Constituents of the leaves and twigs of Elaeagnus umbellata and their proliferative effects on human keratinocyte HaCaT cells

Sunil Babu Paudel, Jiyoung Park, Nam Hee Kim, Hyukjae Choi, Eun Kyoung Seo, Hyun Ae Woo, Joo Won Nam

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8 Scopus citations


Bioassay-guided fractionation of an extract of leaves and twigs of Elaeagnus umbellata led to the isolation of a serotonin derivative, N-[2-(5-hydroxyl-1H-indol-3-yl)ethyl]-butanamide (1), along with six flavonoid glycosides, kaempferol-3-O-β-D-xylopyranosyl(1 → 2)-β-D-galactopyranoside-7-O-α-L-rhamnopyranoside (2), kaempferol-3-O-β-D-galactopyranoside-7-O-α-L-rhamnopyranoside (3), kaempferol-3-O-α-L-rhamnopyranosyl(1 → 6)-β-D-galactopyranoside-7-O-α-L-rhamnopyranoside (4), kaempferol-3-O-β-D-xylopyranosyl(1 → 2)-β-D-galactopyranoside (5), kaempferol-3-O-rutinoside (6), and kaempferol-3-O-β-D-glucopyranosyl(1 → 2)-β-D-galactopyranoside-7-O-α-L-rhamnopyranoside (7). Their structures were elucidated using 1D/2D nuclear magnetic resonance spectroscopy and mass spectrometry. Compounds 1–6 were evaluated for their proliferative effects on HaCaT keratinocytes; 1–5 promoted keratinocyte proliferation dose dependently. Compounds 3 and 4 showed potent activities. These results suggest that the leaves and twigs of E. umbellata have wound healing and skin cell regeneration potentials.

Original languageEnglish
Article number104374
StatePublished - Nov 2019

Bibliographical note

Funding Information:
This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education [NRF-2016R1D1A3B03933996]. The work was also funded in part by a 2018 Yeungnam University Research Grant.

Publisher Copyright:
© 2019 Elsevier B.V.


  • Cell proliferation
  • Elaeagnaceae
  • Elaeagnus umbellata
  • Flavonoid glycoside
  • HaCaT keratinocyte
  • Serotonin derivative


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