Comparative study of the difference in behavior of the accessory gene regulator (agr) in USA300 and USA400 community-associated methicillin-resistant staphylococcus aureus (CA-MRSA)

Hye Soo Lee; Hun Suk Song; Hong Ju Lee; Sang Hyun Kim; Min Ju Suh; Jang Yeon Cho; Sion Ham; Yun Gon Kim; Hwang Soo Joo; Wooseong Kim; Sang Ho Lee; Dongwon Yoo; Shashi Kant Bhatia; Yung Hun Yang

doi:10.4014/jmb.2104.04032

Comparative study of the difference in behavior of the accessory gene regulator (agr) in USA300 and USA400 community-associated methicillin-resistant staphylococcus aureus (CA-MRSA)

Hye Soo Lee, Hun Suk Song, Hong Ju Lee, Sang Hyun Kim, Min Ju Suh, Jang Yeon Cho, Sion Ham, Yun Gon Kim, Hwang Soo Joo, Wooseong Kim, Sang Ho Lee, Dongwon Yoo, Shashi Kant Bhatia, Yung Hun Yang

Department of Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Community-associated Methicillin-Resistant Staphylococcus aureus (CA-MRSA) is notorious as a leading cause of soft tissue infections. Despite several studies on the Agr regulator, the mechanisms of action of Agr on the virulence factors in different strains are still unknown. To reveal the role of Agr in different CA-MRSA, we investigated the LACΔagr mutant and the MW2Δagr mutant by comparing LAC (USA300), MW2 (USA400), and Δagr mutants. The changes of Δagr mutants in sensitivity to oxacillin and several virulence factors such as biofilm formation, pigmentation, motility, and membrane properties were monitored. LACΔagr and MW2Δagr mutants showed different oxacillin sensitivity and biofilm formation compared to the LAC and MW2 strains. Regardless of the strain, the motility was reduced in Δagr mutants. And there was an increase in the long chain fatty acid in phospholipid fatty acid composition of Δagr mutants. Other properties such as biofilm formation, pigmentation, motility, and membrane properties were different in both Δagr mutants. The Agr regulator may have a common role like the control of motility and strain-dependent roles such as antibiotic resistance, biofilm formation, change of membrane, and pigment production. It does not seem easy to control all MRSA by targeting the Agr regulator only as it showed strain-dependent behaviors.

Original language	English
Pages (from-to)	1060-1068
Number of pages	9
Journal	Journal of Microbiology and Biotechnology
Volume	31
Issue number	8
DOIs	https://doi.org/10.4014/jmb.2104.04032
State	Published - 28 Aug 2021

Bibliographical note

Funding Information:
The authors would like to acknowledge the KU Research Professor Program of Konkuk University, Seoul, South Korea. This study was supported by the National Research Foundation of Korea (NRF) (NRF-2019R1F1A1058805, NRF-2019M3E6A1103979, 2021R1C1C2010609), and the Research Program to solve the social issues of the NRF funded by the Ministry of Science and ICT (2017M3A9E4077234). We thanks to Dr. Michael Otto. at Pathogen Molecular Genetics Section, Laboratory of Bacteriology, National Institute of Allergy and Infectious Diseases, U.S. National Institutes of Health, kindly giving us Staphylococcus aureus strains for our study.

Publisher Copyright:
Copyright © 2021 by The Korean Society for Microbiology and Biotechnology.

Keywords

CA-MRSA
Function of agr system
Strain-dependent behaviors
USA300
USA400

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10.4014/jmb.2104.04032

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Lee, H. S., Song, H. S., Lee, H. J., Kim, S. H., Suh, M. J., Cho, J. Y., Ham, S., Kim, Y. G., Joo, H. S., Kim, W., Lee, S. H., Yoo, D., Bhatia, S. K., & Yang, Y. H. (2021). Comparative study of the difference in behavior of the accessory gene regulator (agr) in USA300 and USA400 community-associated methicillin-resistant staphylococcus aureus (CA-MRSA). Journal of Microbiology and Biotechnology, 31(8), 1060-1068. https://doi.org/10.4014/jmb.2104.04032

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title = "Comparative study of the difference in behavior of the accessory gene regulator (agr) in USA300 and USA400 community-associated methicillin-resistant staphylococcus aureus (CA-MRSA)",

abstract = "Community-associated Methicillin-Resistant Staphylococcus aureus (CA-MRSA) is notorious as a leading cause of soft tissue infections. Despite several studies on the Agr regulator, the mechanisms of action of Agr on the virulence factors in different strains are still unknown. To reveal the role of Agr in different CA-MRSA, we investigated the LACΔagr mutant and the MW2Δagr mutant by comparing LAC (USA300), MW2 (USA400), and Δagr mutants. The changes of Δagr mutants in sensitivity to oxacillin and several virulence factors such as biofilm formation, pigmentation, motility, and membrane properties were monitored. LACΔagr and MW2Δagr mutants showed different oxacillin sensitivity and biofilm formation compared to the LAC and MW2 strains. Regardless of the strain, the motility was reduced in Δagr mutants. And there was an increase in the long chain fatty acid in phospholipid fatty acid composition of Δagr mutants. Other properties such as biofilm formation, pigmentation, motility, and membrane properties were different in both Δagr mutants. The Agr regulator may have a common role like the control of motility and strain-dependent roles such as antibiotic resistance, biofilm formation, change of membrane, and pigment production. It does not seem easy to control all MRSA by targeting the Agr regulator only as it showed strain-dependent behaviors.",

