Combined effects of 60Hz electromagnetic field exposure with various stress factors on cellular transformation in NIH3T3 cells

Hae June Lee, Yeung Bae Jin, Jae Seon Lee, Jong Il Choi, Ju Woon Lee, Sung Ho Myung, Yun Sil Lee

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Epidemiological studies have suggested that extremely low-frequency magnetic fields (ELF-MF) are associated with an increased incidence of cancer. Studies using in vitro systems have reported mixed results for the effects of ELF-MF alone, and the World Health Organization (WHO) Research Agenda published in 2007 suggested that high priority research should include an evaluation of the co-carcinogenic effects of ELF-MF exposure using in vitro models. Here, the carcinogenic potential of ELF-MF exposure alone and in combination with various stress factors was investigated in NIH3T3 mouse fibroblasts using an in vitro cellular transformation assay. NIH3T3 cells were exposed to a 60Hz ELF-MF (1mT) alone or in combination with ionizing radiation (IR), hydrogen peroxide (H 2O 2), or c-Myc overexpression, and the resulting number of anchorage-independent colonies was counted. A 4h exposure of NIH3T3 cells to ELF-MF alone produced no cell transformation. Moreover, ELF exposure did not influence the transformation activity of IR, H 2O 2, or activated c-Myc in our in vitro assay system, suggesting that 1mT ELF-MF did not affect any additive or synergistic transformation activities in combination with stress factors such as IR, H 2O 2, or activated c-Myc in NIH3T3 cells.

Original languageEnglish
Pages (from-to)207-214
Number of pages8
JournalBioelectromagnetics
Volume33
Issue number3
DOIs
StatePublished - Apr 2012

Keywords

  • Cellular transformation
  • Combination with stress factors
  • Extremely low frequency

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