Completing the canvas: advances and challenges for DNA-PAINT super-resolution imaging

Raman van Wee, Mike Filius, Chirlmin Joo

Research output: Contribution to journalReview articlepeer-review

12 Scopus citations

Abstract

Single-molecule localization microscopy (SMLM) is a potent tool to examine biological systems with unprecedented resolution, enabling the investigation of increasingly smaller structures. At the forefront of these developments is DNA-based point accumulation for imaging in nanoscale topography (DNA-PAINT), which exploits the stochastic and transient binding of fluorescently labeled DNA probes. In its early stages the implementation of DNA-PAINT was burdened by low-throughput, excessive acquisition time, and difficult integration with live-cell imaging. However, recent advances are addressing these challenges and expanding the range of applications of DNA-PAINT. We review the current state of the art of DNA-PAINT in light of these advances and contemplate what further developments remain indispensable to realize live-cell imaging.

Original languageEnglish
Pages (from-to)918-930
Number of pages13
JournalTrends in Biochemical Sciences
Volume46
Issue number11
DOIs
StatePublished - Nov 2021

Bibliographical note

Funding Information:
We thank Tao Ju Cui, Mingjie Dai, Kristin Grußmayer, Brian Analikwu, and Irene van den Bent for critical reading and feedback. C.J. was supported by the Vrije Programma (SMPS) of the Foundation for Fundamental Research on Matter and by a European Research Council ( ERC ) Consolidator grant ( 819299 ).

Publisher Copyright:
© 2021 Elsevier Ltd

Keywords

  • DNA-PAINT
  • acquisition speed
  • live-cell imaging
  • multiplexing
  • single-molecule localization microscopy
  • super-resolution microscopy

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