keywords = "CA-MRSA, Function of agr system, Strain-dependent behaviors, USA300, USA400",

author = "Lee, {Hye Soo} and Song, {Hun Suk} and Lee, {Hong Ju} and Kim, {Sang Hyun} and Suh, {Min Ju} and Cho, {Jang Yeon} and Sion Ham and Kim, {Yun Gon} and Joo, {Hwang Soo} and Wooseong Kim and Lee, {Sang Ho} and Dongwon Yoo and Bhatia, {Shashi Kant} and Yang, {Yung Hun}",

note = "Funding Information: The authors would like to acknowledge the KU Research Professor Program of Konkuk University, Seoul, South Korea. This study was supported by the National Research Foundation of Korea (NRF) (NRF-2019R1F1A1058805, NRF-2019M3E6A1103979, 2021R1C1C2010609), and the Research Program to solve the social issues of the NRF funded by the Ministry of Science and ICT (2017M3A9E4077234). We thanks to Dr. Michael Otto. at Pathogen Molecular Genetics Section, Laboratory of Bacteriology, National Institute of Allergy and Infectious Diseases, U.S. National Institutes of Health, kindly giving us Staphylococcus aureus strains for our study. Publisher Copyright: Copyright {\textcopyright} 2021 by The Korean Society for Microbiology and Biotechnology.",

year = "2021",

month = aug,

day = "28",

doi = "10.4014/jmb.2104.04032",

language = "English",

volume = "31",

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Lee, HS, Song, HS, Lee, HJ, Kim, SH, Suh, MJ, Cho, JY, Ham, S, Kim, YG, Joo, HS, Kim, W, Lee, SH, Yoo, D, Bhatia, SK & Yang, YH 2021, 'Comparative study of the difference in behavior of the accessory gene regulator (agr) in USA300 and USA400 community-associated methicillin-resistant staphylococcus aureus (CA-MRSA)', Journal of Microbiology and Biotechnology, vol. 31, no. 8, pp. 1060-1068. https://doi.org/10.4014/jmb.2104.04032

Comparative study of the difference in behavior of the accessory gene regulator (agr) in USA300 and USA400 community-associated methicillin-resistant staphylococcus aureus (CA-MRSA). / Lee, Hye Soo; Song, Hun Suk; Lee, Hong Ju et al.
In: Journal of Microbiology and Biotechnology, Vol. 31, No. 8, 28.08.2021, p. 1060-1068.

Research output: Contribution to journal › Article › peer-review

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AU - Lee, Hye Soo

AU - Song, Hun Suk

AU - Lee, Hong Ju

AU - Kim, Sang Hyun

AU - Suh, Min Ju

AU - Cho, Jang Yeon

AU - Ham, Sion

AU - Kim, Yun Gon

AU - Joo, Hwang Soo

AU - Kim, Wooseong

AU - Lee, Sang Ho

AU - Yoo, Dongwon

AU - Bhatia, Shashi Kant

AU - Yang, Yung Hun

N1 - Funding Information: The authors would like to acknowledge the KU Research Professor Program of Konkuk University, Seoul, South Korea. This study was supported by the National Research Foundation of Korea (NRF) (NRF-2019R1F1A1058805, NRF-2019M3E6A1103979, 2021R1C1C2010609), and the Research Program to solve the social issues of the NRF funded by the Ministry of Science and ICT (2017M3A9E4077234). We thanks to Dr. Michael Otto. at Pathogen Molecular Genetics Section, Laboratory of Bacteriology, National Institute of Allergy and Infectious Diseases, U.S. National Institutes of Health, kindly giving us Staphylococcus aureus strains for our study. Publisher Copyright: Copyright © 2021 by The Korean Society for Microbiology and Biotechnology.

PY - 2021/8/28

Y1 - 2021/8/28

N2 - Community-associated Methicillin-Resistant Staphylococcus aureus (CA-MRSA) is notorious as a leading cause of soft tissue infections. Despite several studies on the Agr regulator, the mechanisms of action of Agr on the virulence factors in different strains are still unknown. To reveal the role of Agr in different CA-MRSA, we investigated the LACΔagr mutant and the MW2Δagr mutant by comparing LAC (USA300), MW2 (USA400), and Δagr mutants. The changes of Δagr mutants in sensitivity to oxacillin and several virulence factors such as biofilm formation, pigmentation, motility, and membrane properties were monitored. LACΔagr and MW2Δagr mutants showed different oxacillin sensitivity and biofilm formation compared to the LAC and MW2 strains. Regardless of the strain, the motility was reduced in Δagr mutants. And there was an increase in the long chain fatty acid in phospholipid fatty acid composition of Δagr mutants. Other properties such as biofilm formation, pigmentation, motility, and membrane properties were different in both Δagr mutants. The Agr regulator may have a common role like the control of motility and strain-dependent roles such as antibiotic resistance, biofilm formation, change of membrane, and pigment production. It does not seem easy to control all MRSA by targeting the Agr regulator only as it showed strain-dependent behaviors.

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ER -

Comparative study of the difference in behavior of the accessory gene regulator (agr) in USA300 and USA400 community-associated methicillin-resistant staphylococcus aureus (CA-MRSA)

Abstract

Bibliographical note

Keywords

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