Comparison of microbial reduction effect of intense pulsed light according to growth stage and population density of Escherichia coli ATCC 25922 using a double Weibull model

Hee Jeong Hwang; Bo Won Park; Myong Soo Chung

doi:10.1016/j.foodres.2022.112353

Comparison of microbial reduction effect of intense pulsed light according to growth stage and population density of Escherichia coli ATCC 25922 using a double Weibull model

Hee Jeong Hwang, Bo Won Park, Myong Soo Chung

Department of Food Science & Biotechnology

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

This study evaluated how the efficacy of intense pulsed light (IPL) was influenced by biological factors such as the incubation time and the population of Escherichia coli. According to the 4D value, the microorganisms in the exponential phase were more susceptible to IPL (0.51 J/cm²), while those in the stationary phase were the most resistant (0.67 J/cm²). The microorganisms in the exponential phase could have more critical DNA damage. In addition, the degree of inactivation was affected by the microbial population. When the population was 10⁹ CFU/ml, a maximum 3.4-log reduction was observed after applying IPL at 12.5 J/cm². In contrast, a population with a density of 10¹⁰ CFU/ml showed maximally 0.13-log reduction when IPL was applied at 18.7 J/cm². This large difference might have been due to cell distribution and aggregation. The study is expected to contribute to the analytical confirmation of the microbial reduction mechanism through non-thermal technologies.

Original language	English
Article number	112353
Journal	Food Research International
Volume	164
DOIs	https://doi.org/10.1016/j.foodres.2022.112353
State	Published - Feb 2023

Bibliographical note

Funding Information:
This work was supported by the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry (IPET) through the High Value-Added Food Technology Development Program funded by the Ministry of Agriculture, Food and Rural Affairs (MAFRA) (121025-3) and the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2019R1A2C1085100). The paper was also supported by RP-Grant 2022 of Ewha Womans University.

Publisher Copyright:
© 2022 The Authors

Keywords

Cell aggregation
Exponential phase
Intense pulsed light
Microbial population

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10.1016/j.foodres.2022.112353

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title = "Comparison of microbial reduction effect of intense pulsed light according to growth stage and population density of Escherichia coli ATCC 25922 using a double Weibull model",

abstract = "This study evaluated how the efficacy of intense pulsed light (IPL) was influenced by biological factors such as the incubation time and the population of Escherichia coli. According to the 4D value, the microorganisms in the exponential phase were more susceptible to IPL (0.51 J/cm2), while those in the stationary phase were the most resistant (0.67 J/cm2). The microorganisms in the exponential phase could have more critical DNA damage. In addition, the degree of inactivation was affected by the microbial population. When the population was 109 CFU/ml, a maximum 3.4-log reduction was observed after applying IPL at 12.5 J/cm2. In contrast, a population with a density of 1010 CFU/ml showed maximally 0.13-log reduction when IPL was applied at 18.7 J/cm2. This large difference might have been due to cell distribution and aggregation. The study is expected to contribute to the analytical confirmation of the microbial reduction mechanism through non-thermal technologies.",

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author = "Hwang, {Hee Jeong} and Park, {Bo Won} and Chung, {Myong Soo}",

note = "Funding Information: This work was supported by the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry (IPET) through the High Value-Added Food Technology Development Program funded by the Ministry of Agriculture, Food and Rural Affairs (MAFRA) (121025-3) and the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2019R1A2C1085100). The paper was also supported by RP-Grant 2022 of Ewha Womans University. Publisher Copyright: {\textcopyright} 2022 The Authors",

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doi = "10.1016/j.foodres.2022.112353",

language = "English",

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AU - Hwang, Hee Jeong

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AU - Chung, Myong Soo

N1 - Funding Information: This work was supported by the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry (IPET) through the High Value-Added Food Technology Development Program funded by the Ministry of Agriculture, Food and Rural Affairs (MAFRA) (121025-3) and the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2019R1A2C1085100). The paper was also supported by RP-Grant 2022 of Ewha Womans University. Publisher Copyright: © 2022 The Authors

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Y1 - 2023/2

N2 - This study evaluated how the efficacy of intense pulsed light (IPL) was influenced by biological factors such as the incubation time and the population of Escherichia coli. According to the 4D value, the microorganisms in the exponential phase were more susceptible to IPL (0.51 J/cm2), while those in the stationary phase were the most resistant (0.67 J/cm2). The microorganisms in the exponential phase could have more critical DNA damage. In addition, the degree of inactivation was affected by the microbial population. When the population was 109 CFU/ml, a maximum 3.4-log reduction was observed after applying IPL at 12.5 J/cm2. In contrast, a population with a density of 1010 CFU/ml showed maximally 0.13-log reduction when IPL was applied at 18.7 J/cm2. This large difference might have been due to cell distribution and aggregation. The study is expected to contribute to the analytical confirmation of the microbial reduction mechanism through non-thermal technologies.

AB - This study evaluated how the efficacy of intense pulsed light (IPL) was influenced by biological factors such as the incubation time and the population of Escherichia coli. According to the 4D value, the microorganisms in the exponential phase were more susceptible to IPL (0.51 J/cm2), while those in the stationary phase were the most resistant (0.67 J/cm2). The microorganisms in the exponential phase could have more critical DNA damage. In addition, the degree of inactivation was affected by the microbial population. When the population was 109 CFU/ml, a maximum 3.4-log reduction was observed after applying IPL at 12.5 J/cm2. In contrast, a population with a density of 1010 CFU/ml showed maximally 0.13-log reduction when IPL was applied at 18.7 J/cm2. This large difference might have been due to cell distribution and aggregation. The study is expected to contribute to the analytical confirmation of the microbial reduction mechanism through non-thermal technologies.

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Comparison of microbial reduction effect of intense pulsed light according to growth stage and population density of Escherichia coli ATCC 25922 using a double Weibull model

Abstract

Bibliographical note

Keywords

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