@article{0d676050bb004adfbfb3d775d123e226,
title = "Complexation of drug and hapten-conjugated aptamer with universal hapten antibody for pancreatic cancer treatment",
abstract = "Owing to a lack of reliable markers and therapeutic targets, pancreatic ductal adenocarcinoma (PDAC) remains the most lethal malignant tumor despite numerous therapeutic advances. In this study, we utilized cell-SELEX to isolate a DNA aptamer recognizing the natural conformation of the target on the cell surface. PAp7T8, an aptamer optimized by size and chemical modification, exhibited specific targeting to pancreatic cancer cells and orthotopic xenograft pancreatic tumors. To confer therapeutic functions to the aptamer, we adopted a drug-conjugated oligobody (DOligobody) strategy. Monomethyl auristatin E was used as a cytotoxic drug, digoxigenin acted as a hapten, and the humanized anti-digoxigenin antibody served as a universal carrier of the aptamer. The resulting PAp7T8-DOligobody showed extended in vivo half-life and markedly inhibited tumor growth in an orthotopic pancreatic cancer xenograft model without causing significant toxicity. Therefore, PAp7T8-DOligobody represents a promising novel therapeutic delivery platform for PDAC.",
keywords = "Aptamer, Drug delivery, Oligobody, Pancreatic ductal adenocarcinoma, Targeted therapy",
author = "Choi, {Sun Il} and Lee, {Yu Sun} and Lee, {Yul Min} and Kim, {Hyun Jung} and Kim, {Won Jong} and Sungjin Jung and Im, {Ji Eun} and Lee, {Mi Rim} and Kim, {Joon Ki} and Jeon, {A. Ra} and Woo, {Sang Myung} and Oh, {Goo Taeg} and Kyun Heo and Kim, {Yun Hee} and Kim, {In Hoo}",
note = "Funding Information: This work was supported by the Research Core Center in National Cancer Center Korea . This study was supported by the Korea Technology and Information Promotion Agency for Small and Medium Enterprises [grant number: S2562351 ], National Cancer Center [grant number: NCC-1410270 & NCC-2310632 ], and the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning [grant number: NRF-2019R1A2C1010919 ]. The funders had no role in the design of the study; in the collection, analysis, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. The figures were created with BioRender.com . Publisher Copyright: {\textcopyright} 2023 The Authors",
year = "2023",
month = aug,
doi = "10.1016/j.jconrel.2023.03.048",
language = "English",
volume = "360",
pages = "940--952",
journal = "Journal of Controlled Release",
issn = "0168-3659",
publisher = "Elsevier",
}