Comparison of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry assay with conventional methods for detection of IMP-6, VIM-2, NDM-1, SIM-1, KPC-1, OXA-23, and OXA-51 carbapenemase-producing Acinetobacter spp., Pseudomonas aeruginosa, and Klebsiella pneumoniae

Wonmok Lee; Hae Sun Chung; Yangsoon Lee; Dongeun Yong; Seok Hoon Jeong; Kyungwon Lee; Yunsop Chong

doi:10.1016/j.diagmicrobio.2013.07.005

Comparison of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry assay with conventional methods for detection of IMP-6, VIM-2, NDM-1, SIM-1, KPC-1, OXA-23, and OXA-51 carbapenemase-producing Acinetobacter spp., Pseudomonas aeruginosa, and Klebsiella pneumoniae

Wonmok Lee
, Hae Sun Chung
, Yangsoon Lee
, Dongeun Yong
, Seok Hoon Jeong
, Kyungwon Lee
, Yunsop Chong

Department of Laboratory Medicine

Research output: Contribution to journal › Article › peer-review

39 Scopus citations

Abstract

Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry assay was able to detect carbapenemase producers, including SIM-1 or OXA-51, within 4 hours using 20 μL of 0.5 g/L ertapenem solution as a substrate. This assay is more rapid and accurate than the modified Hodge test and 3-dimensional extract bioassay. Hence, it can be used an alternative test to identify carbapenemase-mediated carbapenem resistance in Gram-negative bacteria.

Original language	English
Pages (from-to)	227-230
Number of pages	4
Journal	Diagnostic Microbiology and Infectious Disease
Volume	77
Issue number	3
DOIs	https://doi.org/10.1016/j.diagmicrobio.2013.07.005
State	Published - Nov 2013

Bibliographical note

Funding Information:
This study was supported by faculty research grant 6-2011-0100 from Yonsei University College of Medicine for 2012. We thank So-ri Jong and In Hae Kim for their excellent technical assistance.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Acinetobacter spp
Carbapenemase
MALDI-TOF MS
OXA
SIM-1

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Lee, W., Chung, H. S., Lee, Y., Yong, D., Jeong, S. H., Lee, K., & Chong, Y. (2013). Comparison of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry assay with conventional methods for detection of IMP-6, VIM-2, NDM-1, SIM-1, KPC-1, OXA-23, and OXA-51 carbapenemase-producing Acinetobacter spp., Pseudomonas aeruginosa, and Klebsiella pneumoniae. Diagnostic Microbiology and Infectious Disease, 77(3), 227-230. https://doi.org/10.1016/j.diagmicrobio.2013.07.005

Lee, Wonmok ; Chung, Hae Sun ; Lee, Yangsoon et al. / Comparison of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry assay with conventional methods for detection of IMP-6, VIM-2, NDM-1, SIM-1, KPC-1, OXA-23, and OXA-51 carbapenemase-producing Acinetobacter spp., Pseudomonas aeruginosa, and Klebsiella pneumoniae. In: Diagnostic Microbiology and Infectious Disease. 2013 ; Vol. 77, No. 3. pp. 227-230.

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title = "Comparison of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry assay with conventional methods for detection of IMP-6, VIM-2, NDM-1, SIM-1, KPC-1, OXA-23, and OXA-51 carbapenemase-producing Acinetobacter spp., Pseudomonas aeruginosa, and Klebsiella pneumoniae",

abstract = "Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry assay was able to detect carbapenemase producers, including SIM-1 or OXA-51, within 4 hours using 20 μL of 0.5 g/L ertapenem solution as a substrate. This assay is more rapid and accurate than the modified Hodge test and 3-dimensional extract bioassay. Hence, it can be used an alternative test to identify carbapenemase-mediated carbapenem resistance in Gram-negative bacteria.",

keywords = "Acinetobacter spp, Carbapenemase, MALDI-TOF MS, OXA, SIM-1",

author = "Wonmok Lee and Chung, \{Hae Sun\} and Yangsoon Lee and Dongeun Yong and Jeong, \{Seok Hoon\} and Kyungwon Lee and Yunsop Chong",

note = "Funding Information: This study was supported by faculty research grant 6-2011-0100 from Yonsei University College of Medicine for 2012. We thank So-ri Jong and In Hae Kim for their excellent technical assistance.",

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Lee, W, Chung, HS, Lee, Y, Yong, D, Jeong, SH, Lee, K & Chong, Y 2013, 'Comparison of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry assay with conventional methods for detection of IMP-6, VIM-2, NDM-1, SIM-1, KPC-1, OXA-23, and OXA-51 carbapenemase-producing Acinetobacter spp., Pseudomonas aeruginosa, and Klebsiella pneumoniae', Diagnostic Microbiology and Infectious Disease, vol. 77, no. 3, pp. 227-230. https://doi.org/10.1016/j.diagmicrobio.2013.07.005

Comparison of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry assay with conventional methods for detection of IMP-6, VIM-2, NDM-1, SIM-1, KPC-1, OXA-23, and OXA-51 carbapenemase-producing Acinetobacter spp., Pseudomonas aeruginosa, and Klebsiella pneumoniae. / Lee, Wonmok; Chung, Hae Sun; Lee, Yangsoon et al.
In: Diagnostic Microbiology and Infectious Disease, Vol. 77, No. 3, 11.2013, p. 227-230.

Research output: Contribution to journal › Article › peer-review

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AU - Lee, Yangsoon

AU - Yong, Dongeun

AU - Jeong, Seok Hoon

AU - Lee, Kyungwon

AU - Chong, Yunsop

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PY - 2013/11

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Lee W, Chung HS, Lee Y, Yong D, Jeong SH, Lee K et al. Comparison of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry assay with conventional methods for detection of IMP-6, VIM-2, NDM-1, SIM-1, KPC-1, OXA-23, and OXA-51 carbapenemase-producing Acinetobacter spp., Pseudomonas aeruginosa, and Klebsiella pneumoniae. Diagnostic Microbiology and Infectious Disease. 2013 Nov;77(3):227-230. doi: 10.1016/j.diagmicrobio.2013.07.005