Constituents of the leaves and twigs of Elaeagnus umbellata and their proliferative effects on human keratinocyte HaCaT cells

Sunil Babu Paudel, Jiyoung Park, Nam Hee Kim, Hyukjae Choi, Eun Kyoung Seo, Hyun Ae Woo, Joo Won Nam

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Bioassay-guided fractionation of an extract of leaves and twigs of Elaeagnus umbellata led to the isolation of a serotonin derivative, N-[2-(5-hydroxyl-1H-indol-3-yl)ethyl]-butanamide (1), along with six flavonoid glycosides, kaempferol-3-O-β-D-xylopyranosyl(1 → 2)-β-D-galactopyranoside-7-O-α-L-rhamnopyranoside (2), kaempferol-3-O-β-D-galactopyranoside-7-O-α-L-rhamnopyranoside (3), kaempferol-3-O-α-L-rhamnopyranosyl(1 → 6)-β-D-galactopyranoside-7-O-α-L-rhamnopyranoside (4), kaempferol-3-O-β-D-xylopyranosyl(1 → 2)-β-D-galactopyranoside (5), kaempferol-3-O-rutinoside (6), and kaempferol-3-O-β-D-glucopyranosyl(1 → 2)-β-D-galactopyranoside-7-O-α-L-rhamnopyranoside (7). Their structures were elucidated using 1D/2D nuclear magnetic resonance spectroscopy and mass spectrometry. Compounds 1–6 were evaluated for their proliferative effects on HaCaT keratinocytes; 1–5 promoted keratinocyte proliferation dose dependently. Compounds 3 and 4 showed potent activities. These results suggest that the leaves and twigs of E. umbellata have wound healing and skin cell regeneration potentials.

Original languageEnglish
Article number104374
JournalFitoterapia
Volume139
DOIs
StatePublished - Nov 2019

Keywords

  • Cell proliferation
  • Elaeagnaceae
  • Elaeagnus umbellata
  • Flavonoid glycoside
  • HaCaT keratinocyte
  • Serotonin derivative

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