Construction and evaluation of an automated light directed protein-detecting microarray synthesizer

N. Marthandan, S. Klyza, Shuwei Li, Yong Uk Kwon, T. Kodadek, H. R. Garner

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

We have designed, constructed, and evaluated an automated instrument that has produced high-density arrays with more than 30 000 peptide features within a 1.5 cm2 area of a glass slide surface. These arrays can be used for high throughput library screening for protein binding ligands, for potential drug candidate molecules, or for discovering biomarkers. The device consists of a novel fluidics system, a relay control electrical system, an optics system that implements Texas Instrumentspsila digital micromirror device (DMD), and a microwave source for accelerated synthesis of peptide arrays. The instrument implements two novel solid phase chemical synthesis strategies for producing peptide and peptoid arrays. Biotin-streptavidin and DNP anti-DNP (dinitrophenol) models of antibody small molecule interactions were used to demonstrate and evaluate the instrument's capability to produce high-density protein detecting arrays. Several screening assay and detection schemes were explored with various levels of efficiency and assays with sensitivity of 10 nM were also possible.

Original languageEnglish
Pages (from-to)20-27
Number of pages8
JournalIEEE Transactions on Nanobioscience
Volume7
Issue number1
DOIs
StatePublished - Mar 2008

Keywords

  • Antibody
  • Combinatorial chemistry
  • Digital micromirror device (DMD)
  • Fluorophores and dyes
  • Hybridization
  • Microarrays
  • Peptide
  • Peptoid
  • Photolithography
  • Protein
  • Screening assay
  • Streptavidin

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