Comparative analysis of nonvolatile and volatile metabolites in Lichtheimia ramosa cultivated in different growth media

Hyun Chung, Nakyeom Lee, Jeong Ah Seo, Young Suk Kim

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15 Scopus citations


Lichtheimia ramosa is one of the predominant filamentous fungi in Korean traditional nuruk. The nonvolatile and volatile metabolites of L. ramosa cultivated in three growth media: complete medium (CM), potato dextrose broth (PDB), and sabouraud dextrose broth (SDB), were investigated and compared. Among nonvolatile metabolites, serine, lysine, and ornithine increased in CM and PDB cultivated with L. ramosa during the exponential phase. In addition, glucose level increased in CM whereas decreased in PDB and SDB. The major volatile metabolites in the extract samples were acetic acid, ethanol, 3-methyl-2-buten-1-ol, 2-phenylethanol, ethylacetate, 2-furaldehyde, 5-(hydroxymethyl)-2-furaldehyde, 2,3-dihydro-3,5,-dihydroxy-6-methyl-4Hpyran-4-one, and α-humulene. In particular, the levels of volatile metabolites related to makgeolli (e.g., acetic acid, ethanol, and ethyl acetate) were highest in extracts cultivated in CM. On the other hand, the level of 2-phenylethanol was relatively higher in PDB and SDB, possibly due to there being more phenylalanine present in the biomass sample in media.

Original languageEnglish
Pages (from-to)565-572
Number of pages8
JournalBioscience, Biotechnology and Biochemistry
Issue number3
StatePublished - 2017

Bibliographical note

Funding Information:
This work was supported by the Ministry of Agriculture, Food and Rural Affairs (Strategic Initiative for Microbiomes in Agriculture and Food) [grant number 914007-4]; the Technological innovation RandD program of SMBA [grant number S2340863].

Publisher Copyright:
© 2016 Japan Society for Bioscience, Biotechnology, and Agrochemistry.


  • Lichtheimia ramosa
  • Metabolites
  • Nonvolatile
  • Volatile


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