum is an important microorganism in the biochemical industry for the production of various platform chemicals. However, despite its importance, a limited number of studies have been conducted on how to constitute gene expression cassettes in engineered C. glutamicum to obtain desired amounts of the target products. Therefore, in this study, six expression cassettes for the expression of the second lysine decarboxylase of Escherichia coli, LdcC, were constructed using six synthetic promoters with different strengths and were examined in C. glutamicum for the production of cadaverine. Among six expression cassettes, the expression of the E. coli ldcC gene under the PH30 promoter supported the highest production of cadaverine in flask and fed-batch cultivations. A fed-batch fermentation of recombinant C. glutamicum expressing E. coli ldcC gene under the PH30 promoter resulted in the production of 40.91 g/L of cadaverine in 64 h. This report is expected to contribute toward developing engineered C. glutamicum strains to have desired features.

Original languageEnglish
Pages (from-to)2065-2075
Number of pages11
JournalApplied Biochemistry and Biotechnology
Volume176
Issue number7
DOIs
StatePublished - 17 Aug 2015

Keywords

  • C. glutamicum
  • Cadaverine
  • Fermentation
  • l-lysine
  • Synthetic promoters

Fingerprint

Dive into the research topics of 'Construction of Synthetic Promoter-Based Expression Cassettes for the Production of Cadaverine in Recombinant Corynebacterium glutamicum'. Together they form a unique fingerprint.

Cite this