Compositional changes in fecal microbiota associated with clinical phenotypes and prognosis in Korean patients with inflammatory bowel disease

Seung Yong Shin, Young Kim, Won Seok Kim, Jung Min Moon, Kang Moon Lee, Sung Ae Jung, Hyesook Park, Eun Young Huh, Byung Chang Kim, Soo Chan Lee, Chang Hwan Choi

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Background/Aims: The fecal microbiota of Korean patients with inflammatory bowel disease (IBD) was investigated with respect to disease phenotypes and taxonomic biomarkers for diagnosis and prognosis of IBD. Methods: Fecal samples from 70 ulcerative colitis (UC) patients, 39 Crohn’s disease (CD) patients, and 100 healthy control individuals (HC) were collected. The fecal samples were amplified via polymerase chain reaction and sequenced using Illumina MiSeq. The relationships between fecal bacteria and clinical phenotypes were analyzed using the EzBioCloud database and 16S microbiome pipeline. Results: The alpha-diversity of fecal bacteria was significantly lower in UC and CD (P<0.05) compared to that in HC. Bacterial community compositions in UC and CD were significantly different from that of HC according to Bray-Curtis dissimilarities, and there was also a difference between community composition in UC and CD (P=0.01). In UC, alpha-diversity was further decreased when the disease was more severe and the extent of disease was greater, and community composition significantly differed depending on the extent of the disease. We identified 9 biomarkers of severity and 6 biomarkers of the extent of UC. We also identified 5 biomarkers of active disease and 3 biomarkers of ileocolonic involvement in CD. Lachnospiraceae and Ruminococcus gnavus were biomarkers for better prognosis in CD. Conclusions: The fecal microbiota profiles of IBD patients were different from those of HC, and several bacterial taxa may be used as biomarkers to determine disease phenotypes and prognosis. These data may also help discover new therapeutic targets for IBD.

Original languageEnglish
Pages (from-to)148-160
Number of pages13
JournalIntestinal Research
Volume21
Issue number1
DOIs
StatePublished - 2023

Bibliographical note

Funding Information:
This work was funded by the National Research Foundation of Korea (NRF) (Grant/Award No. NRF-2020R1F1A1075489) and a grant of the Korean Association for the Study of Intestinal Diseases for 2009. Lee SC was supported by a CTSA/IIMS UT Health Pilot Grant and holds a Voelcker Fund Young Investigator Award from the Max and Minnie Tomerlin Voelcker Fund.

Publisher Copyright:
© Copyright 2023. Korean Association for the Study of Intestinal Diseases

Keywords

  • Biomarkers
  • Inflammatory bowel disease
  • Microbiota
  • Phenotype
  • Prognosis

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