Background: Clostridium perfringens is an opportunistic human pathogen that causes necrotic enteritis, mild diarrhea, clostridial myonecrosis or gas gangrene, sepsis, etc. In this study, we aim to determine the pathogenesis of this bacterium at the genomic level. The genome of strain CBA7123 was sequenced, and a comparative genomic analysis between strain CBA7123 and four other related C. perfringens strains was performed. Results: The genome of strain CBA7123 consisted of one circular chromosome and one plasmid that were 3,088,370 and 46,640 bp long with 28.5 and 27.1 mol% G+C content, respectively. The genomic DNA was predicted to contain 2798 open reading frames (ORFs), 10 rRNA genes, and 94 tRNA genes. The genomic comparison analysis between the five strains revealed the distinctive virulence properties of strain CBA7123 by highlighting certain strain-specific genes. Conclusions: In this study, the C. perfringens CBA7123 genome was sequenced and compared with other C. perfringens genomes. Among the various genes sequenced, the detection of antimicrobial resistance genes and those encoding various virulence factors may extend the understanding of the pathogenesis of C. perfringens strains.
Bibliographical noteFunding Information:
This research was supported by the Korea Food Research Institute (E0170602‑ 01), the World Institute of Kimchi funded by the Ministry of Science, ICT and Future Planning, Republic of Korea (KE1702‑2), the Center for Analytical Research of Disaster Science of the Korea Basic Science Institute (C37703), and the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science, and Technology (2015R1D1A1A09061039).
© 2017 The Author(s).
- Antimicrobial resistant
- Clostridium perfringens
- Comparative genomic analysis
- Complete genome sequence
- Virulence factor