Comparative study of the difference in behavior of the accessory gene regulator (agr) in USA300 and USA400 community-associated methicillin-resistant staphylococcus aureus (CA-MRSA)

Hye Soo Lee, Hun Suk Song, Hong Ju Lee, Sang Hyun Kim, Min Ju Suh, Jang Yeon Cho, Sion Ham, Yun Gon Kim, Hwang Soo Joo, Wooseong Kim, Sang Ho Lee, Dongwon Yoo, Shashi Kant Bhatia, Yung Hun Yang

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Community-associated Methicillin-Resistant Staphylococcus aureus (CA-MRSA) is notorious as a leading cause of soft tissue infections. Despite several studies on the Agr regulator, the mechanisms of action of Agr on the virulence factors in different strains are still unknown. To reveal the role of Agr in different CA-MRSA, we investigated the LACΔagr mutant and the MW2Δagr mutant by comparing LAC (USA300), MW2 (USA400), and Δagr mutants. The changes of Δagr mutants in sensitivity to oxacillin and several virulence factors such as biofilm formation, pigmentation, motility, and membrane properties were monitored. LACΔagr and MW2Δagr mutants showed different oxacillin sensitivity and biofilm formation compared to the LAC and MW2 strains. Regardless of the strain, the motility was reduced in Δagr mutants. And there was an increase in the long chain fatty acid in phospholipid fatty acid composition of Δagr mutants. Other properties such as biofilm formation, pigmentation, motility, and membrane properties were different in both Δagr mutants. The Agr regulator may have a common role like the control of motility and strain-dependent roles such as antibiotic resistance, biofilm formation, change of membrane, and pigment production. It does not seem easy to control all MRSA by targeting the Agr regulator only as it showed strain-dependent behaviors.

Original languageEnglish
Pages (from-to)1060-1068
Number of pages9
JournalJournal of Microbiology and Biotechnology
Volume31
Issue number8
DOIs
StatePublished - 28 Aug 2021

Bibliographical note

Funding Information:
The authors would like to acknowledge the KU Research Professor Program of Konkuk University, Seoul, South Korea. This study was supported by the National Research Foundation of Korea (NRF) (NRF-2019R1F1A1058805, NRF-2019M3E6A1103979, 2021R1C1C2010609), and the Research Program to solve the social issues of the NRF funded by the Ministry of Science and ICT (2017M3A9E4077234). We thanks to Dr. Michael Otto. at Pathogen Molecular Genetics Section, Laboratory of Bacteriology, National Institute of Allergy and Infectious Diseases, U.S. National Institutes of Health, kindly giving us Staphylococcus aureus strains for our study.

Publisher Copyright:
Copyright © 2021 by The Korean Society for Microbiology and Biotechnology.

Keywords

  • CA-MRSA
  • Function of agr system
  • Strain-dependent behaviors
  • USA300
  • USA400

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