Comparative evaluation of two matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) systems, Vitek MS and Microflex LT, for the identification of Gram-positive cocci routinely isolated in clinical microbiology laboratories

Miae Lee; Hae Sun Chung; Hee Won Moon; Sun Hwa Lee; Kyungwon Lee

doi:10.1016/j.mimet.2015.03.020

Comparative evaluation of two matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) systems, Vitek MS and Microflex LT, for the identification of Gram-positive cocci routinely isolated in clinical microbiology laboratories

Miae Lee, Hae Sun Chung, Hee Won Moon, Sun Hwa Lee, Kyungwon Lee

Department of Laboratory Medicine

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

We evaluated the performance of two MALDI-TOF MS systems for the identification of clinically important Gram-positive cocci. Vitek MS and Microflex LT correctly identified 97.2% and 94.7%, respectively. Both systems offer reliable and rapid identification of clinically important Gram-positive cocci isolated in clinical laboratories, including staphylococci, streptococci, and enterococci. Expanding the databases, especially of coagulase-negative staphylococci and viridans streptococci, would enhance performance.

Original language	English
Pages (from-to)	13-15
Number of pages	3
Journal	Journal of Microbiological Methods
Volume	113
DOIs	https://doi.org/10.1016/j.mimet.2015.03.020
State	Published - 1 Jun 2015

Bibliographical note

Publisher Copyright:
© 2015 Elsevier B.V.

Keywords

Bacterial species identification
Gram-positive cocci
Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS)
Microflex LT
Vitek MS

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Lee, M., Chung, H. S., Moon, H. W., Lee, S. H., & Lee, K. (2015). Comparative evaluation of two matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) systems, Vitek MS and Microflex LT, for the identification of Gram-positive cocci routinely isolated in clinical microbiology laboratories. Journal of Microbiological Methods, 113, 13-15. https://doi.org/10.1016/j.mimet.2015.03.020

Lee, Miae ; Chung, Hae Sun ; Moon, Hee Won et al. / Comparative evaluation of two matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) systems, Vitek MS and Microflex LT, for the identification of Gram-positive cocci routinely isolated in clinical microbiology laboratories. In: Journal of Microbiological Methods. 2015 ; Vol. 113. pp. 13-15.

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title = "Comparative evaluation of two matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) systems, Vitek MS and Microflex LT, for the identification of Gram-positive cocci routinely isolated in clinical microbiology laboratories",

abstract = "We evaluated the performance of two MALDI-TOF MS systems for the identification of clinically important Gram-positive cocci. Vitek MS and Microflex LT correctly identified 97.2\% and 94.7\%, respectively. Both systems offer reliable and rapid identification of clinically important Gram-positive cocci isolated in clinical laboratories, including staphylococci, streptococci, and enterococci. Expanding the databases, especially of coagulase-negative staphylococci and viridans streptococci, would enhance performance.",

keywords = "Bacterial species identification, Gram-positive cocci, Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS), Microflex LT, Vitek MS",

author = "Miae Lee and Chung, \{Hae Sun\} and Moon, \{Hee Won\} and Lee, \{Sun Hwa\} and Kyungwon Lee",

note = "Publisher Copyright: {\textcopyright} 2015 Elsevier B.V.",

year = "2015",

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doi = "10.1016/j.mimet.2015.03.020",

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Lee, M, Chung, HS, Moon, HW, Lee, SH & Lee, K 2015, 'Comparative evaluation of two matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) systems, Vitek MS and Microflex LT, for the identification of Gram-positive cocci routinely isolated in clinical microbiology laboratories', Journal of Microbiological Methods, vol. 113, pp. 13-15. https://doi.org/10.1016/j.mimet.2015.03.020

Comparative evaluation of two matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) systems, Vitek MS and Microflex LT, for the identification of Gram-positive cocci routinely isolated in clinical microbiology laboratories. / Lee, Miae; Chung, Hae Sun; Moon, Hee Won et al.
In: Journal of Microbiological Methods, Vol. 113, 01.06.2015, p. 13-15.

Research output: Contribution to journal › Article › peer-review

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AU - Lee, Sun Hwa

AU - Lee, Kyungwon

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KW - Bacterial species identification

KW - Gram-positive cocci

KW - Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS)

KW - Microflex LT

KW - Vitek MS

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Lee M, Chung HS, Moon HW, Lee SH, Lee K. Comparative evaluation of two matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) systems, Vitek MS and Microflex LT, for the identification of Gram-positive cocci routinely isolated in clinical microbiology laboratories. Journal of Microbiological Methods. 2015 Jun 1;113:13-15. doi: 10.1016/j.mimet.2015.03.020