Construction of heterologous gene expression cassettes for the development of recombinant Clostridium beijerinckii

Young Hoon Oh, Gyeong Tae Eom, Kyoung Hee Kang, Jeong Chan Joo, Young Ah Jang, Jae Woo Choi, Bong Keun Song, Seung Hwan Lee, Si Jae Park

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


Gene-expression cassettes for the construction of recombinant Clostridium beijerinckii were developed as potential tools for metabolic engineering of C. beijerinckii. Gene expression cassettes containing ColE1 origin and pAMB origin along with the erythromycin resistance gene were constructed, in which promoters from Escherichia coli, Lactococcus lactis, Ralstonia eutropha, C. acetobutylicum, and C. beijerinckii are examined as potential promoters in C. beijerinckii. Zymogram analysis of the cell extracts and comparison of lipase activities of the recombinant C. beijerinckii strains expressing Pseudomonas fluorescens tliA gene suggested that the tliA gene was functionally expressed by all the examined promoters with different expression level. Also, recombinant C. beijerinckii expressing C. beijerinckii secondary alcohol dehydrogenase by the constructed expression cassettes successfully produced 2-propanol from glucose. The best promoter for TliA expression was the R. eutropha phaP promoter while that for 2-propanol production was the putative C. beijerinckii pta promoter. Gene expression cassettes developed in this study may be useful tools for the construction of recombinant C. beijerinckii strains as host strains for the valuable chemicals and fuels from renewable resources.

Original languageEnglish
Pages (from-to)555-563
Number of pages9
JournalBioprocess and Biosystems Engineering
Issue number4
StatePublished - 1 Apr 2016

Bibliographical note

Funding Information:
This work was supported by the R&D Program of MOTIE/KEIT (10049674) and 2014 Research Fund of Myongji University.

Publisher Copyright:
© 2016 Springer-Verlag Berlin Heidelberg.


  • 2-Propanol
  • Clostridium beijerinckii
  • E. coli-Clostridia shuttle vector
  • Gene-expression cassettes
  • Lipase


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