TY - JOUR
T1 - Comparative analysis of cigarette smoke induced cellular proteome distributions on bovine aortic endothelial cells
AU - Min, Jiho
AU - Ahn, Ji Young
AU - Sekhon, Simranjeet Singh
AU - Jin, Yoon Mi
AU - Um, Hyun Ju
AU - Jo, Inho
AU - Kim, Yang Hoon
PY - 2014/6
Y1 - 2014/6
N2 - Cigarette smoke is a contributory factor for the cardiovascular disease, lung diseases and cancers as the dominant illnesses. The proteomic analysis of bovine aortic endothelial cells (BAECs) exposed to cigarette smoke has been performed in the broad (Non-linear pH 3-11) and narrow (Linear pH 4-7) range by using two-dimensional gel electrophoresis (2-DE). Out of an average 950 spots observed in 2-DE gels under pH 3-11, the expression level of 25 proteins significantly increased with an increase of cigarette smoke extract (CSE) level, whereas 21 proteins were strongly down-regulated. In narrow (4-7) pH range analysis, 80 proteins showed a significant change in expression level as compared with control. Some of the proteins were found to show similar spot intensity patterns in both the linear (4-7) and nonlinear (3-11) pH ranges. Most of the proteins identified in both pH conditions were found to be involved in apoptosis, inflammation, transcription modulator, signal transduction pathway, ROS production, cell proliferation and extracellular structure formation. In addition, extracellular structural proteins also responded to apoptotic signaling as an indicator. The findings of the present study can be used as an early biomarker to indicate the risks of cigarette smoke related diseases and also in the design of new therapeutic and diagnostic approaches to control these diseases.
AB - Cigarette smoke is a contributory factor for the cardiovascular disease, lung diseases and cancers as the dominant illnesses. The proteomic analysis of bovine aortic endothelial cells (BAECs) exposed to cigarette smoke has been performed in the broad (Non-linear pH 3-11) and narrow (Linear pH 4-7) range by using two-dimensional gel electrophoresis (2-DE). Out of an average 950 spots observed in 2-DE gels under pH 3-11, the expression level of 25 proteins significantly increased with an increase of cigarette smoke extract (CSE) level, whereas 21 proteins were strongly down-regulated. In narrow (4-7) pH range analysis, 80 proteins showed a significant change in expression level as compared with control. Some of the proteins were found to show similar spot intensity patterns in both the linear (4-7) and nonlinear (3-11) pH ranges. Most of the proteins identified in both pH conditions were found to be involved in apoptosis, inflammation, transcription modulator, signal transduction pathway, ROS production, cell proliferation and extracellular structure formation. In addition, extracellular structural proteins also responded to apoptotic signaling as an indicator. The findings of the present study can be used as an early biomarker to indicate the risks of cigarette smoke related diseases and also in the design of new therapeutic and diagnostic approaches to control these diseases.
KW - 2-dimensional electrophoresis (2-DE)
KW - Cigarette smoke
KW - Comparative proteomics
KW - Endothelial cells
UR - http://www.scopus.com/inward/record.url?scp=84940241073&partnerID=8YFLogxK
U2 - 10.1007/s13273-014-0015-x
DO - 10.1007/s13273-014-0015-x
M3 - Article
AN - SCOPUS:84940241073
SN - 1738-642X
VL - 10
SP - 135
EP - 148
JO - Molecular and Cellular Toxicology
JF - Molecular and Cellular Toxicology
IS - 2
ER -