Abstract
Single-molecule localization microscopy (SMLM) is a potent tool to examine biological systems with unprecedented resolution, enabling the investigation of increasingly smaller structures. At the forefront of these developments is DNA-based point accumulation for imaging in nanoscale topography (DNA-PAINT), which exploits the stochastic and transient binding of fluorescently labeled DNA probes. In its early stages the implementation of DNA-PAINT was burdened by low-throughput, excessive acquisition time, and difficult integration with live-cell imaging. However, recent advances are addressing these challenges and expanding the range of applications of DNA-PAINT. We review the current state of the art of DNA-PAINT in light of these advances and contemplate what further developments remain indispensable to realize live-cell imaging.
Original language | English |
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Pages (from-to) | 918-930 |
Number of pages | 13 |
Journal | Trends in Biochemical Sciences |
Volume | 46 |
Issue number | 11 |
DOIs | |
State | Published - Nov 2021 |
Keywords
- DNA-PAINT
- acquisition speed
- live-cell imaging
- multiplexing
- single-molecule localization microscopy
- super-resolution microscopy