Comparison of biochemical properties of the original and newly identified oleate hydratases from Stenotrophomonas maltophilia

Woo Ri Kang, Min Ju Seo, Kyung Chul Shin, Jin Byung Park, Deok Kun Oh

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Oleate hydratases (OhyAs) catalyze the conversion of unsaturated fatty acids to 10-hydroxy fatty acids, which are used as precursors of important industrial compounds, including lactones and ω-hydroxycarboxylic and α,ω-dicarboxylic acids. The genes encoding OhyA and a putative fatty acid hydratase in Stenotrophomonas maltophilia were identified by genomic analysis. The putative fatty acid hydratase was purified and identified as an oleate hydratase (OhyA2) based on its substrate specificity. The activity of OhyA2 as a holoenzyme was not affected by adding cofactors, whereas the activity of the original oleate hydratase (OhyA1) showed an increase. Thus, all characterized OhyAs were categorized as either OhyA1 or OhyA2 based on the activities of holoenzymes upon adding cofactors, which were determined by the type of the fourth conserved amino acid of flavin adenine dinucleotide (FAD)-binding motif. The hydration activities of S. maltophilia OhyA2 toward unsaturated fatty acids, including oleic acid, palmitoleic acid, linoleic acid, α-linolenic acid, and γ-linolenic acid, were greater than those of OhyA1. Moreover, the specific activity of S. maltophilia OhyA2 toward unsaturated fatty acids, with the exception of γ-linolenic acid, was the highest among all reported OhyAs.

Original languageEnglish
Article numbere03351-16
JournalApplied and Environmental Microbiology
Volume83
Issue number9
DOIs
StatePublished - 1 May 2017

Keywords

  • Enzyme characterization
  • Oleate hydratase
  • Stenotrophomonas maltophilia

Fingerprint

Dive into the research topics of 'Comparison of biochemical properties of the original and newly identified oleate hydratases from Stenotrophomonas maltophilia'. Together they form a unique fingerprint.

Cite this